Examinando por Autor "Contreras Huamaní, Mijail"

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Electrophoretic profiles of alpaca seminal plasma proteins and their association with sperm quality parameters during cryopreservation processes
(John Wiley & Sons Ltd., 2026-03-24) Guillen Palomino, Crissthel Yverlin; Mujica Lengua, Fidel Rodolfo; Contreras Huamaní, Mijail; Carretero , María Ignacia; Rueda Alfonso, Fabian Leonardo; Orellana Berrocal, Harumi
The aim of the study was to characterize the electrophoretic profiles of alpaca seminal plasma (SP) proteins and establish their association with sperm quality parameters at different cryopreservation stages. Sperm quality was assessed in raw, cooled, and thawed semen from 128 ejaculates collected from 16 Huacaya alpacas, and SP proteins were analysed by SDS-PAGE in raw samples. Statistical associations were determined using Spearman's rank correlation (p ≤ 0.05). Twenty-three protein bands were identified: 21 bands ranging from 9.23 to 138.38 kDa, and 2 below 6.5 kDa. Notably, the 21.03 kDa protein was absent in six males, five of whom also lacked the 18.88 kDa band. These individuals exhibited superior post-thaw sperm quality, particularly higher motility. The 21.03 kDa protein showed a negative correlation (p ≤ 0.05) with sperm motility and membrane function in raw, cooled, and thawed semen, and a positive correlation with acrosome integrity in thawed semen. Similarly, the 18.88 kDa protein showed a negative correlation with sperm motility and membrane function, but a positive correlation with acrosome integrity in thawed semen (p ≤ 0.05). In conclusion, these findings suggest that specific SP proteins may serve as potential biomarkers for sperm quality and cryotolerance in alpacas, reflecting individual variability in response to cryopreservation.
Evaluation of probiotic supplementation to enhance growth, immune response, intestinal function, and survival in extensively raised neonatal alpacas in the Peruvian Central-southern Andes
(Elsevier Ltd., 2026-04-15) Palomino Guerrera, Walter; Mariño Huaraca, Wenfil Daisi; Contreras Huamaní, Mijail; Coila, Pedro; Taboada, Natalia Verónica
This study aimed to evaluate the effects of multi-strain probiotics on growth, immune response, intestinal function, and survival in neonatal alpacas in the Peruvian Central-southern Andes. A total of 96 neonatal alpacas were selected, with an average weight of 7.89 ± 0.93 kg, regardless of breed (Huacaya and Suri). The study employed a completely randomized design. The neonates were randomly assigned to three groups (n = 32): control (T1), Probiolyte® WS (T2), and Polimeve Solúve (T3). Probiotics were administered orally on days 1, 7, 14, 21, and 30 of age. Body weight gain and size were recorded on days 1, 15, 30, 60, and 90; immune response was assessed on 30 and 90 days; and intestinal functional status was evaluated on day 90. The neonates supplemented with Polimeve exhibited greater thoracic circumference after 60 days of age, and increased height at the withers and rump at 15 days (p < 0.05). At 90 days, the Polimeve group had a higher proportion of basophils, while the Probiolyte and control groups showed higher proportions of monocytes (p < 0.05). IgG concentrations at 30 and 90 days did not differ significantly among groups. Probiotic supplementation did not affect ileal villus length; however, it influenced the depth of jejunal and cecal crypts (p < 0.05). Morbidity and mortality rates did not differ significantly between groups. In conclusion, probiotics did not significantly influence growth, immune response, or intestinal function; however, Polimeve may improve weight gain in neonates, while Probiolyte can increase IgG concentrations during the first 30 days of life.