Examinando por Autor "Rivera Jacinto, Marco"

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Caracterización de la crianza de cuyes en tres provincias de la Región Cajamarca, Perú
(Universidad Nacional Mayor de San Marcos, 2021-04-23) Ortiz Oblitas, Pedro; Florián Alcántara, Amarante Nicolas; Estela Manrique, Judith Raquel; Rivera Jacinto, Marco; Hobán Vergara, Cristian; Murga Moreno, César
El presente estudio tuvo como objetivo la caracterización de la crianza de cuyes en tres provincias de la Región Cajamarca, Perú. Se aplicó una encuesta estructurada a 1231 productores (401 de Cajamarca, 360 de San Marcos y 470 de Cajabamba). El sistema de producción predominante es el familiar-comercial (67% de productores). La raza Perú es la preferida por los productores, utilizando mayormente el sistema de crianza en pozas. La mayoría de los productores posee menos de 49 cuyes con un área de terreno entre 0.5 a 5 ha., siendo la alfalfa (Medicago sativa) el forraje más utilizado en la alimentación de los cuyes. Más del 80% de productores no reciben asistencia técnica y más del 87% no forman parte de alguna asociación de criadores. En la caracterización sanitaria, entre 1 y 5 cuyes mueren por semana y por productor, especialmente en la clase lactantes. La enfermedad más reportada es la salmonelosis, la cual es identificada por las alteraciones en hígado.
Molecular diagnosis of Sarcocystis aucheniae by semi-nested polymerase chain reaction in blood from alpacas (Vicugna pacos) in Peru
(Faculty of Veterinary Medicine, University of Tripoli, 2026-07-20) Rojas Valdez, Deisy Janeth; Rivera Jacinto, Marco; Cueva Rodríguez, Medali; Tayca Saldaña, Antony; Bujaico Mauricio, Norma; Cardenas Minaya, Oscar Efraín; Alvarez García, Wuesley Yusmein; Cabrera González, Marco Antonio
Background: Sarcocystis aucheniae is the causative agent of sarcocystosis in South American camelids, such as alpacas. The disease is generally asymptomatic in its acute phase and is characterized by the presence of macroscopic cysts in skeletal muscles, which limits the commercialization of alpaca meat. Aim: This study aimed to establish the analytical sensitivity and specificity of the semi-nested polymerase chain reaction (PCR) molecular technique for the diagnosis of S. aucheniae in the blood of alpacas from the province of Huancavelica, Peru. Methods: The analytical sensitivity of semi-nested PCR was determined using very low concentrations of genomic DNA extracted from S. aucheniae macrocysts. The semi-nested PCR assay was compared with the results of the molecular test on blood and the necropsy method. For this purpose, genomic DNA was isolated from blood samples from Huancavelica (n = 8) and Cajamarca (n = 7) alpacas. Furthermore, a previous necropsy was conducted on the muscle tissue of these animals. Furthermore, 42 additional blood samples were collected from alpacas in Huancavelica to detect the 18S rRNA gene of S. aucheniae by semi-nested PCR. Macrocyst DNA was used as a positive control, whereas blood from a negative alpaca for the parasite was used as a negative control. Results: The analytical sensitivity of semi-nested PCR had a detection limit of one picogram of DNA, and the specificity of the technique was 80%. Thirty-two percent (n = 16) of Huancavelica alpacas tested positive for the parasite by semi-nested PCR. Conclusions: Semi-nested PCR had both a satisfactory detection limit and a high degree of specificity for the diagnosis of S. aucheniae in alpaca blood