Examinando por Autor "Guelac Santillan, Marly"

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Effect of arbuscular mycorrhizae on the growth of Cinchona officinalis L. (Rubiaceae) in nursery
(Taylor & Francis Group, 2024-06-11) Fernandez Zarate, Franklin Hitler; Huaccha Castillo, Annick Estefany; Quiñones Huatangari, Lenin; Vaca Marquina, Segundo Primitivo; Sanchez Santillan, Tito; Guelac Santillan, Marly; Seminario Cunya, Alejandro
Cinchona officinalis, commonly called cascarilla or quina, has medicinal value; and is on Peru’s national coat of arms representing its plant wealth (flora), however, it is threatened by anthropogenic activities. This study aimed to determine the effect of the commercial product Myco Grow® on the growth of C. officinalis in nursery. A randomized design was used with two treatments, one with Myco Grow® application (WM) and the other without incorporating this commercial product (NM). Each treatment had three replicates consisting of 30 plants each. Monthly evaluations were performed, during which the number of dead plants, plant height, and plant diameter were recorded. Additionally, at the end of the study, the anhydrous weight of leaves, stems, and roots; leaf area; mycorrhizal frequency; mycorrhizal colonization index; and the length of extra-radicular mycelia were determined. The WM treatment achieved 36.6% lower mortality, 38.01% greater height, and 48.52% greater diameter than the NM treatment. Additionally, inoculation with arbuscular mycorrhizae (AM) improved the anhydrous weights of the leaves, stems, roots, and leaf area by 84.31%, 84.28%, 70.85%, and 76.91%, respectively. Regarding the three fungal variables analyzed for the WM treatment; mycorrhizal frequency was 87%, AM application led to a mycorrhizal intensity of 7.7% and an extra-radicular mycelium length of 90.3 cm. This study confirmed that AM positively influences the growth of C. officinalis in the nursery and can be used to sustainably produce this species on a large scale.
Metabarcoding reveals rhizosphere microbiome differences in healthy and basal rot-affected dragon fruit plants
(Elsevier B.V., 2026-02-18) Guelac Santillan, Marly; Fernandez Castro, Paul; Huaman Pilco, Angel F.; Estrada Cañari, Richard; Rodríguez Grados, Pedro; Arbizu, Carlos I.
The rhizosphere microbiome plays a crucial role in plant health, yet its dynamics in Selenicereus megalanthus (yellow dragon fruit) remain poorly understood. This study employed high-throughput sequencing to characterize the bacterial and fungal communities in the rhizosphere of healthy and basal rot-affected plants across four commercial production sites in Amazonas department from Peru. Amplicon sequencing Metagenomics Sequencing (WOBI) targeting to 16S rRNA (for bacteria) and ITS (for fungi) gene regions show differences in microbial community structure associated with plant health status. Multivariate analyses revealed a clear disease-driven reassembly of the bacterial microbiome, marked by the loss of health-associated taxa (Xanthobacteraceae, Geminicoccaceae, Nocardioidaceae) and enrichment of oligotrophic and stress-tolerant groups (Nitrososphaeraceae, Acidobacteriaceae Subgroup 1). In contrast, fungal assemblages displayed structural inertia, responding primarily through pathogen-associated increases in Nectriaceae. Soil physicochemistry particularly pH, exchangeable aluminum, and nutrient levels modulated the strength of bacterial differentiation, highlighting the role of edaphic filters in microbiome resilience. Our findings provide evidence of a bacterial-centered dysbiosis associated with basal stem rot in S. megalanthus, while positioning fungal communities as structurally resilient components of the holobiont. Together, these results outline a framework in which disease is linked to altered plant microbe soil feedbacks rather than pathogen presence alone, and suggest that bacterial assemblages could inform the development of microbiome-based early-warning indicators and soil health strategies for sustainable dragon fruit management.
Physiological response of Cinchona officinalis L., the Peruvian national tree, inoculated with native arbuscular mycorrhizal fungi under nursery conditions in the Amazon rainforest
(Universidad Nacional de Tumbes, 2026-06-30) Guelac Santillan, Marly; Huaman Pilco, Angel F.; Zabaleta Santisteban, Jhon A.; Vargas , Raúl; Corazon Guivin, Mike Anderson; Marcelo Bazán, Fátima Elizabeth; Sanchez Santillan, Tito
This study evaluated the agronomic response of Cinchona officinalis L. to inoculation with native arbuscular mycorrhizal fungi (AMF) under nursery conditions in the province of Condorcanqui, Amazonas, at 315 m.a.s.l., an unusually low elevation for a species typically found between 1000 and 3000 m.a.s.l. The main objective was to assess the physiological response of C. officinalis to AMF inoculation in lowland conditions. A completely randomized design was implemented, including five native AMF consortia and one non-inoculated control. Growth and symbiotic parameters such as plant height, dry biomass, mycorrhizal colonization, and spore diversity were measured. Results showed that inoculation with AMF consortia from San Jerónimo and Yambrasbamba significantly enhanced plant growth and symbiosis, indicating a positive effect on early development. These findings suggest that AMF play a key role in facilitating early establishment and seedling performance under low-elevation nursery conditions. This is one of the first reports demonstrating the potential of using native AMF to promote the successful propagation of this important forest species outside its natural altitudinal range, offering a sustainable strategy for conservation and restoration efforts in the Peruvian Amazon.