Examinando por Materia "Drought stress"

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Comparing the differential expression of selected genes in native Peruvian potatoes in response to early and late drought conditions
(Palm Beach State College, 2017-04-01) Cornelio, Laynet; Perez, Indira; Vazquez, Carlos; Martinez, Diana; Ponce, Olga Patricia; Murata, Emi; Torres, Yerisf; Zuñiga López, Luz Noemí; Orjeda, Gisella; Maul, Dora Pilar; Merino, Carlos
Plant response to drought stress comprises growth inhibition, stomatal closure, an increase in abscisic acid (ABA) biosynthesis and increased antioxidant metabolism, among others. Drought tolerance in plants is linked to photosynthesis and carbohydrate metabolism. Drought-inducible functional proteins include those that protect cells from water deficit and oxidation as well as transcription factors and signaling molecules associated with the water-stress response. Native potatoes from the Andean regions of Peru, Ecuador and Bolivia grow at altitudes as high as 11483 ft. (3500m) above sea level. Because of their high genetic diversity, they are well adapted to the harsh environmental conditions that prevail in the high Andes, including drought. This makes them ideal candidates for gene expression studies associated with drought tolerance. St. Thomas University is collaborating with the Universidad Peruana Cayetano Heredia (UPCH, Lima, Peru), and the Instituto Nacional de Innovacion Agraria (INIA, Huancayo, Peru) in a gene expression study in native potatoes associated with early and late drought responses. Selected drought associated candidate genes from RNA-seq analysis were used in primer design and quantitative RT-PCR analysis. Differential gene expression in tolerant vs. susceptible cultivars has been confirmed for two heat shock proteins, a dehydration-response element binding protein and for a major pollen allergen.
Development of an open-source thermal image processing software for improving irrigation management in potato crops (Solanum tuberosum L.)
(MDPI, 2020-01-14) Cucho Padin, Gonzalo; Rinza, Javier; Ninanya, Johan; Loayza, Hildo; Quiroz, Roberto; Ramirez, David A.
Accurate determination of plant water status is mandatory to optimize irrigation scheduling and thus maximize yield. Infrared thermography (IRT) can be used as a proxy for detecting stomatal closure as a measure of plant water stress. In this study, an open-source software (Thermal Image Processor (TIPCIP)) that includes image processing techniques such as thermal-visible image segmentation and morphological operations was developed to estimate the crop water stress index (CWSI) in potato crops. Results were compared to the CWSI derived from thermocouples where a high correlation was found (𝑟𝑃𝑒𝑎𝑟𝑠𝑜𝑛 = 0.84). To evaluate the effectiveness of the software, two experiments were implemented. TIPCIP-based canopy temperature was used to estimate CWSI throughout the growing season, in a humid environment. Two treatments with different irrigation timings were established based on CWSI thresholds: 0.4 (T2) and 0.7 (T3), and compared against a control (T1, irrigated when soil moisture achieved 70% of field capacity). As a result, T2 showed no significant reduction in fresh tuber yield (34.5 ± 3.72 and 44.3 ± 2.66 t ha−1), allowing a total water saving of 341.6 ± 63.65 and 515.7 ± 37.73 m3 ha−1 in the first and second experiment, respectively. The findings have encouraged the initiation of experiments to automate the use of the CWSI for precision irrigation using either UAVs in large settings or by adapting TIPCIP to process data from smartphone-based IRT sensors for applications in smallholder settings.
