Examinando por Materia "Antioxidantes"

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Evaluación de la actividad antioxidante de la pulpa, cáscara y semilla del fruto del camu camu (Myrciaria dubia H.B.K.)
(Sociedad Química del Perú, 2009-07-31) Sotero Solís, Víctor; Silva Doza, Luz; García de Sotero, Dora; Imán Correa, Sixto Alfredo
[ES] El camu camu es una especie nativa de la Amazonía y el interés por su fruto radica en su alta concentración de ácido ascórbico. En el presente estudio se realizó la determinación de la actividad antioxidante de la pulpa, cáscara y semilla del camu camu colectado en el banco de Germoplasma del INIA-Loreto. Se realizó la evaluación de la actividad antioxidante, mediante el secuestro de radicales libres del DPPH. Se determinó la concentración de compuestos fenólicos y ácido ascórbico, mediante el método espectrofotométrico y por cromatografía de HPLC. Se observa que los mejores resultados en cuanto a la actividad antioxidante la presentó la cáscara de camu camu con IC50 de 146,94 μg/ml, seguido de la pulpa con 167,67 μg/ml y con menor actividad la semilla con 399,77 μg/ml. Las mejores concentraciones para compuestos fenólicos se obtienen en pulpa seca (23168,0 mg/100g) y en cáscara seca (17905,5 mg/100g). Las concentraciones de ácido ascórbico en peso seco, reportan la mayor concentración en pulpa: 14337,94 mg/100g y cáscara: 10506,37 mg/100g, siendo inferior en semilla: 87,08 mg/100g,. En la pulpa y cáscara del camu camu se encontraron ácido clorogénico, catequina, epicatequina y rutina, destacando la catequina en cáscara con 47,29 mg/100g. --------------- [EN] Camu camu is a native specie of theAmazonia and the interest for its fruit takes root in its high concentration of ascorbic acid, beside possessing excellent antioxidant qualities. In the present study there was realized the evaluation of the antioxidant activity of the pulp, peel and seed of the camu camu collected in Germoplasma's bank of the INIA-Loreto. There was realized the evaluation of the antioxidant activity, by means of the scavenging of free radical of the DPPH. Likewise they were realized the analysis of phenolics compounds and ascorbic acid, by means of the spectrophotometric method and HPLC chromatography. It is observed that the best results for the antioxidant activity was the peel of camu camu with IC50 of 146,94 μg/ml, followed by the pulp with 167,67 μg/ml and with minor activity the seed with 399,77 μg/ml. The best concentrations for the compounds phenolics are obtained in dry pulp (23168,0 mg/100g) and in dry peel (17905,5 mg/100g). The concentrations of ascorbic acid reports the major concentration in dry pulp: 14337,94 mg/100g and peel: 10506,37 mg/100g, being low in seed: 87,08 mg/100g. In the pulp and peel of camu camu they were chlorogeneic acid, catechin, epicatechin and rutin, emphasizing the catechin in rind with 47, 29 mg/100g.
Study of the physicochemical characteristics, antimicrobial activity, and in vitro multiplication of wild blackberry species from the Peruvian highlands
(Nature Publishing Group, 2024-02-16) Lapiz Culqui, Yoiner K.; Meléndez Mori, Jegnes Benjamín; Tejada Alvarado, José Jesús; Cortez, Denny
The Peruvian Andes are the natural habitat of several wild blackberry species that are little known and exploited due to the lack of technological and scientific development to support their agricultural potential. In this context, a study was conducted to understand the physicochemical composition, bioactive compounds, antimicrobial activity, and in vitro multiplication of four wild blackberry (Rubus sp.) species from the northern Peruvian highlands. The results indicate that fruits of R. floribundus presented the highest content of total soluble solids (9.58 ± 1.83°Brix) and titratable acidity (1.88 ± 0.07% citric acid). The fruits of R. weberbaueri recorded the highest total phenolic content (415.06 ± 8.69 mg GAE/100 g Ff). The antioxidant capacity determined by the DPPH assay varied significantly among species, with the highest value found in fruits of R. andicola (50.27 ± 0.11 mg TE/100 g Ff). The fruit extracts of R. weberbaueri and R. andicola showed better antimicrobial activity, with Staphylococcus aureus being the most sensitive bacterium. In the in vitro multiplication phase, the results show that BAP (6-Benzylaminopurine) has a significant effect at a dose of 1.5 mg l−1 on shoot number, leaf number, and shoot length. The results may help in the management of genetic resources.