Examinando por Autor "Rojas Cruz, Diorman"

Mostrando 1 - 4 de 4

Changes in gut microbiota in peruvian cattle genetic nucleus by breed and correlations with beef quality
(MDPI, 2024-11-29) Quilcate Pairazamán, Carlos Enrique; Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Rojas Cruz, Diorman; Mamani Chullo, Rolando; Hañari Quispe, Renán Dilton; Aliaga, Mery; Galindo, Walter; Vásquez, Héctor V.; Maicelo, Jorge L.; Arbizu, Carlos I.
This study evaluated the gut microbiota and meat quality traits in 11 healthy female cattle from the Huaral region of Peru, including 5 Angus, 3 Braunvieh, and 3 F1 Simmental × Braunvieh. All cattle were 18 months old and maintained on a consistent lifelong diet. Meat quality traits, including loin area, fat thickness, muscle depth, and marbling, were assessed in vivo using ultrasonography. Fecal samples were collected for microbiota analysis, and DNA was extracted for 16S and 18S rRNA sequencing to characterize bacterial, fungal, and protist communities. Significant correlations were observed between microbial genera and meat traits: Christensenellaceae R-7 and Alistipes were positively associated with marbling and muscle area, while Rikenellaceae RC9 showed a negative correlation with fat thickness. Among fungi, Candida positively correlated with marbling, while Trichosporon was negatively associated with muscle depth. For protists, Entodinium negatively correlated with fat thickness and marbling. Alpha diversity varied by breed, with Angus showing greater bacterial diversity, and beta diversity analyses indicated a strong breed influence on microbial composition. These findings suggest that microbial composition, shaped by breed and dietary consistency, could serve as an indicator of meat quality, offering insights into gut microbiota’s role in optimizing cattle production.
Complete mitogenome of “pumpo” (Bos taurus), a top bull from a Peruvian genetic nucleus, and its phylogenetic analysis
(MDPI, 2024-05-28) Estrada Cañari, Richard; Figueroa Venegas, Deyanira Antonella; Romero Avila, Yolanda; Alvarez García, Wuesley Yusmein; Rojas Cruz, Diorman; Alvarado Chuqui, Wigoberto; Maicelo Quintana, Jorge Luis; Quilcate Pairazamán, Carlos Enrique; Arbizu Berrocal, Carlos Irvin
The mitochondrial genome of Pumpo (Bos taurus), a prominent breed contributing to livestock farming, was sequenced using the Illumina HiSeq 2500 platform. Assembly and annotation of the mitochondrial genome were achieved through a multifaceted approach employing bioinformatics tools such as Trim Galore, SPAdes, and Geseq, followed by meticulous manual inspection. Additionally, analyses covering tRNA secondary structure and codon usage bias were conducted for comprehensive characterization. The 16,341 base pair mitochondrial genome comprises 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. Phylogenetic analysis places Pumpo within a clade predominantly composed of European cattle, reflecting its prevalence in Europe. This comprehensive study underscores the importance of mitochondrial genome analysis in understanding cattle evolution and highlights the potential of genetic improvement programs in livestock farming, thus contributing to enhanced livestock practices.
Ganancia de peso vivo de terneros lactantes criados bajo dos sistemas de crianza y alimentación, Molinopampa, Perú
(Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, 2023-01-28) Rojas Cruz, Diorman
El objetivo del presente estudio, consistió en evaluar la ganancia de peso vivo de terneros lactantes de la raza Brown Swiss, criados bajo dos sistemas: crianza y alimentación. La investigación se desarrolló en el distrito de Molinopampa, Amazonas, Perú, desde el 11 de noviembre del 2017 al tres de marzo del 2018, usando 12 terneros en la etapa de lactación, quienes presentaron similares características fenotípicas y genotípicas. Los terneros permitieron evaluar el efecto de las variables: sistema de crianza y sistema de alimentación, sobre los índices productivos, para lo cual se trabajó con un diseño Completamente al azar (DCA), con estructura factorial de tratamientos (2A x 2B). El factor A, estuvo representado por el sistema de crianza y el factor B por el sistema de alimentación. Como resultado se ha determinado que existen diferencias significativas entre las medias de los tratamientos para las variables: ganancia de peso vivo P 0.041 < α 0.05 y conversión alimenticia P 0.030 < α 0.05, obteniendo diferencias altamente significativas para la variable costo de producción P 0.000 < α 0.01. En cambio, para la variable mérito económico, se determinó que no existe diferencias significativas entre tratamientos P 0.097 > α 0.05.
Sex-induced changes in microbial eukaryotes and prokaryotes in gastrointestinal tract of simmental cattle
(MDPI, 2024-11-15) Rojas Cruz, Diorman; Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Figueroa Venegas, Deyanira Antonella; Quilcate Pairazamán, Carlos Enrique; Ganoza Roncal, Jorge Juan; Maicelo Quintana, Jorge Luis; Coila Añasco, Pedro Ubaldo; Alvarado Chuqui, Wigoberto; Cayo Colca, Ilse Silvia
This study investigates gender-based differences in the gut microbiota of Simmental cattle, focusing on bacterial, archaeal, and fungal communities. Fecal samples were collected and analyzed using high-throughput sequencing, with taxonomic classification performed through the SILVA and UNITE databases. Alpha and beta diversity metrics were assessed, revealing significant differences in the diversity and composition of archaeal communities between males and females. Notably, females exhibited higher alpha diversity in archaea, while beta diversity analyses indicated distinct clustering of bacterial and archaeal communities by gender. The study also identified correlations between specific microbial taxa and hematological parameters, with Treponema and Methanosphaera showing gender-specific associations that may influence cattle health and productivity. These findings highlight the importance of considering gender in microbiota-related research and suggest that gender-specific management strategies could optimize livestock performance. Future research should explore the role of sex hormones in shaping these microbial differences.