Examinando por Materia "Microbial inoculants"

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Bacillus subtilis and Rhizophagus intraradices improve vegetative growth, yield, and fruit quality of Fragaria × ananassa var. San Andreas
(MDPI, 2024-09-02) Huasasquiche Sarmiento, Lucero; Alejandro, Leonela; Ccori, Thania; Cántaro Segura, Héctor; Samaniego Vivanco, Tomás Daniel; Quispe Matos, Kenyi Rolando; Solórzano Acosta, Richard Andi
Strawberry cultivation requires strategies that maintain or improve its yield within a scheme in which reducing fertilizers and other chemical products can make its consumption safer and more environmentally friendly. This study aims to evaluate the effect of Bacillus subtilis and Rhizophagus intraradices on strawberry growth, yield, and fruit quality. B. subtilis and R. intraradices were inoculated and co-inoculated under three fertilization levels of 225-100-250, 112-50-125, and 0-0-0 kg・ha−1 of N, P2O5 and K2O. Vegetative growth was evaluated in plant height (cm), leaf area (cm2), aerial fresh weight (g), aerial dry weight (g), and plant coverage (%) variables. Fruit quality parameters such as total acidity (g・100 mL−1), soluble solids (Brix◦), and firmness (kg) were also determined, as well as the number of fruits per m2 and yield (t・ha−1). The results showed that the pre-treatment of root immersion in a nutrient solution with B. subtilis and the fractionation of 6 L B. subtilis inoculation per plant at a concentration of 107 CFU・mL−1, in combination with 225-100-250 kg・ha−1 of N, P2O5, and K2O, achieved the highest accumulation of dry matter (12.9 +- 1.9 g・plant−1), the highest number of fruits (28.2 +- 4.5 fruits・m−2), and the highest yield (7.2 +- 1.4 t・ha−1). In addition, this treatment increased the soluble sugar content by 34.78% and fruit firmness by 26.54% compared to the control without inoculation. This study highlights the synergistic effect of mineral nutrition and microbial inoculation with B. subtilis in increasing strawberry yield and fruit quality.
Interaction between Trichoderma sp., Pseudomonas putida, and two organic amendments on the yield and quality of strawberries (Fragaria x annanasa cv. San Andreas) in the Huaral region, Peru
(MDPI, 2024-07-22) Huasasquiche, Lucero; Ccori, Thania; Alejandro, Leonela; Cántaro Segura, Héctor; Samaniego, Tomás; Solórzano, Richard
Strawberry cultivation holds significant economic and social promise within Peruvian fruit production. However, conventional management practices have led to the excessive use of agrochemicals in this crop. This study proposes an organic approach to strawberry production, integrating less environmentally harmful technologies. The aim was to assess microbial inoculation by using Trichoderma sp. and Pseudomonas putida and the application of organic amendments on strawberry seedlings of the commercial cultivar “San Andreas”. A field experiment was established with evaluations in the vegetative and productive stages. Results indicate that the co-inoculation of Trichoderma sp. and Pseudomonas putida increased leaf area by 7%, and enhanced the aerial part’s fresh and dry biomass by 13% and 28%, respectively, compared to treatment without microbial inoculation. Concurrently, compost application increased the leaf number and aerial dry biomass by 22% and 19% at the end of the vegetative stage, respectively, compared to treatment without organic amendment. In addition, it reduced the days for flowering, maintaining the fruit’s physicochemical attributes. Regarding yield, the amendments application significantly enhanced fruit weight per plant by 40%, especially when applied together with Trichoderma sp., and co-inoculation increased the number of fruits per meter square by 22%. These findings highlight the potential of technologies such as microbial inoculation and organic amendments to enhance strawberry yields and to gradually reduce the use of synthetic fertilizers.
Multistage evaluation of plant growth-promoting microorganisms in Leucaena leucocephala and Megathyrsus maximus cv. Mombasa: controlled experiments and preliminary field observations in northern Peru
(MDPI, 2026-09-01) Romero Avila, Yolanda Madelein; Muñóz Rabanal, Elías; Valladolid, Fabian; Castro Angulo, Raúl Ericson; Casas Niño, Sebastian Antonio; Ruiz Chamorro, Jose Antonio; Cruz Luis, Juancarlos Alejandro
Plant growth-promoting microorganisms (PGPM) may contribute to sustainable forage production, but their effects can vary with host species and plant trait. Bacillus subtilis, Pseudomonas putida, Trichoderma harzianum, and Trichoderma viride were evaluated alongside a non-inoculated control in Leucaena leucocephala and Megathyrsus maximus cv. Mombasa in northern Peru. Replicated germination and nursery experiments were complemented by a preliminary field assessment. Inoculation did not significantly affect final germination percentage in either forage species. In the nursery, L. leucocephala exhibited significant inoculant treatment effects on multiple growth traits, including positive responses in plant height and leaf number under B. subtilis, P. putida, and T. harzianum at the final evaluation. Significant treatment effects were also detected for several final aboveground and belowground growth traits, whereas total weight was not significantly affected. By contrast, growth variation in M. maximus cv. Mombasa was mainly associated with evaluation time, while microbial treatment and treatment × time effects were not significant. Multivariate analysis detected significant treatment and species × treatment effects, indicating contrasting joint growth-response profiles between the two forage species without demonstrating specificity of individual host–microorganism combinations. Preliminary field measurements showed numerical variation among treatment-associated rows, but these observations were descriptive only because treatments were neither randomized nor independently replicated. Overall, this comparative evaluation shows that PGPM responses in tropical forage species can be strongly species- and trait-dependent under nursery conditions, while validation of these responses under field conditions requires appropriately randomized and independently replicated experiments.