Examinando por Materia "Cloroplasto"

Mostrando 1 - 2 de 2

Análisis del genoma del cloroplasto del maíz morado INIA 601 para reconstruir la historia evolutiva del maíz morado peruano
(Universidad Nacional Agraria La Molina, 2019-10) Rodríguez Pérez, Lila M.; Montenegro, Juan D.; Simon, Reinhard; Chumbe Nolasco, Lenin; Serna Chumbes, Manuel Fernando; Delgado, Gabriel; García Serquén, Aura Liz; Gutiérrez Reynoso, Dina Lida
En el presente estudio, se ha reconstruido la secuencia completa del genoma plastidial del maíz morado peruano y se ha comparado con otros genomas plastidiales de maíces. El genoma plastidial tiene una longitud de 140,458 pb y muestra una estructura típica del genoma del cloroplasto: un par de regiones repetidas invertidas (IRa e IRb) de 22,594 pb, una región Larga de Copia Única (LSC) de 82,472 pb, y una región Corta de Copia Única (SSC) de 12,798 pb. Las relaciones filogenéticas fueron obtenidas a partir de alineamientos genómicos completos con los genomas plastidiales de otros miembros del género Zea. Los resultados indicaron que el maíz morado peruano está más relacionado a Z. mays subsp. huehuetenangensis. Es posible que eventos de hibridación introgresiva a lo largo de la evolución del maíz morado hayan jugado un papel en la adquisición del fenotipo morado en el clado Z. mays.
Revealing the complete chloroplast genome of an Andean horticultural crop, sweet cucumber (Solanum muricatum), and its comparison with other Solanaceae species
(MDPI, 2022-09-01) Saldaña Serrano, Carla Lizet; Chávez Galarza, Julio César; De la Cruz, Germán; Jhoncon, Jorge H.; Guerrero Abad, Juan Carlos; Vásquez Pérez, Héctor Vladimir; Maicelo Quintana, Jorge Luis; Arbizu Berrocal, Carlos Irvin
Sweet cucumber (Solanum muricatum) sect. Basarthrum is a neglected horticultural crop native to the Andean region. It is naturally distributed very close to other two Solanum crops of high importance, potatoes, and tomatoes. To date, molecular tools for this crop remain undetermined. In this study, the complete sweet cucumber chloroplast (cp) genome was obtained and compared with seven Solanaceae species. The cp genome of S. muricatum was 155,681 bp in length and included a large single copy (LSC) region of 86,182 bp and a small single-copy (SSC) region of 18,360 bp, separated by a pair of inverted repeats (IR) regions of 25,568 bp. The cp genome possessed 87 protein-coding genes (CDS), 37 transfer RNA (tRNA) genes, eight ribosomal RNA (rRNA) genes, and one pseudogene. Furthermore, 48 perfect microsatellites were identified. These repeats were mainly located in the noncoding regions. Whole cp genome comparative analysis revealed that the SSC and LSC regions showed more divergence than IR regions. Similar to previous studies, our phylogenetic analysis showed that S. muricatum is a sister species to members of sections Petota + Lycopersicum + Etuberosum. We expect that this first sweet cucumber chloroplast genome will provide potential molecular markers and genomic resources to shed light on the genetic diversity and population studies of S. muricatum, which will allow us to identify varieties and ecotypes. Finally, the features and the structural differentiation will provide us with information about the genes of interest, generating tools for the most precise selection of the best individuals of sweet cucumber, in less time and with fewer resources.