Examinando por Autor "Montenegro, Juan D."

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Análisis del genoma del cloroplasto del maíz morado INIA 601 para reconstruir la historia evolutiva del maíz morado peruano
(Universidad Nacional Agraria La Molina, 2019-10) Rodríguez Pérez, Lila M.; Montenegro, Juan D.; Simon, Reinhard; Chumbe Nolasco, Lenin; Serna Chumbes, Manuel Fernando; Delgado, Gabriel; García Serquén, Aura Liz; Gutiérrez Reynoso, Dina Lida
En el presente estudio, se ha reconstruido la secuencia completa del genoma plastidial del maíz morado peruano y se ha comparado con otros genomas plastidiales de maíces. El genoma plastidial tiene una longitud de 140,458 pb y muestra una estructura típica del genoma del cloroplasto: un par de regiones repetidas invertidas (IRa e IRb) de 22,594 pb, una región Larga de Copia Única (LSC) de 82,472 pb, y una región Corta de Copia Única (SSC) de 12,798 pb. Las relaciones filogenéticas fueron obtenidas a partir de alineamientos genómicos completos con los genomas plastidiales de otros miembros del género Zea. Los resultados indicaron que el maíz morado peruano está más relacionado a Z. mays subsp. huehuetenangensis. Es posible que eventos de hibridación introgresiva a lo largo de la evolución del maíz morado hayan jugado un papel en la adquisición del fenotipo morado en el clado Z. mays.
Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
(Universidad Nacional de Trujillo, 2021-03-10) Gutiérrez Calle, Savina Alejandra; Sánchez Díaz, Rosa A.; Delgado Silva, Yolanda Bedsabé; Montenegro, Juan D.; Gutiérrez Reynoso, Dina Lida; Maicelo Quintana, Jorge Luis; Guerrero Abad, Juan Carlos
Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated.