Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12955/1480
Título : Molecular tools against the illegal exploitation of the threatened Pacific seahorse Hippocampus ingens Girard, 1858
Autor : Marín, Alan
Alfaro, Ruben
Villegas Llerena, Claudio
Reyes Flores, Lorenzo E.
Alvarez Jaque, Irina B.
Robles, Christian
Ingar, Claudia
Yzásiga Barrera, Carmen G.
Calado, Leonardo L.
Zelada Mázmela, Eliana
Fecha de publicación : ago-2021
Resumen : The high demand for syngnathid species in the traditional Chinese medicine (TCM) trade is drastically affecting seahorse populations worldwide. Powdered seahorse not only represents the main material for the preparation of remedies in either TCM or folk medicine but is also being used to facilitate smuggling and avoid customs authorities in different countries. Here, we present the first primer set and standardized real time quantitative PCR (qPCR) protocol designed for a fast, accurate, sensitive, and reliable identification of one of the most threatened fish species in the world, the Pacific seahorse Hippocampus ingens, using DNA traces from powdered material. Our protocol efficiently detected quantities as low as 0.85 pg of seahorse DNA, showing a high specificity even in the presence of DNA from other non-target fish species. The qPCR protocol reported herein may be utilized as a powerful tool to monitor the illegal domestic trade and international trafficking of H. ingens, not only in poaching hotspot countries such as Ecuador and Peru but also in transit and final market destinations such as Hong Kong and China. In addition, we show photographic evidence of seahorse extraction and trade in northern Peru and southern Ecuador, highlighting the need for increased efforts to protect the Pacific seahorse.
Palabras clave : Seahorse
Traditional Chinese medicine
Editorial : ELSEVIER
Citación : Marín, A.; Alfaro, R.; Villegas, C.; Reyes, L.; Alvarez, I.; Robles, C.; Ingar, C.; Yzásiga, C.; Calado, L. & Zelada, E. (2021). Molecular tools against the illegal exploitation of the threatened Pacific seahorse Hippocampus ingens Girard, 1858. Journal for Nature Conservation 62, 126030. https://doi.org/10.1016/j.jnc.2021.126030
Descripción : 12 páginas
URI : https://hdl.handle.net/20.500.12955/1480
metadata.dc.identifier.doi: 10.1016/j.jnc.2021.126030
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