Artículos producto de proyectos financiados

URI permanente para esta colecciónhttps://repositorio.inia.gob.pe/handle/20.500.12955/2241

Esta colección contiene artículos científicos resultantes o derivados de proyectos con financiamiento PNIA/INIA y cuyos autores no tiene filiación institucional.

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Physiological characterization and bioactive compounds of promising accessions of cowpea (Vigna unguiculata L. Walp) in the Peruvian Amazon
(Frontiers Media S.A., 2024-05-07) Murga Orrillo, Hipolito; Chuquímez Gonzales, Jhon Kevin; Arévalo López, Luis Alberto
Cowpea production in the Peruvian Amazon is increasing due to its ability to adapt to diverse environments, its contribution to soil conservation, and its versatility of uses. This crop is particularly valuable in human nutrition due to its high nutritional quality and nutraceutical properties of its bioactive compounds. The aim of the study was to evaluate the physiology of cowpea plants in accessions PER1005854, PER1005851, and PER12645, under the influence of rice husk mulch, screw tree litter, and oil palm fiber, as well as to determine the bioactive compounds present in the grains. This study aims to promote sustainable cowpea production, expose the nutritional aspects of the grains, and foster their consumption both locally and nationally. The experiment was conducted from November 2022 to March 2023, in Yurimaguas, Loreto, Peru. The application of organic mulches demonstrates a significantly positive impact on the growth of cowpea accessions during their vegetative phase. This effect is possibly attributed to increased water availability, as the mulch reduces soil evaporation. In particular, cowpea accessions PER12645 and PER1005851 exhibit desirable physiological characteristics for grain production, with short cycles of 71 days and higher yields of 1141 and 1125 kg/ha respectively. In contrast, accession PER1005854 is distinguished by its higher biomass production, reaching a value of 14497 kg/ha, a relevant trait for foliage production and its contribution to soil conservation. The bioactive compounds, such as proteins, lipids, and carbohydrates, present in the accessions PER1005854, PER1005851, and PER12645, are similar to those found in other legumes. However, the antioxidant activity of dark tegument cowpeas proved to be superior, particularly highlighted in accession PER12645 (black tegument) with 26.3 μmolTG/g, and in accession PER1005854 (dark red tegument) with 19.5 μmolTG/g. This characteristic is particularly important for consumption, as it is related to the ability to combat oxidative stress in the human body.
The effect of hydrothermal treatment on metabolite composition of hass avocados stored in a controlled atmosphere
(MDPI, 2021-11-10) Chirinos, Rosana; Campos, David; Martínez, Sofía; Llanos, Sílfida; Betalleluz Pallardel, Indira; García Ríos, Diego; Pedreschi, Romina
Avocado cv. Hass consumption has expanded worldwide given its nutritional, sensory, and functional attributes. In this work, avocado fruit from two harvests was subjected to hydrothermal treatment (38 °C for 1 h) or left untreated (control) and then stored for 30 and 50 days in a controlled atmosphere (4 kPa O2 and 6 kPa CO2 at 7 °C) (HTCA and CA, respectively) with subsequent ripening at ~20 °C. The fruit was evaluated for primary and secondary metabolites at harvest, after storage, and after reaching edible ripeness. A decrease from harvest to edible ripeness in mannoheptulose and perseitol was observed while β-sitosterol, hydrophilic and lipophilic antioxidant activity (H-AOX, L-AOX), abscisic acid, and total phenolics (composed of p-coumaric and caffeic acids such as aglycones or their derivatives) increased. HTCA fruit at edible ripeness displayed higher contents of mannoheptulose, perseitol, β-sitosterol, L-AOX, caffeic acid, and p-coumaric acid derivatives, while CA fruit presented higher contents of α-tocopherol, H-AOX, and syringic acid glycoside for both harvests and storage times. The results indicate that a hydrothermal treatment prior to CA enables fruit of high nutritional value characterized by enhanced content of phenolic compounds at edible ripeness to reach distant markets.
