Examinando por Materia "Potato"

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Draft Genome Sequence of Bacillus thuringiensis Strain UNMSM10RA, Isolated from Potato Crop Soil in Peru
(American Society for Microbiology, 2020-01-09) Delgado Silva, Yolanda Bedsabé; Tarazona, David; Serna Chumbes, Manuel Fernando; Juscamayta, Eduardo; Chávez Galarza, Julio César; Farfán Vignolo, Evelyn Roxana; Delgado, Gabriel; Flores, Abad; Solano, Gabriela; Gutiérrez Reynoso, Dina Lida
The 5.5-Mb genome sequence of Bacillus thuringiensis strain UNMSM10RA, isolated from potato crop soil, is reported in this study. The strain UNMSM10RA contains 5,347 protein-coding sequences, 105 tRNA genes, 15 rRNA genes, and 5 noncoding RNA (ncRNA) genes, with an average G+C content of 35.1%. Within protein-coding genes, 31 were detected and identified in the metabolism of heavy metals such as arsenic, cadmium, and copper. Further analyses will be performed and provide more information for understanding the evolution of these Bacillus thuringiensis strains and their potential uses.
Effect of production environment, genotype and process on the mineral content of native bitter potato cultivars converted into white chuño
(John Wiley & Sons, 2012-08-15) De Haan, Stef; Burgos, Gabriela; Ccanto, Raul; Arcos Pineda, Jesús; Scurrah, Maria; Salas Murrugarra, Elisa del Carmen; Bonierbale, Merideth
BACKGROUND: Variables and interaction effects affecting the mineral concentration of Andean bitter potatoes converted into so-called white chuño are unknown. We report on the effect of three contrasting production environments (E) on the dry matter (DM), zinc, iron, calcium, potassium, magnesium, phosphorus and sodium concentration of four potato native bitter genotypes (G) processed (P) into two different ‘types’ of white chuño. RESULTS: The DM content and iron, calcium, magnesium and sodium concentration of white chuño are significantly dependent on E, G, P, and E × G × P interaction (predominantly at P < 0.01). In particular, the DM content and calcium concentration are influenced by all variables and possible interaction effects. The zinc and potassium concentration are not significantly dependent on E × G, G × P or E × G × P interaction effects, while the phosphorus concentration is not significantly affected by the G × P or E × G × P interaction effect. Zinc, phosphorus and magnesium concentrations decrease in the ranges of 48.3-81.5%, 61.2-73.0% and 62.0-89.7% respectively. The decrease in potassium is particularly severe, with 122- to 330-fold losses. Iron and calcium increase by 11.2-45.6% and 74.5-714.9% respectively. CONCLUSION: E, G, P, and various interaction effects influence the mineral concentration of traditionally processed tubers. We speculate that mineral losses are caused by leaching, while increases of iron and calcium are a likely result of absorption.
Evaluating soil rhizobacteria for their ability to enhance plant growth and tuber yield in potato
(Wiley-Blackwell Publishing Ltd, 2010-08-17) Oswald, A; Calvo Velez, P.; Zúñiga Dávila, D.; Arcos Pineda, Jesus Heraclides
The objectives of this study were to identify promising microorganisms to improve potato productivity in low-input systems of tropical highlands and to compare results from in vitro, greenhouse and field experiments to advance the development of a screening method for rhizobacteria and develop an efficient assessment of their effect on plant growth in field conditions. A total of 150 bacterial strains were screened in vitro, in greenhouse and field trials. The series of experiments confirmed the plant growth-promoting ability of a range of rhizobacteria. Although in vitro and greenhouse results were promising, the field experiment showed variability and the results require further verification. The in vitro tests might have limited value for screening as no correlation could be found between in vitro tests and pot trial results. However, trials in controlled conditions produced insights into the mechanisms causing better plant growth in potato, such as early tuberisation, fast development of leaf area and probably greater photosynthetic rates.
Greatly reduced phylogenetic structure in the cultivated potato clade (Solanum section Petota pro parte)
(John Wiley & Sons, 2018-02-15) Spooner, David M.; Ruess, Holly; Arbizu Berrocal, Carlos Irvin; Rodríguez, Flor; Solís Lemus, Claudia
•Premise of the Study: osible ies boundaries of wild and cultivated potatoes are controversial, with osible the taxonomic problems in the cultivated potato clade. We here provide the first in‐depth phylogenetic study of the cultivated potato clade to explore osible causes of these problems. •Methods: We examined 131 diploid accessions, using 12 nuclear orthologs, producing an aligned data set of 14,072 DNA characters, 2171 of which are parsimony‐informative. We analyzed the data to produce phylogenies and perform concordance analysis and goodness‐of‐fit tests. •Key Results: There is good phylogenetic structure in clades traditionally referred to as clade 1+2 (North and Central American diploid potatoes exclusive of Solanum verrucosum), clade 3, and a newly discovered basal clade, but drastically reduced phylogenetic structure in clade 4, the cultivated potato clade. The results highlight a clade of species in South America not shown before, ‘neocardenasii’, sister to clade 1+2, that possesses key morphological traits typical of diploids in Mexico and Central America. Goodness‐of‐fit tests suggest potential hybridization between some species of the cultivated potato clade. However, we do not have enough phylogenetic signal with the data at hand to explicitly estimate such hybridization events with species networks methods. •Conclusions: We document the close relationships of many of the species in the cultivated potato clade, provide insight into the cause of their taxonomic problems, and support the recent reduction of species in this clade. The discovery of the neocardenasii clade forces a reevaluation of a hypothesis that section Petota originated in Mexico and Central America.
