Examinando por Autor "Meza Maicelo, Wagner"

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Identification of Metamasius hemipterus (Coleoptera: Dryophthoridae) and its biological control through the use of species of the genus Beauveria and Metarhizium. Amazonas, Peru
(Frontiers Media S.A, 2026-01-12) Leiva Espinoza, Santos Triunfo; Román Peña, Alcides; Aguilar, Vilma; Requejo, Elizabeth; Meza Maicelo, Wagner; Banda Martinez, Duber; Huaman Pilco, Ángel Fernando; Loyola , Fredy; Loja, Melissa; Oliva Cruz, Manuel; Maicelo Quintana, Jorge
Biological control of insect pests represents a key strategy toward sustainable agriculture. This study, focused on the morphological and molecular identification of the sugarcane weevil, Metamasius hemipterus, (Coleoptera: Dryophthoridae), and on the evaluation of the biocontrol potential of four entomopathogenic fungi: Beauveria bassiana, Metarhizium anisopliae, Beauveria peruviensis, and Metarhizium sp. Molecular identification was conducted through phylogenetic analysis, while morphological identification was based on the description of characteristics following established protocols. Both approaches confirmed the identity of the pest as Metamasius hemipterus L. Bioassays were carried out under a completely randomized design, testing four conidial concentrations, plus a control. Metarhizium anisopliae at 1 × 10¹⁰ conidia/mL was the most virulent, producing the highest mortality and exhibiting the lowest lethal concentration (LC₅₀ = 2.68 × 10⁹ conidia/mL) against Metamasius hemipterus adults. Conversely, Beauveria peruviensis at 1 × 10¹⁴ conidia/mL achieved the highest colonization efficiency in insect cadavers, with a mycosis rate of 99.4%. These findings indicate that Beauveria peruviensis and Metarhizium anisopliae are promising biological control agents against the sugarcane weevil, the major pest of sugarcane in the Amazonas region of Peru.
Microbial bio-inoculation effects on the seed germination dynamics and field performance of pea (Pisum sativum L.) under osmotic stress and fertilization in the Amazonas region of Peru
(MDPI, 2026-04-10) Guevara Fernández, Francisco; Casas Niño, Sebastian Antonio; Munoz Salas, Milagros Ninoska; Meza Maicelo, Wagner; Oliva Cruz, Manuel; Lozano Isla, Flavio
Microbial bio-inoculants have been proposed as management tools to enhance crop performance under variable environmental conditions; however, their effectiveness is often influenced by site-specific factors. This study evaluated the effects of bio-inoculation on seed germination and seedling vigor of pea under osmotic stress induced by polyethylene glycol (PEG 6000), and its interaction with two fertilization levels (75% and 100% of the recommended dose) under field conditions in the Amazonas region of Peru. Under laboratory conditions, germination percentage remained high across all treatments (93.3–100%) and was not affected by bio-inoculation or osmotic potential; however, osmotic stress altered germination dynamics, increasing mean germination time from 1.85–2.09 days at 0 MPa to 2.26–2.43 days at −0.8 MPa, while germination synchrony and seedling vigor decreased as stress increased. The seedling vigor index reached maximum values at −0.2 MPa (4.47–5.29) and declined at −0.8 MPa (1.50–2.00), and multivariate analyses showed that variation in germination responses was mainly associated with germination timing and vigor rather than seed viability. Under field conditions, no significant effects of fertilization level, microbial bio-inoculation, or their interaction were detected on agronomic traits or yield, although variability between locations was observed; plant height ranged from 38.5–46.3 cm in Lamud and from 100.6–108.3 cm in Molinopampa, while grain yield varied from 698–1846 kg/ha and 8771–9919 kg/ha, respectively. Overall, environmental conditions exerted a stronger influence than microbial bio-inoculation on germination dynamics and field productivity, while the findings provide practical guidance for improving pea production with bio-inoculants and optimized fertilization.