Examinando por Materia "Genetics"

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Inter-primer binding site (iPBS) retrotransposon markers provide insights into the genetic diversity and population structure of carrots (Daucus, Apiaceae)
(TUBITAK, 2022-04-25) Yildiz, Mehtap; Arbizu Berrocal, Carlos Irvin
Studies of genetic diversity and population structure are essential as an initial step in conservation and breeding programs for modern crops. Carrot (Daucus spp.) is among the ten most important vegetables worldwide, however, its genetic structure and phylogenetic relationships are not totally deciphered. Here, we explored the utility of 21 inter-primer binding site (iPBS) retrotransposon markers to determine the genetic diversity and population structure of 38 accessions of Daucus and one accession of a related genus. The manual scoring revealed 309 bands based on their presence/absence. The dendrogram based on the UPGMA clustering algorithm and a principal coordinate analysis (PCoA) indicated the presence of four clusters. The Daucus species with 2n = 18 chromosome (subclade A´) separated from the other two species D. pusillus and D. muricatus, which were positioned into two individual clusters. The other clade includes the Daucus from the B group. It was also noticed that few accessions were intermixed amongst clusters. Different genetic diversity parameters were estimated based on the four clusters (populations) defined by STRUCTURE software, demonstrating that clusters 3 and 4 possessed the lowest and highest diversity values, respectively. AMOVA showed variation between and within clusters of 41.85% and 58.15%, respectively. The highest population divergence (Fst) was observed between clusters 2 and 3 (0.579), on the other hand, clusters 1 and 4 depicted the lowest Fst with 0.160. Our research highlighted that iPBS markers were successful and effective to study Daucus genetic diversity. These results will contribute to the genetic improvement of carrots and sustainable management of its diversity.
Microsatellite-based genetic diversity and population structure of Huacaya alpacas (Vicugna pacos) in Southern Peru
(MDPI, 2023-05-05) Figueroa Venegas, Deyanira Antonella; Corredor Arizapana, Flor Anita; Mamani Cato, Ruben; Gallegos Acero, Roberto; Condori Rojas, Nicoll; Estrada Cañari, Richard; Heredia Vilchez, Lizeth Amparo; Salazar Coronal, Wilian; Quilcate Pairazamán, Carlos Enrique; Arbizu Berrocal, Carlos Irvin
The alpaca population mostly consists of the Huacaya phenotype and is widely distributed in Southern Peru. This study aimed to estimate the genetic diversity and population structure of two Huacaya alpaca populations (Ajoyani and Quimsachata) using fourteen and twelve microsatellite markers for each population, respectively. A total of 168 alpaca biological samples were outsourced to Peruvian laboratories for DNA extraction and genotyping. For genetic diversity, observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and fixation indices values were estimated. An admixture analysis was performed for the population structure analysis. Different programs were used for these estimations. In total, 133 (Ajoyani) and 129 (Quimsachata) alleles were found, with a range of 4 to 17 by locus. The mean HO, HE, and PIC per marker for Ajoyani were 0.764 ± 0.112, 0.771 ± 0.1, and 0.736; for Quimsachata, they were 0.783 ± 0.087, 0.773 ± 0.095, and 0.738, respectively. The population structure showed no structure with K = 2. This study provides useful indicators for the creation of appropriate alpaca conservation programs.
The roles of breeding and husbandry for improving cavy production in Peru
(Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, 2017-08-10) Chauca Francia, Lilia Janine
Animal production is supported by four pillars: genetics, nutrition, livestock management and health. In Peru, the cavy program started making changes by handling the reproduction system. The population was organized for mating in groups with 1 male and 7 females; the feed was only forage, supplemented by a ration adding necessary nutrients. Cavies were selected for production characteristics that allowed the formation of breeds (‘razas’). Biosafety management programs were applied to prevent animals from getting sick. The genetic improvement program began with selecting two essential characteristics, resulting in economic benefits to the producer. Precocity permits cavies to come to market in less time (56 days old – breed ‘Peru’), with an efficient feed conversion ratio (2.68 : 1) and a carcass yield of 72%. Its relationship muscle : bone in the hind limb (hip, thigh and leg) is 5.6 : 1; and better muscle structure was determined by histological cuts. Prolificacy (breed ‘Andina’) determines a litter size of 3.9 offspring/delivery and the ability that 78% of the breeding present post-partum oestrus. Interracial crossbreeding allows a hybrid that improves productivity on farms by forming non-inbred populations. The optimum first breeding age was determined for females at 8 or 9 weeks and for males at 12 weeks. Oestrus synchronization allowed better management of nursing, with better environment and feed management. The mortality rate decreased achieving survival of larger litter size. In the highlands, improved productivity was attained by crossing improved cavies with the original native cavies, while preserving genetic diversity of the native animals. After years of research, producing cavies went from being just a domestic self-sustaining activity to a productive and large-scale activity that has generated jobs for rural women. Cavy meat consumption of children and pregnant women has helped to reduce malnutrition in Peru.