Examinando por Materia "Rizosfera"
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Ítem Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings(John Wiley & Sons Inc., 2024-07-17) Solórzano Acosta, Richard Andi; Quispe Matos, Kenyi RolandoThis research aims to assess the efficacy of two genera of rhizobacteria from avocado field isolated: Pseudomonas and Bacillus, as plant growth‐promoting microorganisms in Hass avocado trees grafted onto Zutano rootstock.Ítem Changes in bulk and rhizosphere soil microbial diversity communities of native quinoa due to the monocropping in the Peruvian Central Andes(MDPI, 2023-07-28) Estrada Cañari, Richard; Cosme de la Cruz, Roberto Carlos; Porras Valencia, Angie Tatiana; Reynoso Zárate, Auristela Florencia; Calderon, Constatino; Arbizu Berrocal, Carlos Irvin; Arone Gaspar, Gregorio JoseQuinoa (Chenopodium quinoa) is a highly nutritious crop that is resistant to adverse conditions. Due to the considerable increase in its commercial production in Andean soils, the plant is suffering the negative effects of monocropping, which reduces its yield. We used for the first time a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the bulk and rhizosphere in soils of native C. quinoa affected by monocropping in the central Andes of Peru. The results showed that the bacterial and fungal community structure among the treatments was significantly changed by the monocropping and the types of soil (rhizosphere and bulk). Also, in soils subjected to monocropping, there was an increase in Actinobacteria and a decrease in Proteobacteria, and the reduction in the presence of Ascomycota and the increase in Basidiomycota. By alpha-diversity indices, lower values of bacteria and fungi were observed in the monoculture option compared to the soil not affected by monocropping, and sometimes significant differences were found between both. We detected differentially abundant phytopathogenic fungi and bacteria with growth-stimulating effects on plants. Also, we denoted a decrease in the abundance of the functional predictions in bacteria in the monocropped soils. This research will serve as a starting point to explore the importance and effects of microorganisms in degraded soils and their impact on the growth and quality of quinoa crops.Ítem Metabarcoding reveals rhizosphere microbiome differences in healthy and basal rot-affected dragon fruit plants(Elsevier B.V., 2026-02-18) Guelac Santillan, Marly; Fernandez Castro, Paul; Huaman Pilco, Angel F.; Estrada Cañari, Richard; Rodríguez Grados, Pedro; Arbizu, Carlos I.The rhizosphere microbiome plays a crucial role in plant health, yet its dynamics in Selenicereus megalanthus (yellow dragon fruit) remain poorly understood. This study employed high-throughput sequencing to characterize the bacterial and fungal communities in the rhizosphere of healthy and basal rot-affected plants across four commercial production sites in Amazonas department from Peru. Amplicon sequencing Metagenomics Sequencing (WOBI) targeting to 16S rRNA (for bacteria) and ITS (for fungi) gene regions show differences in microbial community structure associated with plant health status. Multivariate analyses revealed a clear disease-driven reassembly of the bacterial microbiome, marked by the loss of health-associated taxa (Xanthobacteraceae, Geminicoccaceae, Nocardioidaceae) and enrichment of oligotrophic and stress-tolerant groups (Nitrososphaeraceae, Acidobacteriaceae Subgroup 1). In contrast, fungal assemblages displayed structural inertia, responding primarily through pathogen-associated increases in Nectriaceae. Soil physicochemistry particularly pH, exchangeable aluminum, and nutrient levels modulated the strength of bacterial differentiation, highlighting the role of edaphic filters in microbiome resilience. Our findings provide evidence of a bacterial-centered dysbiosis associated with basal stem rot in S. megalanthus, while positioning fungal communities as structurally resilient components of the holobiont. Together, these results outline a framework in which disease is linked to altered plant microbe soil feedbacks rather than pathogen presence alone, and suggest that bacterial assemblages could inform the development of microbiome-based early-warning indicators and soil health strategies for sustainable dragon fruit management.
