Microbial bio-inoculation effects on the seed germination dynamics and field performance of pea (Pisum sativum L.) under osmotic stress and fertilization in the Amazonas region of Peru

Abstract

Microbial bio-inoculants have been proposed as management tools to enhance crop performance under variable environmental conditions; however, their effectiveness is often influenced by site-specific factors. This study evaluated the effects of bio-inoculation on seed germination and seedling vigor of pea under osmotic stress induced by polyethylene glycol (PEG 6000), and its interaction with two fertilization levels (75% and 100% of the recommended dose) under field conditions in the Amazonas region of Peru. Under laboratory conditions, germination percentage remained high across all treatments (93.3–100%) and was not affected by bio-inoculation or osmotic potential; however, osmotic stress altered germination dynamics, increasing mean germination time from 1.85–2.09 days at 0 MPa to 2.26–2.43 days at −0.8 MPa, while germination synchrony and seedling vigor decreased as stress increased. The seedling vigor index reached maximum values at −0.2 MPa (4.47–5.29) and declined at −0.8 MPa (1.50–2.00), and multivariate analyses showed that variation in germination responses was mainly associated with germination timing and vigor rather than seed viability. Under field conditions, no significant effects of fertilization level, microbial bio-inoculation, or their interaction were detected on agronomic traits or yield, although variability between locations was observed; plant height ranged from 38.5–46.3 cm in Lamud and from 100.6–108.3 cm in Molinopampa, while grain yield varied from 698–1846 kg/ha and 8771–9919 kg/ha, respectively. Overall, environmental conditions exerted a stronger influence than microbial bio-inoculation on germination dynamics and field productivity, while the findings provide practical guidance for improving pea production with bio-inoculants and optimized fertilization.