Examinando por Autor "Guillen Palomino, Crissthel Yverlin"

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Electrophoretic profiles of alpaca seminal plasma proteins and their association with sperm quality parameters during cryopreservation processes
(John Wiley & Sons Ltd., 2026-03-24) Guillen Palomino, Crissthel Yverlin; Mujica Lengua, Fidel Rodolfo; Contreras Huamaní, Mijail; Carretero , María Ignacia; Rueda Alfonso, Fabian Leonardo; Orellana Berrocal, Harumi
The aim of the study was to characterize the electrophoretic profiles of alpaca seminal plasma (SP) proteins and establish their association with sperm quality parameters at different cryopreservation stages. Sperm quality was assessed in raw, cooled, and thawed semen from 128 ejaculates collected from 16 Huacaya alpacas, and SP proteins were analysed by SDS-PAGE in raw samples. Statistical associations were determined using Spearman's rank correlation (p ≤ 0.05). Twenty-three protein bands were identified: 21 bands ranging from 9.23 to 138.38 kDa, and 2 below 6.5 kDa. Notably, the 21.03 kDa protein was absent in six males, five of whom also lacked the 18.88 kDa band. These individuals exhibited superior post-thaw sperm quality, particularly higher motility. The 21.03 kDa protein showed a negative correlation (p ≤ 0.05) with sperm motility and membrane function in raw, cooled, and thawed semen, and a positive correlation with acrosome integrity in thawed semen. Similarly, the 18.88 kDa protein showed a negative correlation with sperm motility and membrane function, but a positive correlation with acrosome integrity in thawed semen (p ≤ 0.05). In conclusion, these findings suggest that specific SP proteins may serve as potential biomarkers for sperm quality and cryotolerance in alpacas, reflecting individual variability in response to cryopreservation.
Influence of reproductive season and testicular volume on seminal parameters of alpacas (Vicugna pacos)
(Springer Nature, 2025-05-22) Contreras Huamani, Mijail; Guillen Palomino, Crissthel Yverlin; Orellana Berrocal, Harumi Rebeca; Laines Arcce, Irwin Mitchell; Bellido Quispe, Dionet Keny; Acuña Leiva, Alex Yony; Silva Chaves, Maiana; de Figueirêdo Freitas, Vicente José
The male alpaca plays an important role in the reproductive process and, consequently, in genetic improvement. South American camelids (SACs) have a well-defined reproductive season, particularly in the Andean region. The objective was to evaluate the influence of reproductive and non-reproductive seasons, as well as testicular volume, on seminal parameters in alpacas. Nine healthy male alpacas, over 4 years old, were selected for this experiment. Semen samples were taken every two days using an artificial vagina incorporated into a dummy. The collected were in the reproductive (November to April) and non-reproductive (May to October) seasons, from breeders with testicular volumes that range between 63–70 cm3 and 83–94 cm3. The total number of ejaculates collected was 72 per season, with 8 samples collected from each male. Macroscopic aspects such as color, volume (ml), thread formation (cm) and foam (cm) were evaluated, in addition to microscopic aspects such as sperm motility (%), viability (%) concentration (sperm/ml) and total number of sperm. The results were analyzed with the Mann–Whitney non-parametric test, using the R 3.4.3 software, considering P < 0.05 as the significance level. A decrease in thread formation was observed in semen collected during the reproductive season compared to the non-reproductive season (P < 0.05). On the other hand, sperm motility and viability in semen collected during the reproductive period was higher (p < 0.05). Regarding testicular volume, alpacas with larger testicular volume had greater ejaculate volume, sperm concentration and total number of sperm (p < 0.05). Based on these results, it is possible to conclude that the semen of alpaca breeders is influenced by the season, and that testicular volume can predict their reproductive capacity, being a parameter to be considered when selecting the best breeding male.