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dc.contributor.authorCastro Gómez, Juan Carlos-
dc.contributor.authorCobos Ruiz, Marianela-
dc.contributor.authorMaddox, J. Dylan-
dc.contributor.authorImán Correa, Sixto Alfredo-
dc.contributor.authorMarapara del Aguila, Jorge Luis-
dc.identifier.citationCastro, J.; Cobos, M.; Maddox, J.; Imán, S. A. & Marapara, J. L. (2016). Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C. Plant Cell Biotechnology and Molecular Biology, 17(7-8), 415-427. Disponible en http://www.ikpress.org/index.php/PCBMB/article/view/1647es_PE
dc.description.abstractMyrciaria dubia “camu-camu” is a rich source of several bioactive phytochemicals and vitamin C (L-ascorbic acid, AsA). To gain insights about the genes involved in AsA biosynthesis in this plant species and consequently with potential use for its biotechnological production, here we report the isolation and molecular cloning of partial gene sequences of the D-mannose/L-galactose pathway. Degenerate primers designed by the multiple sequence alignment of related plant species were used to isolate in M. dubia the partial sequences of the six D-mannose/L-galactose pathway genes (GMP, GME, GGP, GPP, GDH and GLDH). The deduced protein sequences of the six genes have more than 81% sequence identity to rosids and asterids species, with a closer phylogenetic relationship to Eucalyptus grandis. In conclusion, gene sequences of the D-mannose/L-galactose pathway involved in AsA biosynthesis of M. dubia were successfully isolated and cloned and the phylogenetic analysis indicated that these genes have been relatively well conserved throughout of plant evolution, reflecting the importance of the enzymes of this metabolic pathway for plant growth and survival. Additionally, the isolation and cloning of these genes allow us to implement systems for biotechnological production of AsA.es_PE
dc.publisherSociety for Biology and Biotechnologyes_PE
dc.relation.ispartofPlant Cell Biotechnology and Molecular Biology, vol.17, n.7-8, pp.415-427; 2016es_PE
dc.sourceInstituto Nacional de Innovación Agrariaes_PE
dc.source.uriRepositorio Institucional - INIAes_PE
dc.subjectClonación moleculares_PE
dc.subjectBiotecnología moleculares_PE
dc.subjectFilogenia moleculares_PE
dc.subjectBiosíntesis del ácido L-ascórbicoes_PE
dc.subjectFrutas tropicaleses_PE
dc.subjectGene cloninges_PE
dc.subjectMolecular biotechnologyes_PE
dc.subjectMolecular phylogenyes_PE
dc.subjectL-ascorbic acid biosynthesises_PE
dc.subjectTropical fruites_PE
dc.titleIsolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin Ces_PE
dc.subject.ocdeRecursos genéticoses_PE
dc.identifier.journalPlant Cell Biotechnology and Molecular Biologyes_PE
dc.description.peer-reviewPeer reviewedes_PE
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