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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Santiani, Alexei | - |
dc.contributor.author | Huanca López, Wilfredo | - |
dc.contributor.author | Sapana Valdivia, Rómulo | - |
dc.contributor.author | Huanca Mamani, Teodosio | - |
dc.contributor.author | Sepúlveda, Néstor | - |
dc.contributor.author | Sánchez, Raúl | - |
dc.date.accessioned | 2023-06-05T17:10:22Z | - |
dc.date.available | 2023-06-05T17:10:22Z | - |
dc.date.issued | 2005-09-01 | - |
dc.identifier.citation | Santiani, A.; Huanca, W.; Sapana, R.; Huanca, T.; Sepúlveda, N.; & Sánchez, R. (2005). Effects on the quality of frozen‐thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders. Asian journal of Andrology, 7(3), 303-309. doi: 10.1111/j.1745-7262.2005.00021.x | en |
dc.identifier.issn | 1008-682X | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12955/2172 | - |
dc.description.abstract | Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 °C (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results: When compared, the motility after thawing was higher (P < 0.05) in groups II-EG (20.0 % ± 6.7 %) and II-G (15.3 % ± 4.1 %) than that in groups I-G (4.0 % ± 1.1 %) and I-EG (1.0 % ± 1.4 %). Viable spermatozoa with intact acrosomes in groups II-EG (18.7 % ± 2.9 %) and II-G (12.7 % ± 5.9 %) were higher than that in groups I-G (5.7 % ± 1.5 %) and I-EG (4.0 % ± 1.0 %). Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders. | en |
dc.format | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wolters Kluwer Medknow Publications | en |
dc.relation.ispartof | Asian Journal of Andrology | en |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/3.0/ | - |
dc.source | Instituto Nacional de Innovación Agraria | es_PE |
dc.source.uri | Repositorio Institucional - INIA | es_PE |
dc.subject | Cryopreservation | en |
dc.subject | Glycerol | en |
dc.subject | Ethylene glycol | en |
dc.subject | Extenders | en |
dc.subject | Alpaca spermatozoa | en |
dc.title | Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders | en |
dc.type | info:eu-repo/semantics/article | - |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#4.02.01 | - |
dc.publisher.country | IN | - |
dc.identifier.doi | https://doi.org/10.1111/j.1745-7262.2005.00021.x | - |
dc.subject.agrovoc | Cryopreservation | en |
dc.subject.agrovoc | Criopreservación | en |
dc.subject.agrovoc | Ethylene glicol | en |
dc.subject.agrovoc | Etilenoglicol | en |
dc.subject.agrovoc | Alpacas | en |
dc.subject.agrovoc | Alpaca | en |
Aparece en las colecciones: | Artículos científicos |
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Santiani_et-al_2005_cryopreservation_alpacas.pdf | Article (English) | 281,4 kB | Adobe PDF | Visualizar/Abrir |
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