Estimación de la evapotranspiración a partir de imágenes de alta resolución de VANT para sistemas de riego en arrozales de la costa norte de Perú
(Universidad Nacional de Trujillo. Facultad de Ciencia Agropecuarias, 2024-02-05) Ramos Fernández, Lia; Quispe Tito, David; Altamirano Gutiérrez, Lisette; Cruz Grimaldo, Camila Leandra; Quille Mamani, Javier Alvaro; Carbonell Rivera, Juan Pedro; Torralba, Jesús; Ángel Ruiz, Luis
Ante la creciente escasez del agua para la agricultura, el incremento de la demanda de alimentos y los futuros escenarios de sequía que nos plantea el cambio climático es indispensable diseñar nuevas tecnologías que contribuyan a un menor consumo de agua. En esta investigación se han empleado imágenes de alta resolución para estimar la evapotranspiración en arrozales aplicando el modelo de balance de energía METRICTM (Mapping Evapotranspiration at High Resolution using Internalized Calibration). Para ello, se monitorizaron 5900 m2 de cultivo bajo riego por inundación continua (IC) y 2600 m2 bajo la técnica de riego de alternancia humedecimiento y secado (AWD, por sus siglas en inglés), además de algunas parcelas con filtración lateral. Se realizaron 10 vuelos entre las etapas de macollamiento y floración, cinco vuelos con un VANT Matrice 210 con una cámara multiespectral Parrot Sequoia, y cinco vuelos con un Matrice 300 RTK equipado con una cámara térmica H20T. Se colectó información de campo de los índices de vegetación (NDVI e IAF), y lecturas de un radiómetro, para ajustar información de las imágenes multiespectrales y térmicas, respectivamente; y obtener los componentes del balance de energía en superficie. Se obtuvo valores medios para evapotranspiración del cultivo (ETc) de 6,34 ±1,49 y 5,84 ± 0,41 mm d-1 para riego IC y riego AWD, respectivamente, obteniéndose un ahorro de agua del 42% con una reducción del rendimiento en 14%, proporcionando una guía para la gestión adecuada del riego, sin embargo, se sugiere utilizar el modelo para optimizar el rendimiento obteniendo umbrales críticos para la aplicación óptima de AWD frente a la escasez del recurso hídrico.
Unraveling ecophysiological mechanisms in potatoes under different irrigation methods: a preliminary field evaluation
(MDPI, 2020-06-11) Silva Díaz, Cecilia; Ramírez, David A.; Rodríguez Delfín, Alfredo; De Mendiburu, Felipe; Rinza, Javier; Ninanya, Johan; Loayza, Hildo; Quiroz, Roberto
Potatoes—a global food security and staple crop—is threatened by dry spells in drought-prone areas. The use of physiological thresholds to save water while maintaining a reasonable tuber yield has been proposed, but their effects on physiological performances and usefulness under different irrigation methods are yet to be evaluated. In this study, photosynthetic traits were monitored to assess the effect of water restriction and rewatering under drip (DI) and furrow (FI) irrigations. The treatments consisted of two maximum light-saturated stomatal conductance (g𝑠_𝑚𝑎𝑥) irrigation thresholds (T2: 0.15 and T3: 0.05 mol H2O m−2 s−1) compared with a fully irrigated control (g𝑠_𝑚𝑎𝑥 > 0.3 mol H2O m−2 s−1). DI used less water than FI but promoted early senescence and low percentage of maximum assimilation rate (PMA) at late developmental stages. FI caused no yield penalization in T2 and higher recovery of carbon isotope discrimination and PMA than DI. It is suggested that moderate water quantities of early and frequently water pulses in the irrigation, promote short-term water stress memory improvement, senescence delay and more capability of recovery at late stages.
Water saving using thermal imagery-based thresholds for timing irrigation in potatoes under drip and furrow irrigation systems
(MDPI, 2022-11-23) Rinza, Javier; Ramírez, David A.; Ninanya, Johan; De Mendiburu, Felipe; García, Jerónimo; Quiroz, Roberto
Under the current water crisis in agriculture, irrigation methods for saving and conserving water are necessary. However, these methods must guarantee an appropriate yield with a concomitant economic benefit and a reduced environmental impact. In this study, two irrigation thresholds for irrigation timing (IT) based on thermal imagery were analyzed with the UNICA potato variety in three trials under drip (DI) and furrow (FI) irrigation during 2017–2018 in Lima, Peru. The control (T1) remained at >70% of soil field capacity. For other treatments, thresholds were defined based on stomatal conductance at light saturation (T2: 0.15 and T3: 0.05 mol H2O m−2 s−1) and crop water stress index (T2: 0.4 and T3: 0.6) based on canopy temperature. An integrated index (IIN) was established for the valuation of treatments using the criteria of high fresh tuber yield (FTY) and a low total amount of irrigated water, production cost (PC), and total C emissions (TE) and using criteria of a score. FI-T2 (0.69–0.72) and DI-T3 (0.19–0.29) showed the highest and lowest IIN value, respectively. FTY in T2 was not significantly reduced under FI, resulting in a lower PC regarding DI–T2 and emphasizing the usefulness of thermal imagery in determining watering schedules in potatoes under furrow irrigation systems.