Identification of Elite Potato Clones with Resistance to Late Blight Through Participatory Varietal Selection in Peru
(European Association for Potato Research, 2021-06-12) Gastelo Benavides, Manuel Antonio; Burgos, Gabriela; Bastos Zuñiga, Carolina; Perez, Juan Miguel; Otiniano Villanueva, Ronal; Cabrera Hoyos, Héctor Antonio; Ccanto, Raul; De Scurrah, María Mayer; Zum Felde, Thomas
Potato is the most important crop in Peru and late blight is the main disease affecting the crop. However, new varieties that are resistant to late blight often lack other traits that farmers and consumers prefer. Using participatory varietal selection, this study seeks to identify clones with high potential to become varieties with resistance to late blight but also feature a high marketable tuber yield and other preferred agronomic traits. During 2016–2017, 36 clones previously selected for high levels of resistance to late blight from population B developed by the International Potato Center, and three varieties used as controls (INIA 302 Amarilis moderately resistant, INIA303 Canchan and Yungay susceptible to late blight), were evaluated in five Peruvian locations. At harvest, five clones were selected based on (i) evaluations made by farmers through Participatory Varietal Selection, (ii) analysis of mixed models and Best Linear Unbiased Predictors for tuber yield, (iii) low glycoalkaloid content in tubers, and (iv) good organoleptic quality. These clones were evaluated again during 2017–2018 in four locations. Resistance to late blight and good marketable tuber yields were identified as the most important criteria for the selection of a new potato variety. The clones CIP308488.92, CIP308495.227 and CIP308478.59 were selected as promising clones having resistance to late blight and tuber yield superior to the local varieties, INIA-303 Canchan, Yungay, as well as good organoleptic quality and low glycoalkaloid content. These clones can be suggested for variety release in similar agroecological environments.
Obtaining new potato cultivars with late blight resistance and adapted to climate change using participatory varietal selection
(David Publishing Company, 2020-02-28) Zúñiga López, Luz Noemí; Gastelo Benavides, Manuel Antonio; Bastos Zuñiga, Carolina; Reyes Espinoza, Jhercy Pepe; Alania, Edson; Ninalaya Cerrón, Evelin Natali
The potato crop in Peru is the main income and food source for the smallholder of the highland grower of the Peruvian Andes. For urban population, it represents one of the main components of the food basket and for commercial intermediaries. It is the main brokerage product considering its volume and value. To facilitate the adoption and diffusion of new potato cultivars after its release, adequate methodologies are necessary to apply during the process of evaluation and selection. One of these methodologies is called participatory varietal selection (PVS). The objective of this study was to identify new potato cultivars and/or genotypes with resistance or tolerance to adverse effects of climate change applying PVS, in order to obtain new varieties with acceptable economic yields. In this way potato growers of the Peruvian highland Andes should benefit through the improvement of their economic status, food security and overall life quality. During 2016-2017, three potato clones (CIP393079.4, CIP387096.2 and CIP396034.268) and two commercial varieties (Unica, Canchan) were evaluated at eight Peruvian locations in three regions (Huánuco, Junín and Huancavelica). Data were analyzed using a randomized complete block design, with three replications. Main criteria for selection were late blight resistance, abundant foliage, drought tolerance, yield and uniform and healthy tubers. In all three regions the three best clones selected were CIP396034.268, CIP393079.4 and CIP387096.2, ranking in first, second and third place, respectively. Men and women selected the same clones, but in a different order. Commercial cultivars ranked 4th and 5th. However, local growers selected Canchan, CIP387096.2 and CIP393079.4 based on appearance, texture and taste tests. The last two clones (CIP387096.2 and CIP393079.4) should be considered for a future release.
The phylogeography of potato virus X shows the fingerprints of its human vector
(MDPI, 2021-09-09) Fuentes, Segundo; Gibbs, Adrian J.; Hajizadeh, Mohammad; Perez, Ana; Adams, Ian P.; Fribourg, Cesar E.; Kreuze, Jan; Fox, Adrian; Boonham, Neil; Jones, Roger A. C.
Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop′s main domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes plus 49 published genomic sequences were analyzed. Only 18 of them were recombinants, 17 of them Peruvian. A phylogeny of the non-recombinant sequences found two major (I, II) and five minor (I-1, I-2, II-1, II-2, II-3) phylogroups, which included 12 statistically supported clusters. Analysis of 488 coat protein (CP) gene sequences, including 128 published previously, gave a completely congruent phylogeny. Among the minor phylogroups, I-2 and II-3 only contained Andean isolates, I-1 and II-2 were of both Andean and other isolates, but all of the three II-1 isolates were European. I-1, I-2, II-1 and II-2 all contained biologically typed isolates. Population genetic and dating analyses indicated that PVX emerged after potato’s domestication 9000 years ago and was transported to Europe after the 15th century. Major clusters A–D probably resulted from expansions that occurred soon after the potato late-blight pandemic of the mid-19th century. Genetic comparisons of the PVX populations of different Peruvian Departments found similarities between those linked by local transport of seed potato tubers for summer rain-watered highland crops, and those linked to winter-irrigated crops in nearby coastal Departments. Comparisons also showed that, although the Andean PVX population was diverse and evolving neutrally, its spread to Europe and then elsewhere involved population expansion. PVX forms a basal Potexvirus genus lineage but its immediate progenitor is unknown. Establishing whether PVX′s entirely Andean phylogroups I-2 and II-3 and its Andean recombinants threaten potato production elsewhere requires future biological studies.
Traditional potato tillage systems in the Peruvian Andes impact bacterial diversity, evenness, community composition, and functions in soil microbiomes
(Nature Publishing Group, 2024-02-17) García Serquén, Aura Liz; Chumbe Nolasco, Lenin Dimitriv; Navarrete, Acacio Aparecido; Girón Aguilar, Rita Carolina; Gutiérrez Reynoso, Dina Lida
The soil microbiome, a crucial component of agricultural ecosystems, plays a pivotal role in crop production and ecosystem functioning. However, its response to traditional tillage systems in potato cultivation in the Peruvian highlands is still far from understood. Here, ecological and functional aspects of the bacterial community were analyzed based on soil samples from two traditional tillage systems: 'chiwa' (minimal tillage) and 'barbecho' (full tillage), in the Huanuco region of the Peruvian central Andes. Similar soil bacterial community composition was shown for minimal tillage system, but it was heterogeneous for full tillage system. This soil bacterial community composition under full tillage system may be attributed to stochastic, and a more dynamic environment within this tillage system. 'Chiwa' and 'barbecho' soils harbored distinct bacterial genera into their communities, indicating their potential as bioindicators of traditional tillage effects. Functional analysis revealed common metabolic pathways in both tillage systems, with differences in anaerobic pathways in 'chiwa' and more diverse pathways in 'barbecho'. These findings open the possibilities to explore microbial bioindicators for minimal and full tillage systems, which are in relationship with healthy soil, and they can be used to propose adequate tillage systems for the sowing of potatoes in Peru.
Traditional processing of black and white chuño in the peruvian andes: regional variants and effect on the mineral content of native potato cultivars
(Springer Nature, 2010-08-11) De Haan, Stef; Burgos, Gabriela; Arcos Pineda, Jesús; Ccanto, Raul; Scurrah, Maria; Salas Murrugarra, Elisa del Carmen; Bonierbale, Merideth
Traditional Processing of Black and White Chuño in the Peruvian Andes: Regional Variants and Effect on the Mineral Content of Native Potato Cultivars. Farmers in the high Andes of central to southern Peru and Bolivia typically freeze-dry potatoes to obtain chuño. Processing of so-called black chuño follows tending, treading, freezing, and drying. The making of white chuño is generally more complex and involves exposure of tubers to water. Regional variants exist for each of these processes, yet their influence on the nutritional composition of native potato cultivars is little known. Tubers belonging to four distinct cultivars and produced in a replicated trial under uniform conditions were processed into four types of chuño following standard traditional procedures (farmer-managed). These regional variants were documented, and the dry matter, iron, zinc, calcium, potassium, phosphorus, magnesium, and sodium content of the four resulting different types of boiled chuño determined at the International Potato Center's Quality and Nutrition Laboratory (Lima, Peru). Content values were compared with those of boiled (unprocessed) tubers from the same experiment. Regional variants of processing are to a large extent determined by tradition, environmental condition, and market demand. The zinc, potassium, phosphorus, and magnesium content of all types of chuño decreases in comparison with unprocessed tubers. Concentrations of these same minerals decrease more drastically for white as compared to black chuño. The effect of the four regional variants of freeze-drying on the dry matter, iron, calcium, and sodium content of chuño differs by process and/or cultivar.