Tendencia genética y fenotípica de la producción de leche: caso de un establo comercial del valle de Huaura, Perú
(Corporación Colombiana de Investigación Agropecuaria, 2021-03-27) Hidalgo Vásquez, Yaneline Nilda; García Salas, María Elisa Catalina; Gutiérrez Reynoso, Gustavo Augusto; Chagray Ameri, Néstor Humberto
El objetivo de esta investigación fue determinar la tendencia genética y fenotípica de la producción de leche (PL) de vacas Holstein de un establo del valle de Huaura, Perú. Se evaluaron 2.590 hembras (vacas y terneras) para el cálculo de los valores genéticos estimados (VGE) y 2.862 lactaciones estandarizadas a 305 días y dos ordeños diarios, del primer al quinto parto de 1.892 vacas del periodo 1999-2017, mediante un modelo animal de medidas repetidas. Con el software ASReml, se estimó una heredabilidad (h2) de 0,16 (error estándar 0,03) y una repetibilidad (r) de 0,28 (0,025). Las tendencias se estimaron mediante regresión lineal usando el paquete estadístico SAS v.9.4. Los VGE para la PL de las vacas y terneras tienen una media de +200,9 (16,1) kg y +148,7 (9,7) kg, respectivamente, con más del 70 % de valores positivos. La tendencia genética para PL de las hembras fue -2,4 (2,1) kg/año. Se estimó una tendencia fenotípica para PL de +294,3 (24,9) kg/año. Se concluye que la tendencia fenotípica es favorable debido a mejoras en aspectos no genéticos, ya que la h2 indica que la PL está influenciada principalmente por el ambiente y, en menor medida, por la varianza genética aditiva del carácter. La tendencia genética para la PL de hembras fue negativa. Sin embargo, los VGE para PL de las hembras vivas sugieren que a futuro, el establo puede incrementar la tendencia genética para PL.
Variations on a theme: two structural motifs create species-specific pheromone channels for multiple species of South American cerambycid beetles
(MDPI, 2020-04-02) Silva, Weliton D.; Hanks, Lawrence M.; Alvarez, Jean Carlos S.; Madalon, Fernando Z.; Bento, José Maurício S.; Bello, Jan E.; Millar, Jocelyn G.
We describe the identification, synthesis, and field-testing of aggregation-sex pheromones, or likely pheromone candidates, of seven species of South American cerambycid beetles in the subfamily Cerambycinae, of the tribes Eburiini and Neoibidionini. Analyses of extracts of volatiles released by adult males revealed that Eburodacrys dubitata White produce 11-methyltridecanal, whereas the males of Eburodacrys assimilis Gounelle, Eburodacrys flexuosa Gounelle, and Eburodacrys lenkoi Napp and Martins produce blends of this compound, along with its analog 10-methyldodecanal. In contrast, males of Compsibidion graphicum (Thomson) and Compsibidion sommeri (Thomson) produce blends of 10-methyldodecanal and its corresponding alcohol 10-methyldodecanol. The results from field bioassays with synthetic compounds showed that each species was specifically attracted to traps containing their reconstructed pheromone blend. However, E. assimilis was not trapped, possibly due to inhibition by non-natural enantiomers in the racemic test compounds. During the trials for the Compsibidion species, adults of another cerambycid species, Tetraopidion mucoriferum (Thomson), were captured in significant numbers in traps baited with 10-methyldodecanol, suggesting that this compound is a pheromone component for this species. This study demonstrates another case of conservation of pheromone structures within South American cerambycid species. It also highlights how blends of closely related structures, differing only in chain length or functional group, make the evolution of species-specific pheromone channels possible.
Mass spectrometry-based flavor monitoring of Peruvian chocolate fabrication process
(MDPI, 2021-01-26) Michel, Stephanie; Baraka, Luka Franco; Ibañez, Alfredo J.; Mansurova, Madina
Flavor is one of the most prominent characteristics of chocolate and is crucial in determining the price the consumer is willing to pay. At present, two types of cocoa beans have been characterized according to their flavor and aroma profile, i.e., (1) the bulk (or ordinary) and (2) the fine flavor cocoa (FFC). The FFC has been distinguished from bulk cocoa for having a great variety of flavors. Aiming to differentiate the FFC bean origin of Peruvian chocolate, an analytical methodology using gas chromatography coupled to mass spectrometry (GC-MS) was developed. This methodology allows us to characterize eleven volatile organic compounds correlated to the aromatic profile of FFC chocolate from this geographical region (based on buttery, fruity, floral, ethereal sweet, and roasted flavors). Monitoring these 11 flavor compounds during the chain of industrial processes in a retrospective way, starting from the final chocolate bar towards pre-roasted cocoa beans, allows us to better understand the cocoa flavor development involved during each stage. Hence, this methodology was useful to distinguish chocolates from different regions, north and south of Peru, and production lines. This research can benefit the chocolate industry as a quality control protocol, from the raw material to the final product.
