Examinando por Autor "Quilcate Pairazaman, Carlos Enrique"

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Draft genome sequence and SSR data mining of “pumpo” (Bos taurus), a top bull from a peruvian genetic nucleus
(MDPI, 2024-06-18) Estrada Cañari, Richard; Romero Avila, Yolanda; Figueroa Venegas, Deyanira Antonella; Quilcate Pairazaman, Carlos Enrique; Casanova Nuñez-Melgar, David Pavel; Vásquez Pérez, Hector Vladimir; Alvarado Chuqui, Wigoberto; Maicelo Quintana, Jorge Luis; Arbizu Berrocal, Carlos Irvin
Pumpo is a Simmental breed and an essential livestock resource in the nucleus genetic cattle of Peru. This study provides a draft genome sequence of a top bull using a de novo assembly approach on the Illumina Novaseq X platform, yielding 208 GB of raw sequencing data with 150 bp paired‐end reads. The final genome assembly resulted in a size of 2.06 Gb with an N50 contig length of 108 Mb and a completeness of 95.7% according to BUSCO analysis. A total of 973,925 simple sequence repeats (SSRs) were identified, with a predominance of mononucleotide repeats. The genome showed low heterozygosity (0.568%) and moderate repeatability (11.5%), aligning with other Bos taurus genomes. Reference‐guided scaffolding improved the assembly quality significantly, producing an N50 scaffold value of 108 Mb. The SSR analysis of the Pumpo genome identified 973,925 SSRs with a frequency of 2,808 SSRs per kilobase, predominantly mononucleotide repeats, and 85,453 found in compound formations. Obtaining knowledge of the genome of a breeding Simmental bull is essential to optimize breeding programs and improve productivity.
Season and breed effect in fresh semen parameters at a bull semen production center in Peru
(Unique Scientific Publishers, 2024-07-14) Dipaz Berrocal, Deysi; León Trinidad, Silvia Eliana; Figueroa Venegas, Deyanira Antonella; Mamani Chullo, Rolando; Ramirez Hernandez, Juan; Alvarez García, Wuesley Yusmein; Estrada Cañari, Richard; Quilcate Pairazaman, Carlos Enrique
In the present study, the effect of breed, season, and their interaction on semen volume, sperm concentration, and motility was tested. Nine bulls were tested: three Braunvieh, three Simmental (B. taurus), and three Gyr (B. indicus). These bulls were managed equally in a cold semi-arid climate, divided into hot (from November to April) and cold (from May to October) for maximum and minimum temperature and relative humidity. Semen was collected twice a week for 24 consecutive months. MANOVA by ranks was used to determine significant effects; breed had a significant effect (P<0.001) on semen parameters, and seasonal variations were not significant (P>0.05). However, there was a significant effect (P<0.001) for the interaction between breed and season. To evaluate differences between breeds, we performed Tukey's post-hoc test; all pairwise comparisons (Braunvieh-Gyr, Braunvieh-Simmental and Gyr-Simmental) showed significant differences (P<0.001). Nonparametric one-way analysis showed significantly higher B. taurus breeds’ sperm concentration and B. indicus breed’s semen volume. For interaction between breed and season, Gyr reported higher sperm volume than B. taurus breeds in both seasons (P>0.05), but B. taurus breeds had higher sperm concentration in the cold season. The findings will help management make better decisions in semen collection, especially during peak season.
Sustainability of Lolium multiflorum L. ‘cajamarquino ecotype’, associated with Trifolium repens L., at three cutting frequencies in the Northern Highlands of Peru
(MDPI, 2024-08-13) Vallejos Cacho, Romy; Vallejos Fernández, Luis Asunción; Alvarez García, Wuesley Yusmein; Tapia Acosta, Eduardo Alberto; Saldanha Odriozola, Sylvia; Quilcate Pairazaman, Carlos Enrique
Livestock farming feed in the northern highlands of Peru is based on the association of ryegrass (Lolium multiflorum L.) ecotype cajamarquino–white clover (Trifolium repens L.) Ladino variety, which constantly varies in its agronomic characteristics and nutritional value due to management considerations and its association with the soil and the animal. The objective of this study was to evaluate yield, plant height, growth rate, tillering, tiller number, spikelets, basal diameter in ryegrass, elongation rate, internode length and decline points in clover over one year. Nutritive value was represented by crude protein (CP), neutral detergent fibre (NDF), in vitro digestibility of dry matter (IVDDM) and metabolisable energy (ME) at three cutting frequencies (30, 45 and 60 days). Better yield (5588 kg DM ha) and plant height (47.1 cm) were shown by the 60-day cutting frequency; however, there was no difference (p > 0.05) between the three cutting frequencies in annual yield. There were no differences between the number of tillers and basal diameter. Clover height, elongation rate and internode length were higher at 60 days. The highest CP concentration and the lowest NDF value (p < 0.05) were achieved by clover at 30 and 45 days. Producers should consider the results when deciding when to use this association in dairy cattle feeding.