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Ítem Inoculation with Trichoderma in Coffea arabica seedlings: Effects on morphological indices and seedling quality under nursery conditions(MDPI, 2026-08-04) Camacho Villalobos, Alina Alexandra; Amaringo Córdova, Luiz Paulo; Ucañay Ayllon, Tatiana Mildred; Salgado Veramendi, Noelito; Flores Jaramillo, Jhoffre David; Aldava Pardave, UrielMicrobial inoculants such as Trichoderma have been proposed as sustainable tools to improve seedling quality in coffee nurseries; however, their effectiveness may vary according to formulation and genotype. This study evaluated three treatments: a solid Trichoderma formulation (1 kg m−3 of substrate) based on a multispecies consortium (T. harzianum, T. asperellum, and T. viride; 5 × 109 conidia g−1), a liquid formulation of T. harzianum (1 × 108 CFU mL−1; 1 L m−3 of substrate), and a non-inoculated control, using three Coffea arabica varieties (Catimor, Marsellesa, and Gran Colombia) under controlled nursery conditions. A linear mixed model was applied, considering variety and inoculant type as fixed effects and replicates as random effects. The results show that the biostimulant response is strongly modulated by the variety × inoculant interaction. The solid formulation was superior in promoting the expansion of the photosynthetic apparatus, increasing leaf area by 24.6%. In turn, the liquid formulation optimized structural vigor, increasing stem diameter by 17.3% and reducing slenderness by 13.1%. Inoculation reduced the lignification index by 10.4%, favoring dynamic vegetative growth. These improvements were integrated into the Dickson Quality Index, which reached values of 0.41–0.70, greatly exceeding the critical threshold of 0.20 for medium- to high-quality seedlings. It is concluded that Trichoderma acts as a physiological compensator that harmonizes plant architecture, although its efficacy depends on specific compatibility with the genotype, which is essential for ensuring the successful establishment of coffee plants under field conditions.Ítem Nursery management modulates biomass allocation and early field establishment of Pinus douglasiana Martínez(MDPI, 2026-08-20) Bernaola Paucar, Rosario Marilu; Ruiz Blandon, Bayron Alexander; Alama Flores, Marcos; Amaringo Córdova, Luiz Paulo; Rodríguez Cangalaya, Nora; Colachagua Calderon, Deysi Alina; Nieto Ramos, Carlos Emérico; Nieto Ramos, Luis Armando; Cuadrado Campó, Walter JavierNursery decisions influence the condition of seedlings before outplanting, but their practical value depends on whether those traits remain evident during early field establishment. We evaluated Pinus douglasiana Martínez seedlings grown under eight combinations of container volume, fertilization, and irrigation regime. Seedlings were conditioned for six months in the nursery and then assessed after 12 months under field conditions. Container volume was the clearest source of variation. Compared with 1 L containers, 5 L containers produced seedlings with larger root collar diameter, greater shoot, root, and total biomass, and higher Dickson quality index. Total biomass was 2.2-fold greater in 5 L stock. Final survival was retained as a descriptive field variable and averaged 76.7% in 5 L treatments and 27.5% in 1 L treatments. Correlations based on treatment means associated total biomass with field diameter and descriptive survival, although these patterns were exploratory because only eight treatment means were available. Proline increased mainly under non-continuous irrigation, indicating a water-related nursery response, but it was not aligned with biomass production or first-year field performance. The treatment-level PCA separated biomass and field-growth variables from proline and lignification. These results show that larger containers improved the structural condition of P. douglasiana planting stock, although nursery costs, planting-site constraints, and longer monitoring should be considered before operational adoption.
