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dc.contributor.authorMarín, Alan-
dc.contributor.authorAlfaro, Ruben-
dc.contributor.authorVillegas Llerena, Claudio-
dc.contributor.authorReyes Flores, Lorenzo E.-
dc.contributor.authorAlvarez Jaque, Irina B.-
dc.contributor.authorRobles, Christian-
dc.contributor.authorIngar, Claudia-
dc.contributor.authorYzásiga Barrera, Carmen G.-
dc.contributor.authorCalado, Leonardo L.-
dc.contributor.authorZelada Mázmela, Eliana-
dc.coverage.spatialPerúes_PE
dc.date.accessioned2021-10-01T22:24:17Z-
dc.date.available2021-10-01T22:24:17Z-
dc.date.issued2021-06-12-
dc.identifier.citationMarín, A.; Alfaro, R.; Villegas, C.; Reyes, L.; Alvarez, I.; Robles, C.; Ingar, C.; Yzásiga, C.; Calado, L. & Zelada, E. (2021). Molecular tools against the illegal exploitation of the threatened Pacific seahorse Hippocampus ingens Girard, 1858. Journal for Nature Conservation 62, 126030. doi: 10.1016/j.jnc.2021.126030es_PE
dc.identifier.urihttps://hdl.handle.net/20.500.12955/1480-
dc.description.abstractThe high demand for syngnathid species in the traditional Chinese medicine (TCM) trade is drastically affecting seahorse populations worldwide. Powdered seahorse not only represents the main material for the preparation of remedies in either TCM or folk medicine but is also being used to facilitate smuggling and avoid customs authorities in different countries. Here, we present the first primer set and standardized real time quantitative PCR (qPCR) protocol designed for a fast, accurate, sensitive, and reliable identification of one of the most threatened fish species in the world, the Pacific seahorse Hippocampus ingens, using DNA traces from powdered material. Our protocol efficiently detected quantities as low as 0.85 pg of seahorse DNA, showing a high specificity even in the presence of DNA from other non-target fish species. The qPCR protocol reported herein may be utilized as a powerful tool to monitor the illegal domestic trade and international trafficking of H. ingens, not only in poaching hotspot countries such as Ecuador and Peru but also in transit and final market destinations such as Hong Kong and China. In addition, we show photographic evidence of seahorse extraction and trade in northern Peru and southern Ecuador, highlighting the need for increased efforts to protect the Pacific seahorse.es_PE
dc.description.tableofcontentsABSTRACT. 1. Introduction. 2. Materials and methods. 3. Results and discusión. 4. Conservation concerns. 5. Conclusions and recommendations. Referenceses_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherELSEVIERes_PE
dc.relation.ispartofJournal for Nature Conservation 62 (2021) 126030es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/es_PE
dc.sourceInstituto Nacional de Innovación Agrariaes_PE
dc.source.uriRepositorio Institucional - INIAes_PE
dc.subjectSeahorsees_PE
dc.subjectMitochondriales_PE
dc.subjectPoachinges_PE
dc.subjectWildlifees_PE
dc.subjectPerues_PE
dc.subjectTraditional Chinese medicinees_PE
dc.titleMolecular tools against the illegal exploitation of the threatened Pacific seahorse Hippocampus ingens Girard, 1858es_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.subject.ocdehttps://purl.org/pe-repo/ocde/ford#4.05.00es_PE
dc.identifier.journalJournal for Nature Conservationes_PE
dc.relation.publisherversionhttps://doi.org/10.1016/j.jnc.2021.126030es_PE
dc.publisher.countrySuizaes_PE
dc.identifier.doihttps://doi.org/10.1016/j.jnc.2021.126030-
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