Bioprocessing of organic wastes from poultry and bovine slaughterhouses as food substrate for Hermetia illucens larval development
(Global Journal of Environmental Science and Management, 2023-01-31) Luperdi, A. P.; Flores Calla, S. S.; Barriga, X. J.; Rivera, V.; Salazar, I.; Manrique, P. L.; Reátegui, J. E.
BACKGROUND AND OBJECTIVES: In the meat industry, inefficient management of organic waste exists, therefore the study aims to evaluate different bovine and poultry organic residues as food substrates during larval development of the black soldier fly, such as a sustainable alternative to obtain high protein meal. METHODS: The research evaluates the use of organic waste from cattle and poultry slaughterhouses, as food substrate for black soldier fly larvae, including raw beef blood T1, raw beef viscera T2, cooked beef blood T3, cooked beef viscera T4, raw chicken viscera T6 and cooked chicken viscera T7; further, as a control measure balanced feed (7 treatments and 5 replicates). Larvae were fed for 5 days and processed to make meal by drying and grinding; evaluating mortality, weight, size, proximal chemical composition, and apparent digestibility to determine the most viable substrate, analyzing effects and significance by multifactorial ANOVA and Kruskal-Wallis. FINDING: The results show Mortality (F = 917,81, p < 0,0001): T1 y T3 with 76,40 ± 2,86 (%) (F = 917,81, p < 0,0001), following T6 with 69,67 ± 4,55%, T7 with 24,00 ± 3,48%, T2 with 4,60 ± 1,92 %, T5 y T4, both with 4,20 ± 2,00 %. Weight (F = 825,62, p < 0,0001): T2 with 1,78 ± 0,22 gram outperformed the control T5 (1,76 ± 0,50 gram), T4 with 1,45 ± 0,06 g and T7 with 1,66 ± 0,07 gram. Size (F = 248,95, p < 0,0001): T5 with 16,03 ± 0,34 mm, T2 with 15,86 ± 0,22 mm, T4 with 14,72 ± 0,35 mm and finally, 14,51 ± 0,14 millimeter in T7. Proximal chemical analysis of crude protein and fat: T2 resulted in the following results 50,81 % and 21,88 %, T4 with 53,90% y 15,04%, T7 with 42,63 % and 32,03%, and T5 con 41,1 % and 19.55%, respectively. Digestibility: T5 with 20,39%, T2 with 12,66%, T4 with 10,61% and T7 with 5,97%. T2 raw beef viscera were determined to be the most viable substrate, followed by T4 cooked beef viscera and T7 cooked chicken viscera. CONCLUSIONS: Testing the effectiveness of cattle viscera as substrate, the experimental data presented may help design a process for an effective treatment method for slaughterhouse waste, which might benefit developing nations in managing their waste effectively, generating high protein meal, with the potential for a circular bioeconomy.
The phylogeography of potato virus X shows the fingerprints of its human vector
(MDPI, 2021-09-09) Fuentes, Segundo; Gibbs, Adrian J.; Hajizadeh, Mohammad; Perez, Ana; Adams, Ian Patrick; Fribourg, Cesar E.; Kreuze, Jan; Fox, Adrian; Boonham, Neil; Jones, Roger A. C.
Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop′s main domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes plus 49 published genomic sequences were analyzed. Only 18 of them were recombinants, 17 of them Peruvian. A phylogeny of the non-recombinant sequences found two major (I, II) and five minor (I-1, I-2, II-1, II-2, II-3) phylogroups, which included 12 statistically supported clusters. Analysis of 488 coat protein (CP) gene sequences, including 128 published previously, gave a completely congruent phylogeny. Among the minor phylogroups, I-2 and II-3 only contained Andean isolates, I-1 and II-2 were of both Andean and other isolates, but all of the three II-1 isolates were European. I-1, I-2, II-1 and II-2 all contained biologically typed isolates. Population genetic and dating analyses indicated that PVX emerged after potato’s domestication 9000 years ago and was transported to Europe after the 15th century. Major clusters A–D probably resulted from expansions that occurred soon after the potato late-blight pandemic of the mid-19th century. Genetic comparisons of the PVX populations of different Peruvian Departments found similarities between those linked by local transport of seed potato tubers for summer rain-watered highland crops, and those linked to winter-irrigated crops in nearby coastal Departments. Comparisons also showed that, although the Andean PVX population was diverse and evolving neutrally, its spread to Europe and then elsewhere involved population expansion. PVX forms a basal Potexvirus genus lineage but its immediate progenitor is unknown. Establishing whether PVX′s entirely Andean phylogroups I-2 and II-3 and its Andean recombinants threaten potato production elsewhere requires future biological studies.
Development of an open-source thermal image processing software for improving irrigation management in potato crops (Solanum tuberosum L.)
(MDPI, 2020-01-14) Cucho Padin, Gonzalo; Rinza, Javier; Ninanya, Johan; Loayza, Hildo; Quiroz, Roberto; Ramirez, David A.
Accurate determination of plant water status is mandatory to optimize irrigation scheduling and thus maximize yield. Infrared thermography (IRT) can be used as a proxy for detecting stomatal closure as a measure of plant water stress. In this study, an open-source software (Thermal Image Processor (TIPCIP)) that includes image processing techniques such as thermal-visible image segmentation and morphological operations was developed to estimate the crop water stress index (CWSI) in potato crops. Results were compared to the CWSI derived from thermocouples where a high correlation was found (𝑟𝑃𝑒𝑎𝑟𝑠𝑜𝑛 = 0.84). To evaluate the effectiveness of the software, two experiments were implemented. TIPCIP-based canopy temperature was used to estimate CWSI throughout the growing season, in a humid environment. Two treatments with different irrigation timings were established based on CWSI thresholds: 0.4 (T2) and 0.7 (T3), and compared against a control (T1, irrigated when soil moisture achieved 70% of field capacity). As a result, T2 showed no significant reduction in fresh tuber yield (34.5 ± 3.72 and 44.3 ± 2.66 t ha−1), allowing a total water saving of 341.6 ± 63.65 and 515.7 ± 37.73 m3 ha−1 in the first and second experiment, respectively. The findings have encouraged the initiation of experiments to automate the use of the CWSI for precision irrigation using either UAVs in large settings or by adapting TIPCIP to process data from smartphone-based IRT sensors for applications in smallholder settings.
The kinetics of total phenolic content and monomeric Flavan-3-ols during the roasting process of Criollo Cocoa
(MDPI, 2020-02-02) Fernández Romero, Editha; Chavez Quintana, Segundo G.; Siche, Raúl; Castro Alayo, Efraín M.; Cardenas Toro, Fiorella P.
Cocoa beans are the main raw material for the manufacture of chocolate and are currently gaining great importance due to their antioxidant potential attributed to the total phenolic content (TPC) and the monomeric flavan-3-ols (epicatechin and catechin). The objective of this study was to determine the degradation kinetics parameters of TPC, epicatechin, and catechin during the roasting process of Criollo cocoa for 10, 20, 30, 40, and 50 min at 90, 110, 130, 150, 170, 190, and 200 °C. The results showed a lower degradation of TPC (10.98 ± 6.04%) and epicatechin (8.05 ± 3.01%) at 130 °C and 10 min of roasting, while a total degradation of epicatechin and a 92.29 ± 0.06% degradation of TPC was obtained at 200 °C and 50 min. Reaction rate constant (𝑘) and activation energy (𝐸𝑎) were 0.02–0.10 min−1 and 24.03 J/mol for TPC and 0.02–0.13 min−1 and 22.51 J/mol for epicatechin, respectively. Degradation kinetics of TPC and epicatechin showed first-order reactions, while the catechin showed patterns of formation and degradation.
