Examinando por Materia "Embryo transfer"
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Ítem Embryo transfer in camelids: Study of a reliable superovulatory treatment in llamas(Wageningen Academic Publishers, 2006-12-01) Huanca López, Wilfredo; Ratto, Marcelo; Santiani Acosta, Alexei Vicent; Cordero, Aida; Huanca Mamani, TeodosioSouth American Camelids are receiving increased interest not only in South America but also on a worldwide scale. They possess some unique features such as their fine fibre and their high adaptivity to many climatic regions across the world. Apart from the important productive aspects, their physical attractiveness also makes them popular as pet animals. However there are still many gaps in the scientific literature with regard to South American Camelids. This collection of papers brings experience of both South American and European experts together. It considers current trends in reproduction, nutrition, health, fibre morphology and genetics and discusses as new topic aspects of the potential of meat production and commercialization in South America. The particular advantages of South American Camelids for the sustainable use of fragile ecosystems with native pastures are outlined. Round tables discussions focus on the interaction between wild and domestic species, the management of alpaca populations outside of South America and health aspects under European conditions.Ítem Evaluación de la recuperación de embriones en alpacas y llamas donadoras simples y superestimuladas(Universidad Nacional Micaela Bastidas de Apurímac (UNAMBA), 2014-10-24) Huanca Mamani, Teodosio; Gonzáles Castillo, Mario Lino; Mamani Cato, Rubén Hebert; Cárdenas Minaya, Oscar Efraín; Sapana Valdivia, Rómulo; Naveros Flores, Mary LuzEl estudio se realizó en la estación experimental Quimsachata, del Instituto Nacional de Innovación Agraria (INIA), Puno, Perú a 4200 metros de altitud. El objetivo fue evaluar la recuperación de embriones en alpacas y llamas superestimuladas y donante simple. Para ello se han utilizado 181 alpacas divididas en dos grupos: donantes simples (ADS) 87 alpacas y donantes superestimuladas (ADSE) 94 alpacas; 158 llamas y divididas en dos grupos: donantes simples (LLDS) 87 llamas y donantes superestimuladas (LLDSE) 71 llamas. Los resultados muestran que el número de folículos preovulatorios y cuerpos lúteos superestimulados es mayor tanto en alpacas como en llamas (P < 0,01), el porcentaje y el número de embriones recuperados es mayor en alpacas y llamas donantes simples (P < 0. 01); el mayor porcentaje de embriones recuperados son de excelente calidad, seguido de bueno, regular, malo y esta misma proporción se mantiene en alpacas y llamas donantes simples y superestimuladas (P > 0.05); también los tamaños de los embriones recuperados de alpacas y llamas donantes simples y superestimuladas son similares (P > 0.05). Se concluye que es factible recuperar embriones transferibles de alpacas y llamas simples y superestimuladas donantes.Ítem Induction of superovulation in South American camelids(Elsevier B.V., 2013-01-10) Ratto, Marcelo H.; Silva, Mauricio E.; Huanca López, Wilfredo; Huanca Mamani, Teodosio; Adams, Gregg PatrickThe development of assisted reproductive technologies such as embryo transfer (ET), artificial insemination (AI) and in vitro fertilization (IVF) in South American camelids is considerably behind that of other livestock species. Poor success of the embryo transfer technique has been related to a lack of an effective superstimulatory treatment, low embryo recovery rate, and the recovery of hatched blastocysts that are not conducive to the cryopreservation process. Superstimulation has been attempted using equine chorionic gonadotropin (eCG) and follicle stimulating hormone (FSH) during the luteal, or the sexually receptive phase, sometimes given at follicular wave emergence. The rationale for inducing a luteal phase prior to or during superstimulation in camelids is not clearly understood, but it may simply reflect an empirical bias to conventional methods used in other ruminants. The number of ovulations or CL varies widely among studies, ranging from 2 to more than 15 per animal, with the number of transferable embryos ranging from 0 to 4 per animal. The control of follicular growth combined with superstimulatory protocols has resulted in a more consistent ovarian response and a greater number of follicles available for aspiration and oocyte collection. Recent studies in llamas have demonstrated that the use of ovulation inducing treatments or follicle ablation can synchronize follicular wave emergence allowing the initiation of gonadotropin treatment in the absence of a dominant follicle resulting in a more consistent ovulatory response. Few studies in alpacas have been reported, but it appears from recent field studies that the ovarian response is more variable and that there is a greater number of poor responders than in llamas. A review of superstimulation protocols that have been used in llamas and alpacas in the last 15 years is provided, including a discussion of the potential of protocols designed to initiate treatment at specific stages of follicular growth.