Examinando por Materia "Protein"

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Bioprocessing of organic wastes from poultry and bovine slaughterhouses as food substrate for Hermetia illucens larval development
(Global Journal of Environmental Science and Management, 2023-01-31) Luperdi, A. P.; Flores Calla, S. S.; Barriga, X. J.; Rivera, V.; Salazar, I.; Manrique, P. L.; Reátegui, J. E.
BACKGROUND AND OBJECTIVES: In the meat industry, inefficient management of organic waste exists, therefore the study aims to evaluate different bovine and poultry organic residues as food substrates during larval development of the black soldier fly, such as a sustainable alternative to obtain high protein meal. METHODS: The research evaluates the use of organic waste from cattle and poultry slaughterhouses, as food substrate for black soldier fly larvae, including raw beef blood T1, raw beef viscera T2, cooked beef blood T3, cooked beef viscera T4, raw chicken viscera T6 and cooked chicken viscera T7; further, as a control measure balanced feed (7 treatments and 5 replicates). Larvae were fed for 5 days and processed to make meal by drying and grinding; evaluating mortality, weight, size, proximal chemical composition, and apparent digestibility to determine the most viable substrate, analyzing effects and significance by multifactorial ANOVA and Kruskal-Wallis. FINDING: The results show Mortality (F = 917,81, p < 0,0001): T1 y T3 with 76,40 ± 2,86 (%) (F = 917,81, p < 0,0001), following T6 with 69,67 ± 4,55%, T7 with 24,00 ± 3,48%, T2 with 4,60 ± 1,92 %, T5 y T4, both with 4,20 ± 2,00 %. Weight (F = 825,62, p < 0,0001): T2 with 1,78 ± 0,22 gram outperformed the control T5 (1,76 ± 0,50 gram), T4 with 1,45 ± 0,06 g and T7 with 1,66 ± 0,07 gram. Size (F = 248,95, p < 0,0001): T5 with 16,03 ± 0,34 mm, T2 with 15,86 ± 0,22 mm, T4 with 14,72 ± 0,35 mm and finally, 14,51 ± 0,14 millimeter in T7. Proximal chemical analysis of crude protein and fat: T2 resulted in the following results 50,81 % and 21,88 %, T4 with 53,90% y 15,04%, T7 with 42,63 % and 32,03%, and T5 con 41,1 % and 19.55%, respectively. Digestibility: T5 with 20,39%, T2 with 12,66%, T4 with 10,61% and T7 with 5,97%. T2 raw beef viscera were determined to be the most viable substrate, followed by T4 cooked beef viscera and T7 cooked chicken viscera. CONCLUSIONS: Testing the effectiveness of cattle viscera as substrate, the experimental data presented may help design a process for an effective treatment method for slaughterhouse waste, which might benefit developing nations in managing their waste effectively, generating high protein meal, with the potential for a circular bioeconomy.
Effect of protein source and breed on embryo production in donor cows at a high-value genetic centre in Peru
(Unique Scientific Publishers, 2025-04-23) León Trinidad, Silvia Eliana; Dipaz Berrocal, Deisy Juana; Mamani, Rolando; Ramírez, Juan; Muñoz Vilchez, Yudith Yohany; Burga Toledo, Henry; Quilcate Pairazaman, Carlos Enrique; Alvarez García, Wuesley Yusmein
Two sources of concentrated animal protein at 50 and 60% of the diet of high genetic value cows were evaluated on embryo production in donor cows. Twenty Braunvieh (BU), Brahman (BR), Simmental (SM), Gir (G) and Girolando (GIHO) cows were evaluated for seven months. The SM breed achieved a higher number of corpora lutea, similar to Brahman cows, but no difference was shown between the two protein rations. It was determined that using both protein sources decreases production costs per feeding, with the same results in embryo production. This study emphasizes the great importance of adequate protein nutrition in embryo transfer programs to maximize reproductive efficiency and embryo viability, taking into account the welfare of the animals subjected to these reproductive evaluations, in order to improve productivity in a center for the production and reproduction of material or germplasm of high genetic value.
First characterization of somatic proteins of trematodes of the family Paramphistomidae by SDS PAGE isolated from cattle from the Cajamarca region, Peru
(Eldaghayes Publisher, 2026-01-31) Fernandez Mendoza, Charito Jennyfer; Tayca Saldaña, Antony; Cueva Rodríguez, Medali; Aliaga Tambo, Hector Fernando; Rodríguez Ulloa, Claudia Carolina; Alvarez García, Wuesley Yuesmein; Quilcate Pairazamán , Carlos Enrique; Cabrera González, Marco Antonio
ABSTRACT Background: Paramphistomosis is a gastrointestinal parasitic disease of worldwide distribution, with higher prevalence in tropical and subtropical regions. Its biological cycle has a chronic phase caused by adult trematodes that adhere to the rumen mucosa and a highly pathogenic phase caused by immature trematodes, which induce acute parasitic gastroenteritis. Studies related to the parasite are very scarce in the region of Cajamarca, Peru, regarding the characterisation of antigenic proteins, considering that this parasitosis is endemic and has emerging characteristics. Aim: The study aimed to characterise somatic proteins of adult forms of Paramphistomidae in cattle by electrophoresis, Sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Methods: For this purpose, 630 adult parasites were collected from the rumen and reticulum of cattle from the processing centre of Cajamarca, Peru. Results: The number of protein bands in the paramphistomid somatic crude extract was variable depending on the concentration of the protein analyzed. Thus, concentration 2 showed the highest number of protein bands (21 bands) with molecular weights (MW) within a variable range of 15 kDa–119 kDa. The results of ITS-2 gene sequencing, after alignment in NCBI’s Basic Local Alignment Search Tool of the obtained consensus sequences, show 100% similarity to sequences belonging to the species Calicophoron microbothrioides. Conclusion: Twenty-one protein bands from the somatic extract were characterized. The MWs of these bands ranged from 15 kDa to 119 kDa. This study may help carry out alternative control programmes, such as developing vaccines, choice of appropriate drugs due to the therapeutic failures expressed by this parasite. In addition, molecular identification based on ITS-2 gene sequencing revealed a 100% sequence similarity with Calicophoron microbothrioides.