Examinando por Materia "Lolium multiflorum"

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Genomic insights into Lolium multiflorum diversity for forage breeding in Andean livestock systems
(Frontiers Media S.A., 2026-06-10) Bobadilla , Leidy G.; Ortiz, Rodomiro; Castillo, Gianmarco; Matías, Felipe; Gutiérrez, Lucía; Valqui, Leandro; Carrasco Chilón, William Leoncio; Díaz Valderrama, Jorge R.; Venancio, Thiago M.; Alava, E.I.; Castillo, Miguel S.; Vásquez, Héctor V.
Understanding the genetic diversity of Lolium multiflorum is essential for developing targeted breeding programs that can effectively support pasture-based livestock systems in the Peruvian Andean. This study assessed genomic variability and population structure in 27 L. multiflorum accessions from the Cajamarca region and the INIA Amazonas germplasm bank (Peru), using the genotyping-by-sequencing (GBS) technique. DNA extracted from young leaves was sequenced on an Illumina NovaSeq 6000 platform. After bioinformatic processing, 2,070 single nucleotide polymorphisms (SNPs) with heterogeneous distribution were obtained across seven chromosomes. A Principal Coordinate and an Unweighted Pair Group Method with Arithmetic Mean analyses revealed two distinct genetic groups were identified, reflecting a complex structure shaped by gene flow and local selection. The analysis of molecular variance showed that 90% of the genetic variation occurs within populations, whereas the remaining 10% corresponds to interregional differences (PhiPT = 0.099, p < 0.006). The negative Inbreeding Coefficient (F_IS) values (Cajamarca = -0.2312; Amazonas = -0.5489) indicate an excess of heterozygotes, a pattern typically associated with predominantly outcrossing species. Additionally, high observed heterozygosity (Ho > 0.57) points to potential hybrid vigor and indicates that these populations may maintain stable genetic equilibrium. Collectively, these findings demonstrate that Peruvian L. multiflorum harbors a broad genetic base, shaped by historical germplasm exchange and local environmental adaptation. This diversity provides critical insights for conservation strategies and can supports breeding programs aimed at enhancing forage resilience and productivity in high-Andean ecosystems.
Morphological differentiation, yield, and cutting time of Lolium multiflorum L. under acid soil conditions in Highlands
(MDPI, 2024-08-21) Carrasco Chilón, William; Cervantes Peralta, Marieta; Mendoza, Laura; Muñoz Vílchez, Yudith; Quilcate, Carlos; Casanova Nuñez Melgar, David; Vásquez, Héctor; Alvarez García, Wuesley Yusmein
Livestock production in the basins of the northern macro-region of Peru has as its primary source pastures of Lolium multiflorum L. ‘Cajamarquino ecotype’ (ryegrass CE) in monoculture, or in association with white clover Ladino variety, for feeding. The objective of this research work was the morphological characterisation, yield evaluation, and cutting time evaluation of two local genotypes (LM-58 and LM-43) of Lolium multiflorum L. in six locations. An ANOVA was performed to compare fixed effects and interaction. It was determined that the LM-58 genotype is intermediate, growing semi-erect, with a dark green colouring and 0.8 cm broadleaf, and can reach an average stem length of 46 cm, up to 1.6 cm. day−1, achieving fourth-leaf growth at 28 days under appropriate management conditions. Despite the differentiated characteristics, according to BLASTn evaluation, the ITS1 sequences showed a greater than 99.9% similar identification to Lolium multiflorum L., characterising it as such. It was determined that the LM-58 genotype outperforms LM-43, achieving a forage yield of 4.49 Mg. ha−1, a seed production of 259.23 kg. ha−1, and an average of 13.48% crude protein (CP). The best biomass yield (49.10 Mg. ha−1.yr−1) is reached at 60 days; however, at 30 days, there is a high level of CP (14.84%) and there are no differences in the annual protein production at the cutting age of 60 and 45 days. With the results of the present study, LM-58 from a selection and crossbreeding of 680 ryegrass EC accessions emerges as an elite genotype adapted to the conditions of the northern high Andean zone of Peru.