Examinando por Materia "Strains"

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In vitro biological activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)
(Hindawi, 2023-09-30) Chuquibala Checan, Beimer; Torres de la Cruz, Magdiel; Leiva, Santos; Hernandez Diaz, Elgar; Rubio, Karol; Goñas Goñas, Malluri; Arce Inga, Marielita; Oliva Cruz, Manuel
Coffee (Coffea arabica) is the main commodity in Peru and is the economic support for thousands of small farmers. However, coffee production is affected by the coffee berry borer (Hypothenemus hampei). Currently, H. hampei is the most important pest in whole coffee-growing regions in Peru. This study aimed to evaluate in vitro biological activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei in two trials at different times. Conidia production, Conidia viability, and pathogenicity against H. hampei were evaluated at three concentrations (1 × 105, 1 × 107, and 1 × 109 conidia/mL−1). In addition, lethal times (LT50 and LT90) and lethal concentrations (LC50 and LC90) were calculated. There were significant differences in conidia production ( < 0.001) and conidia viability ( < 0.041). The highest conidia production and conidia viability were reached by B. bassiana and B. peruviensis, respectively. Likewise, there were differences in the pathogenicity of the strains in the two tests carried out (test 1: < 0.0009 and test 2: < 0.0001). The highest mortality occurred in the treatments of B. bassiana 1 × 109 conidia/mL−1, B. bassiana 1 × 107 conidia/mL−1, and B. peruviensis 1 × 109 conidia/mL−1. The treatments with lower LT50 and LT90 were B. bassiana 1 × 109 conidia/mL−1 and B. peruviensis 1 × 109 conidia/mL−1, and the strains with the lowest LC50 and LC90 were B. peruviensis and B. bassiana. The in vitro characteristics shown by B. bassiana and B. peruviensis conditions suggest they should be evaluated in the field to determine the capability of these strains to reduce populations of H. hampei.
The phylogeography of potato virus X shows the fingerprints of its human vector
(MDPI, 2021-09-09) Fuentes, Segundo; Gibbs, Adrian J.; Hajizadeh, Mohammad; Perez, Ana; Adams, Ian Patrick; Fribourg, Cesar E.; Kreuze, Jan; Fox, Adrian; Boonham, Neil; Jones, Roger A. C.
Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop′s main domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes plus 49 published genomic sequences were analyzed. Only 18 of them were recombinants, 17 of them Peruvian. A phylogeny of the non-recombinant sequences found two major (I, II) and five minor (I-1, I-2, II-1, II-2, II-3) phylogroups, which included 12 statistically supported clusters. Analysis of 488 coat protein (CP) gene sequences, including 128 published previously, gave a completely congruent phylogeny. Among the minor phylogroups, I-2 and II-3 only contained Andean isolates, I-1 and II-2 were of both Andean and other isolates, but all of the three II-1 isolates were European. I-1, I-2, II-1 and II-2 all contained biologically typed isolates. Population genetic and dating analyses indicated that PVX emerged after potato’s domestication 9000 years ago and was transported to Europe after the 15th century. Major clusters A–D probably resulted from expansions that occurred soon after the potato late-blight pandemic of the mid-19th century. Genetic comparisons of the PVX populations of different Peruvian Departments found similarities between those linked by local transport of seed potato tubers for summer rain-watered highland crops, and those linked to winter-irrigated crops in nearby coastal Departments. Comparisons also showed that, although the Andean PVX population was diverse and evolving neutrally, its spread to Europe and then elsewhere involved population expansion. PVX forms a basal Potexvirus genus lineage but its immediate progenitor is unknown. Establishing whether PVX′s entirely Andean phylogroups I-2 and II-3 and its Andean recombinants threaten potato production elsewhere requires future biological studies.