Examinando por Autor "Gutierrez Reynoso, Dina Lida"

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Molecular characterization and first report of entomopathogenic nematodes from carob forests in Tumbes, Peru
(Agrociencia Uruguay, 2026-08-07) Riojas Gonzales, J.M.; Castillo Carrillo, P.S.; Cornejo Hidalgo, R.E.; Mogollón Farias, Cesar Augusto; Garcia Garcia, Segundo Melecio; Temoche Socola, V.T.; Ruiz Polo, Archi Alejandro; Gutierrez Reynoso, Dina Lida; Fernandez Huaytalla, Elizabeth; Cordova Campos, José Stalyn
Given their ability to attack and eliminate insects that impact various ecosystems, entomopathogenic nematodes (EPNs) are considered effective biological pest control agents. However, there are few studies focused on their identification or their association with forest species such as the carob tree. The objective of the present study was to isolate and molecularly identify entomopathogenic nematodes from carob tree (Neltuma spp.) forests in Tumbes, Peru. One-kilogram soil samples were collected from ten localities located across four districts in the Tumbes region. In each soil sample, Galleria mellonella Linnaeus 1758 (Lepidoptera: Pyralidae) larvae were exposed as bait for the detection and isolation of EPNs. Exposure times were controlled at 12, 24, 48, 72, and 84-96 hours. The isolated nematodes were morphologically characterized, and their genomic DNA was extracted. The 18S ribosomal DNA (rDNA) gene was amplified via polymerase chain reaction (PCR). Six of the ten evaluated soils showed G. mellonella infection, with a positive correlation between exposure time and infection rate. However, due to marked infectivity divergences (where samples 02 and 06 exhibited the highest percentages), three representative strains were selectively isolated from soil samples 02, 05, and 06. Subsequent morphological characterization and molecular identification determined their taxonomic identity as Heterorhabditis bacteriophora and Heterorhabditis indica. This finding represents the first report of EPNs associated with carob tree plantations in northern Peru and highlights their pathogenic potential against insect larvae. It is recommended that the nematodes identified at the species level be considered in integrated pest management (IPM).
The complete chloroplast genome of a centennial olive tree (Olea europaea, Oleaceae) from the southern Peruvian coast
(F1000Research, 2024-12-04) Saldaña, Carla L.; Chura Llanos, Luz S.; Estrada Cañari, Richard; Fernandez Huaytalla, Elizabeth; Zirena Vilca, Franz; Guerrero Abad, Juan Carlos; Amasifuen Guerra, Carlos Alberto; Gutierrez Reynoso, Dina Lida; Injante Silva, Pedro Hugo; Arbizu, Carlos I
Olive (Olea europaea Linaeus 1753) is one of the valuable fruit trees and very important edible oil plant in the world. The chloroplast (cp) genome of an olive tree (Olea europaea) from the southern Peruvian arid coast was obtained for the first time. Genomic DNA of high quality was used to generate librarieswith Illumina Hiseq paired-end methods. The cp genome is 155,886 pb in length and contains a large single-copy region (LSC) of 86,610 pb and a small single copy region (SSC) of 17,790 pb separated by two inverted repeat (IR) regions (25,741bp). The cp genome of olive contains 124 genes that consists of 80 protein-coding genes, 36 tRNA, eight rRNA. Phylogenetic analysis showed this olive tree is sister to O. europaea subsp. maroccana ( Oleeae tribe). This study presents the first overview of the chloroplast genome organization and phylogenetics of O. europaea, offering valuable insights for genetic and evolutionary research in the genus Olea.