Examinando por Materia "PGPR"

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Assessing the role of field isolated Pseudomonas and Bacillus as growth‐promoting rizobacteria on avocado (Persea americana) seedlings
(John Wiley & Sons Inc., 2024-07-17) Solórzano Acosta, Richard Andi; Quispe Matos, Kenyi Rolando
This research aims to assess the efficacy of two genera of rhizobacteria from avocado field isolated: Pseudomonas and Bacillus, as plant growth‐promoting microorganisms in Hass avocado trees grafted onto Zutano rootstock.
Co-inoculation with Rhizobium leguminosarum bv. viciae and Azospirillum brasilense outperforms nitrogen fertilisation in terms of yield, crude protein content, and profitability of green pea (Pisum sativum L.) cultivation in the Peruvian Andes
(Frontiers Media S.A., 2026-08-20) Bellido Tenorio, Enrique; Alarcón Romani, Susan Milagros; Condori Ataupillco, Leivi Tatiana; Palomino-Espinoza, Claudio Carlos; Rengifo Sanchez, Raihil Rabindranath; Erazo Huarache, José Manuel; García Blásquez, Cayo; Solórzano Acosta, Richard Andi
Green pea (Pisum sativum L.) is a key crop for food security in communities of the Peruvian Andes; however, its productivity is constrained by low soil fertility and the high cost of nitrogen fertilisers. In this context, the effects of co-inoculation with Rhizobium leguminosarum bv. viciae (strain Ar-014) and Azospirillum brasilense (strain Az-007) on crop yield, crude protein content, and economic profitability were evaluated in comparison with conventional nitrogen fertilisation. The study was conducted at the Canaan Agrarian Experimental Station (Ayacucho, Peru; 2,750 m.a.s.l.) using a randomised complete block design with five treatments and four replicates: T01 (Rhizobium leguminosarum bv. viciae inoculation), T02 (Azospirillum brasilense inoculation), T03 (co-inoculation), T04 (mineral-N comparator, 82.5 kg N ha⁻¹), and T05 (uninoculated and unfertilised control). The results showed that T03 (co-inoculation) significantly outperformed nitrogen fertilisation (T04) (p < 0.01), achieving higher green pod yield (6.73 vs 5.63 t ha⁻¹), crude protein content (24.28 vs 23.67%), and economic profitability (262.75 vs 176.78%). Furthermore, T03 recorded the highest values for root length (21.73 cm), number of nodules per plant (20.27), and number of seeds per pod (6.07). Individual inoculation treatments (T01 and T02) also improved specific agronomic traits, such as aboveground biomass and number of pods per plant, respectively, although neither surpassed the performance of the co-inoculation treatment (T03). The Relative Yield Index (RYI_N) in the inoculated treatments was 16.85–19.49% higher than that observed under nitrogen fertilization. Overall, these results indicate that co-inoculation with R. leguminosarum b. viciae and A. brasilense (T03) represents a promising alternative to conventional nitrogen fertilization for green pea production under the evaluated high-Andean conditions, improving agronomic performance and economic profitability.
Co-inoculation with Rhizobium leguminosarum bv. viciae and Azospirillum brasilense outperforms nitrogen fertilisation in terms of yield, crude protein content, and profitability of green pea (Pisum sativum L.) cultivation in the Peruvian Andes
(Frontiers Media S.A., 2026-09-17) Bellido Tenorio, Enrique; Alarcón Romani, Susan Milagros; Condori Ataupillco, Leivi Tatiana; Palomino Espinoza, Claudio Carlos; Rengifo Sanchez, Raihil Rabindranath; Erazo Huarache, José Manuel; García Blásquez, Cayo; Solórzano Acosta, Richard Andi
Green pea (Pisum sativum L.) is a key crop for food security in communities of the Peruvian Andes; however, its productivity is constrained by low soil fertility and the high cost of nitrogen fertilisers. In this context, the effects of co-inoculation with Rhizobium leguminosarum bv. viciae (strain Ar-014) and Azospirillum brasilense (strain Az-007) on crop yield, crude protein content, and economic profitability were evaluated in comparison with conventional nitrogen fertilisation. The study was conducted at the Canaan Agrarian Experimental Station (Ayacucho, Peru; 2,750 m.a.s.l.) using a randomised complete block design with five treatments and four replicates: T01 (Rhizobium leguminosarum bv. viciae inoculation), T02 (Azospirillum brasilense inoculation), T03 (co-inoculation), T04 (mineral-N comparator, 82.5 kg N ha−1), and T05 (uninoculated and unfertilised control). The results showed that T03 (co-inoculation) significantly outperformed nitrogen fertilisation (T04) (p < 0.01), achieving higher green pod yield (6.73 vs. 5.63 t ha−1), crude protein content (24.28 vs. 23.67%), and economic profitability (262.75 vs. 176.78%). Furthermore, T03 recorded the highest values for root length (21.73 cm), number of nodules per plant (20.27), and number of seeds per pod (6.07). Individual inoculation treatments (T01 and T02) also improved specific agronomic traits, such as aboveground biomass and number of pods per plant, respectively, although neither surpassed the performance of the co-inoculation treatment (T03). The Relative Yield Index (RYI_N) in the inoculated treatments was 16.85–19.49% higher than that observed under nitrogen fertilization. Overall, these results indicate that co-inoculation with R. leguminosarum b. viciae and A. brasilense (T03) represents a promising alternative to conventional nitrogen fertilization for green pea production under the evaluated high-Andean conditions, improving agronomic performance and economic profitability.