Canopy temperature as a key physiological trait to improve yield prediction under water restrictions in potato
(MDPI, 2021-07-20) Ninanya, Johan; Ramírez, David A.; Rinza, Javier; Silva-Díaz, Cecilia; Cervantes, Marcelo; García, Jerónimo; Quiroz, Roberto
Canopy temperature (CT) as a surrogate of stomatal conductance has been highlighted as an essential physiological indicator for optimizing irrigation timing in potatoes. However, assessing how this trait could help improve yield prediction will help develop future decision support tools. In this study, the incorporation of CT minus air temperature (dT) in a simple ecophysiological model was analyzed in three trials between 2017 and 2018, testing three water treatments under drip (DI) and furrow (FI) irrigations. Water treatments consisted of control (irrigated until field capacity) and two-timing irrigation based on physiological thresholds (CT and stomatal conductance). Two model perspectives were implemented based on soil water balance (P1) and using dT as the penalizing factor (P2), affecting the biomass dynamics and radiation use efficiency parameters. One of the trials was used for model calibration and the other two for validation. Statistical indicators of the model performance determined a better yield prediction at harvest for P2, especially under maximum stress conditions. The P1 and P2 perspectives showed their highest coefficient of determination (R2) and lowest root-mean-squared error (RMSE) under DI and FI, respectively. In the future, the incorporation of CT combining low-cost infrared devices/sensors with spatial crop models, satellite image information, and telemetry technologies, an adequate decision support system could be implemented for water requirement determination and yield prediction in potatoes.
Water saving using thermal imagery-based thresholds for timing irrigation in potatoes under drip and furrow irrigation systems
(MDPI, 2022-11-23) Rinza, Javier; Ramírez, David A.; Ninanya, Johan; De Mendiburu, Felipe; García, Jerónimo; Quiroz, Roberto
Under the current water crisis in agriculture, irrigation methods for saving and conserving water are necessary. However, these methods must guarantee an appropriate yield with a concomitant economic benefit and a reduced environmental impact. In this study, two irrigation thresholds for irrigation timing (IT) based on thermal imagery were analyzed with the UNICA potato variety in three trials under drip (DI) and furrow (FI) irrigation during 2017–2018 in Lima, Peru. The control (T1) remained at >70% of soil field capacity. For other treatments, thresholds were defined based on stomatal conductance at light saturation (T2: 0.15 and T3: 0.05 mol H2O m−2 s−1) and crop water stress index (T2: 0.4 and T3: 0.6) based on canopy temperature. An integrated index (IIN) was established for the valuation of treatments using the criteria of high fresh tuber yield (FTY) and a low total amount of irrigated water, production cost (PC), and total C emissions (TE) and using criteria of a score. FI-T2 (0.69–0.72) and DI-T3 (0.19–0.29) showed the highest and lowest IIN value, respectively. FTY in T2 was not significantly reduced under FI, resulting in a lower PC regarding DI–T2 and emphasizing the usefulness of thermal imagery in determining watering schedules in potatoes under furrow irrigation systems.
