Examinando por Autor "Cardenas Minaya, Oscar Efraín"

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Efecto de la hora de siembra, edad y número de cuerpos luteos en receptoras de embriones criopreservados de ovino de la raza Dohne Merino
(Asociación Peruana de Reproducción Animal, 2012-08-31) Gonzáles Castillo, Mario Lino; Cardenas Minaya, Oscar Efraín; Sapana Valdivia, Romulo
La transferencia de embriones en ovinos permite introducir nuevas razas mediante la implementación de núcleos genéticos élite para reconformar la ganadería, mejorar la rentabilidad y su competitividad. Willadsen et al. (1976) lograron los primeros corderos a partir de embriones congelados. La transferencia de embriones descongelados implica la adaptación de la cría al medio ambiente de la receptora, en parte, debido al aporte de anticuerpos maternos durante la gestación y lactancia (Mc Ginnis et al., 1993); independientemente de la técnica de sincronización utilizada, esta debe ser eficiente y confiable (Rangel, 1997) para lograr una tasa mayor de preñez en receptoras en condiciones de sierra. El objetivo del estudio fue determinar el efecto de la hora de siembra embrionaria, la edad y el número de cuerpos lúteos en la fertilidad de la borrega receptora de embriones criopreservados de la raza Donhe Merino.
Molecular diagnosis of Sarcocystis aucheniae by semi-nested polymerase chain reaction in blood from alpacas (Vicugna pacos) in Peru
(Faculty of Veterinary Medicine, University of Tripoli, 2026-07-20) Rojas Valdez, Deisy Janeth; Rivera Jacinto, Marco; Cueva Rodríguez, Medali; Tayca Saldaña, Antony; Bujaico Mauricio, Norma; Cardenas Minaya, Oscar Efraín; Alvarez García, Wuesley Yusmein; Cabrera González, Marco Antonio
Background: Sarcocystis aucheniae is the causative agent of sarcocystosis in South American camelids, such as alpacas. The disease is generally asymptomatic in its acute phase and is characterized by the presence of macroscopic cysts in skeletal muscles, which limits the commercialization of alpaca meat. Aim: This study aimed to establish the analytical sensitivity and specificity of the semi-nested polymerase chain reaction (PCR) molecular technique for the diagnosis of S. aucheniae in the blood of alpacas from the province of Huancavelica, Peru. Methods: The analytical sensitivity of semi-nested PCR was determined using very low concentrations of genomic DNA extracted from S. aucheniae macrocysts. The semi-nested PCR assay was compared with the results of the molecular test on blood and the necropsy method. For this purpose, genomic DNA was isolated from blood samples from Huancavelica (n = 8) and Cajamarca (n = 7) alpacas. Furthermore, a previous necropsy was conducted on the muscle tissue of these animals. Furthermore, 42 additional blood samples were collected from alpacas in Huancavelica to detect the 18S rRNA gene of S. aucheniae by semi-nested PCR. Macrocyst DNA was used as a positive control, whereas blood from a negative alpaca for the parasite was used as a negative control. Results: The analytical sensitivity of semi-nested PCR had a detection limit of one picogram of DNA, and the specificity of the technique was 80%. Thirty-two percent (n = 16) of Huancavelica alpacas tested positive for the parasite by semi-nested PCR. Conclusions: Semi-nested PCR had both a satisfactory detection limit and a high degree of specificity for the diagnosis of S. aucheniae in alpaca blood