Examinando por Materia "Wheat"
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Ítem Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association(Universidad Politécnica de Valencia, 2015-06-18) Castillo, Ana M.; Sánchez Díaz, Rosa Angélica; Vallés, María P.Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected microspore embryogenesis. By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism. Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25–46% as compared to immature ovaries (excised from flowers with microspores at a mid-late uninucleate stage). The highest numbers of embryos and green plants were produced when using mature Caramba ovaries. Ovaries from Galeón, Tigre, and Kilopondio cultivars successfully induced microspore embryogenesis at the same rate as Caramba ovaries. Moreover, Tigre ovaries raised the percentage of spontaneous chromosome doubling up to 71%. Attempts were made to identify molecular mechanisms associated to the inductive effect of the ovaries on microspore embryogenesis. The genes TAA1b, FLA26, and WALI6 associated to wheat microspore embryogenesis, the CGL1 gene involved in glycan biosynthesis or degradation, and the FER gene involved in the ovary signaling process were expressed and/or induced at different rates during ovary culture. The expression pattern of FLA26 and FER could be related to the differences between genotypes and developmental stages in the inductive effect of the ovary. Our results open opportunities for new approaches to increase bread wheat doubled haploid production by anther culture, and to identify the functional components of the ovary inductive effect on microspore embryogenesis.Ítem Rye production under acid soils and drought conditions: An alternative for the sustainability of High Andean livestock farming in Peru(MDPI, 2023-07-24) Carrasco Chilon, William Leoncio; Alvarez García, Wuesley Yusmein; Cervantes Peralta, Marieta Eliana; Quilcate Pairazamán, Carlos Enrique; Vásquez, Hector V.The rye (Secale cereale L.) crop shows a high potential to contribute to the sustainability of high Andean livestock because it supports the agroclimatic conditions and acid soils in the Peruvian Andes. The production of green forage, hay, and grain from the rye crop in acid soils was studied with the use of different levels of phosphorus and potassium fertilization in four local rye ecotypes (CBI-001, CSM-001, CJS-001, and CCE-001). The green forage yield (GFY) ranged from 32.35 to 53.62 t ha−1, dry matter from 6.05 to 8.56 t ha−1, and hay from 7.0 to 10.36 t ha−1; nutritional levels ranged from 9.02% to 13.56% protein and 6.50% to 7.75% ash levels, mainly with differences between ecotypes (p < 0.05). No differences existed between fertilization levels for the number of stems per plant, spikes per plant, and grains per ear (p > 0.05). Also, CBI-001 and CCE-001 were superior with 1868.4 and 1797.8 kg ha−1 of grain, respectively (p = 0.0072); the use of 60 kg ha of nitrogen, 120 kg ha−1 of P2O5, and 80 kg ha−1 of K2O gave higher grain and residue yields. The high nutritional value and yield of the rye ecotypes studied in acid soil conditions and without irrigation can be an alternative for livestock feeding and grain production in the rainy season in the Andes as a dual-purpose crop.Ítem Rye production under acid soils and drought conditions: an alternative for the sustainability of high Andean livestock farming in Peru(MDPI, 2023-07-05) Carrasco Chilón, William Leoncio; Alvarez García, Wuesley Yusmein; Cervantes Peralta, Marieta Eliana; Quilcate Pairazamán, Carlos Enrique; Vásquez Pérez, Héctor VladimirThe rye (Secale cereale L.) crop shows a high potential to contribute to the sustainability of high Andean livestock because it supports the agroclimatic conditions and acid soils in the Peruvian Andes. The production of green forage, hay, and grain from the rye crop in acid soils was studied with the use of different levels of phosphorus and potassium fertilization in four local rye ecotypes (CBI-001, CSM-001, CJS-001, and CCE-001). The green forage yield (GFY) ranged from 32.35 to 53.62 t ha−1, dry matter from 6.05 to 8.56 t ha−1, and hay from 7.0 to 10.36 t ha−1; nutritional levels ranged from 9.02% to 13.56% protein and 6.50% to 7.75% ash levels, mainly with differences between ecotypes (p < 0.05). No differences existed between fertilization levels for the number of stems per plant, spikes per plant, and grains per ear (p > 0.05). Also, CBI-001 and CCE-001 were superior with 1868.4 and 1797.8 kg ha−1 of grain, respectively (p = 0.0072); the use of 60 kg ha of nitrogen, 120 kg ha−1 of P2O5, and 80 kg ha−1 of K2O gave higher grain and residue yields. The high nutritional value and yield of the rye ecotypes studied in acid soil conditions and without irrigation can be an alternative for livestock feeding and grain production in the rainy season in the Andes as a dual-purpose crop.