In-depth genetic diversity and population structure of endangered Peruvian Amazon rosewood germplasm using Genotyping by Sequencing (GBS) technology
(MDPI, 2021-02-08) Azhar Nadeem, Muhammad; Vasquez Guizado, Stalin Juan; Qasim Shahid, Muhammad; Amjad Nawaz, Muhammad; Habyarimana, Ephrem; Ercişli, Sezai; Ali, Fawad; Karaköy, Tolga; Aasim, Muhammad; Hatipoğlu, Rüştü; Castro Gómez, Juan Carlos; Marapara Del Aguila, Jorge Luis; Adrianzén Julca, Pedro Marcelino; Torres Canales, Esperanza; Hwan Yang, Seung; Chung, Gyuhwa; Shehzad Baloch, Faheem
Research studies on conservative genetics of endangered plants are very important to establish the management plans for the conservation of biodiversity. Rosewood is an evergreen tree of the Amazon region and its essential oil has great acceptance in the medical and cosmetic industry. The present study aimed to explore the genetic diversity and population structure of 90 rosewood accessions collected from eight localities of Peruvian Amazon territory through DArTseq markers. A total of 7485 informative markers resulted from genotyping by sequencing (GBS) analysis were used for the molecular characterization of rosewood germplasm. Mean values of various calculated diversity parameters like observed number of alleles (1.962), the effective number of alleles (1.669), unbiased expected heterozygosity (0.411), and percent polymorphism (93.51%) over the entire germplasm showed the existence of a good level of genetic variations. Our results showed that the Mairiricay population was more diverse compared to the rest of the populations. Tamshiyacu-2 and Mairiricay-15 accessions were found genetically distinct accessions. The analysis of molecular variance (AMOVA) reflected maximum variations (75%) are due to differences within populations. The implemented clustering algorithms, i.e.; STRUCTURE, neighbor-joining analysis and principal coordinate analysis (PCoA) separated the studied germplasm on the basis of their geographical locations. Diversity indices for STRUCTURE-based populations showed that subpopulation A is more diverse population than the rest of the populations, for such reason, individuals belonging to this subpopulation should be used for reintroduction or reinforcement plans of rosewood conservation. We envisage that molecular characterization of Peruvian rosewood germplasm with DArTseq markers will provide a platform for the conservation, management and restoration of endangered rosewood in upcoming years.
Preparation and characterization of licorice-chitosan coatings for postharvest treatment of fresh strawberries
(MDPI, 2020-11-26) Quintana, Somaris E.; Llalla, Olimpia; García Zapateiro, Luis A.; García Risco, Mónica R.; Fornari, Tiziana
Several plant extracts are being investigated to produce edible coatings, mainly due to their antioxidant and antimicrobial activities. In this study, licorice root extracts were produced by ultrasound-assisted extraction and were combined with chitosan to elaborate edible coatings. Different solvents and temperatures were used in the extraction process, and the antioxidant and antimicrobial activity of the extracts were assessed. The most bioactive extracts were selected for the development of the edible coatings. The rheological properties of the coatings were studied, and they were applied on strawberry to evaluate their physicochemical and microbiological properties. The addition of licorice extract to chitosan resulted in positive effects on the rheological properties of the coatings: the incorporation of phytochemicals to chitosan decreased the shear stress and improved the restructuring ability of the coating solutions. The films presented a reduction of the Burger model parameter, indicating a reduction of rigidity. Furthermore, the strawberry coated with chitosan and licorice extract maintained good quality parameters during storage and showed the best microbiological preservation in comparison with controls. Hence, the use of chitosan with licorice extract is a potential strategy to produce edible coating for improving the postharvest quality of fruits.
Unraveling ecophysiological mechanisms in potatoes under different irrigation methods: a preliminary field evaluation
(MDPI, 2020-06-11) Silva Díaz, Cecilia; Ramírez, David A.; Rodríguez Delfín, Alfredo; De Mendiburu, Felipe; Rinza, Javier; Ninanya, Johan; Loayza, Hildo; Quiroz, Roberto
Potatoes—a global food security and staple crop—is threatened by dry spells in drought-prone areas. The use of physiological thresholds to save water while maintaining a reasonable tuber yield has been proposed, but their effects on physiological performances and usefulness under different irrigation methods are yet to be evaluated. In this study, photosynthetic traits were monitored to assess the effect of water restriction and rewatering under drip (DI) and furrow (FI) irrigations. The treatments consisted of two maximum light-saturated stomatal conductance (g𝑠_𝑚𝑎𝑥) irrigation thresholds (T2: 0.15 and T3: 0.05 mol H2O m−2 s−1) compared with a fully irrigated control (g𝑠_𝑚𝑎𝑥 > 0.3 mol H2O m−2 s−1). DI used less water than FI but promoted early senescence and low percentage of maximum assimilation rate (PMA) at late developmental stages. FI caused no yield penalization in T2 and higher recovery of carbon isotope discrimination and PMA than DI. It is suggested that moderate water quantities of early and frequently water pulses in the irrigation, promote short-term water stress memory improvement, senescence delay and more capability of recovery at late stages.

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