Inoculation with Azospirillum brasilense as a strategy to reduce nitrogen fertilization in cultivating purple maize (Zea mays l.) in the Inter-andean valleys of Peru
(MDPI, 2024-10-21) Condori Ataupillco, Tatiana; Alarcón Romani, Susan; Huasasquiche Sarmiento, Lucero; García Blásquez, Cayo; Padilla Castro, César; Velásquez Mantari, José; Solórzano Acosta, Richard Andi
Purple maize has gained global significance due to its numerous nutraceutical benefits. However, sustaining its production typically requires high doses of nitrogen fertilizers, which, when applied in excess, can contaminate vital resources such as soil and water. Inoculation with nitrogenfixing microorganisms, such as those from the Azospirillum genus, has emerged as an alternative to partially or fully replace nitrogen fertilizers. This study aimed to evaluate the inoculation effect with A. brasilense and varying nitrogen fertilization levels on the yield and quality of purple maize. The experiment was carried out using a randomized complete block design (RCBD) with a 2 × 5 factorial arrangement and five replications. Treatments comprised two inoculation levels (control without inoculation and inoculation with A. brasilense) under five nitrogen doses (0, 30, 60, 90, and 120 kg・ha−1, applied as urea). Inoculation with A. brasilense resulted in a 10.5% increase in plant height, a 16.7% increase in root length, a 21.3% increase in aboveground fresh biomass, a 30.1% increase in root fresh biomass, and a 27.7% increase in leaf nitrogen concentration compared to the no inoculated control. Regarding yield, the inoculated plants surpassed the control in both purple maize yield (kg・ha−1) and cob weight by 21.8% and 11.6%, respectively. Across all fertilization levels and parameters assessed, the inoculated treatments outperformed the control. Furthermore, for parameters, namely plant height, leaf nitrogen content, and cob dimensions (length, diameter, and weight), the A. brasilense inoculation treatment with 90 kg N・ha−1 was statistically equivalent or superior to the non-inoculated control with 120 kg N・ha−1. These results indicate that inoculation with A. brasilense positively impacted purple maize at all nitrogen levels tested and improved nitrogen use efficiency, enabling a reduction of 30 kg N・ha-1 without compromising performance in key parameters.
Molecular analysis of the diversity of nitrogen-fixing and phosphate-solubilizing bacteria in Neltuma pallida (Humb. & Bonpl. ex Willd.) Kunth
(Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, 2026-08-17) García León, Mack Frank; Córdova Campos, José Stalyn; Garcia Garcia, Segundo Melecio; Ruiz Polo, Archi Alejandro; Mialhe Matonnier, Eric Louis; García Seminario, Ramón; Mogollón Farias, César Augusto
La identificación de bacterias fijadoras de nitrógeno y solubilizadoras de fosfato atmosféricas emerge como una alternativa de fertilización sostenible al uso de agroquímicos que contaminan el medio ambiente. El objetivo de esta investigación fue analizar molecularmente la diversidad de bacterias fijadoras de nitrógeno y solubilizadoras de fosfato en Neltuma pallida. Se recolectaron muestras de suelo rizosférico de árboles jóvenes de Neltuma pallida ubicados en el campo de prácticas del programa de Ingeniería Forestal y Ambiental de la Universidad Nacional de Tumbes, en la localidad de Garbanzal. Las bacterias se aislaron del suelo, se caracterizaron morfológicamente y se evaluaron en ensayos de fijación de nitrógeno inoculando 5 µL de suspensiones bacterianas (1×10⁸ UFC/mL) por triplicado en medio de cultivo ASHBY. Posteriormente, se seleccionaron los aislados con mayor fijación de nitrógeno para ensayos de solubilización de fosfato, los cuales se inocularon en medio NBRIP suplementado con azul de bromofenol (25 ppm), utilizando la misma concentración bacteriana. El análisis de datos se realizó con el software R Studio. Finalmente, se realizó la identificación de los aislados mediante la secuenciación del gen 16S rRNA y se establecieron las relaciones evolutivas a través de un árbol filogenético. En total, se aislaron 50 cepas bacterianas, de las cuales 41 tenían la capacidad de fijar nitrógeno atmosférico. Entre estas, 10 destacaron por su alta eficiencia de fijación y, de estas, cinco también mostraron la capacidad de solubilizar fosfato. Sin embargo, solo dos cepas exhibieron alta eficiencia en ambas funciones. Las relaciones filogenéticas agruparon las especies en tres clados (Firmicutes, Proteobacteria y Bacteroidetes). Las especies identificadas con doble capacidad (fijación de nitrógeno y solubilización de fosfato) fueron Bacillus megaterium, Erwinia spp., Bacillus flexus, Bacillus amyloliquefaciens y Pantoea spp., mientras que aquellas que solo fijaban nitrógeno incluían Bacillus endophyticus, Olivibacter spp., Pantoea septica y Lysinibacillus xylanilyticus.