Examinando por Autor "León Trinidad, Silvia Eliana"

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Age-dependent changes in protist and fungal microbiota in a peruvian cattle genetic nucleus
(MDPI, 2024-08-14) Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Quilcate Pairazamán, Carlos Enrique; Dipaz Berrocal, Deisy Juana; Alejos Asencio, Carol Silvia; León Trinidad, Silvia Eliana; Alvarez García, Wuesley Yusmein; Rojas, Diorman; Alvarado Chuqui, Wigoberto; Maicelo Quintana, Jorge Luis; Arbizu, Carlos Irvin
In this research, the connection between age and microbial diversity in cattle was explored, revealing significant changes in both protist diversity and fungal microbiota composition with age. Using fecal samples from 21 Simmental cattle, microbial communities were analyzed through 18S rRNA gene sequencing. Results indicated significant differences in alpha protist diversity among the three age groups, while fungal composition varied notably with age and was linked to hematological parameters. Despite the stability of fungal alpha diversity, compositional changes suggest the gut as a stable niche for microbial colonization influenced by diet, clinical parameters, and microbial interactions. All cattle were maintained on a consistent diet, tailored to meet the specific nutritional needs of each age group. These findings emphasize the importance of understanding age-related microbial dynamics to enhance livestock management and animal health, contributing to broader ecological and biomedical research. This study was limited by the lack of comprehensive metabolic analyses correlating microbiota changes with specific age-related variations, indicating a need for further research in this área.
Complete mitogenome, phylogenetic and SNP-data analysis of jakumaru (Bos taurus), a prime bull from a Peruvian breeding core herd
(Unique Scientific Publishers, 2025-01-22) Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Figueroa Venegas, Deyanira Antonella; Quilcate Pairazamán, Carlos Enrique; León Trinidad, Silvia Eliana; Casanova Nuñez Melgar, David pavel; Maicelo, Jorge L.; Arbizu, Carlos I.
This study provides a comprehensive mitochondrial genome sequence and a phylogenetic analysis of Jakumaru (Bos taurus), a prominent bull from a Peruvian breeding program. The mitochondrial genome, spanning 16,340bp, comprises 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes, with a GC content of 40%. The gene arrangement and structural organization are highly conserved, closely resembling those of related Bovine species, indicating a stable genomic architecture essential for mitochondrial function. Phylogenetic analysis, incorporating 35 complete bovine mitochondrial genomes, positions Jakumaru within Subclade I, revealing a close relationship with European Simmental cattle. The study emphasizes the biological diversity and the evolution of the Simmental breed, influenced by natural selection, breeding practices, and genetic drift. These findings enhance our understanding of the breed's genetic variation and its evolution, particularly in the context of its importance in Peru
Effect of protein source and breed on embryo production in donor cows at a high-value genetic centre in Peru
(Unique Scientific Publishers, 2025-04-23) León Trinidad, Silvia Eliana; Dipaz Berrocal, Deisy Juana; Mamani, Rolando; Ramírez, Juan; Muñoz Vilchez, Yudith Yohany; Burga Toledo, Henry; Quilcate Pairazaman, Carlos Enrique; Alvarez García, Wuesley Yusmein
Two sources of concentrated animal protein at 50 and 60% of the diet of high genetic value cows were evaluated on embryo production in donor cows. Twenty Braunvieh (BU), Brahman (BR), Simmental (SM), Gir (G) and Girolando (GIHO) cows were evaluated for seven months. The SM breed achieved a higher number of corpora lutea, similar to Brahman cows, but no difference was shown between the two protein rations. It was determined that using both protein sources decreases production costs per feeding, with the same results in embryo production. This study emphasizes the great importance of adequate protein nutrition in embryo transfer programs to maximize reproductive efficiency and embryo viability, taking into account the welfare of the animals subjected to these reproductive evaluations, in order to improve productivity in a center for the production and reproduction of material or germplasm of high genetic value.
Season and breed effect in fresh semen parameters at a bull semen production center in Peru
(Unique Scientific Publishers, 2024-07-14) Dipaz Berrocal, Deysi; León Trinidad, Silvia Eliana; Figueroa Venegas, Deyanira Antonella; Mamani Chullo, Rolando; Ramirez Hernandez, Juan; Alvarez García, Wuesley Yusmein; Estrada Cañari, Richard; Quilcate Pairazaman, Carlos Enrique
In the present study, the effect of breed, season, and their interaction on semen volume, sperm concentration, and motility was tested. Nine bulls were tested: three Braunvieh, three Simmental (B. taurus), and three Gyr (B. indicus). These bulls were managed equally in a cold semi-arid climate, divided into hot (from November to April) and cold (from May to October) for maximum and minimum temperature and relative humidity. Semen was collected twice a week for 24 consecutive months. MANOVA by ranks was used to determine significant effects; breed had a significant effect (P<0.001) on semen parameters, and seasonal variations were not significant (P>0.05). However, there was a significant effect (P<0.001) for the interaction between breed and season. To evaluate differences between breeds, we performed Tukey's post-hoc test; all pairwise comparisons (Braunvieh-Gyr, Braunvieh-Simmental and Gyr-Simmental) showed significant differences (P<0.001). Nonparametric one-way analysis showed significantly higher B. taurus breeds’ sperm concentration and B. indicus breed’s semen volume. For interaction between breed and season, Gyr reported higher sperm volume than B. taurus breeds in both seasons (P>0.05), but B. taurus breeds had higher sperm concentration in the cold season. The findings will help management make better decisions in semen collection, especially during peak season.
Seroprevalence of reproductive and infectious diseases in cattle: the case of Madre de Dios in the Peruvian southeastern tropics
(American Veterinary Medical Association, 2024-02-12) León Trinidad, Silvia Eliana; Barrantes Bravo, Christian; Feijoo Narvasta, Shefferson Gilbert Wilson; Huamán Fuertes, Ethel; Ampuero Trigoso, Gustavo; Canto Sáenz, Francys Mitchel; Quispe Ccasa, Hurley Abel
OBJECTIVE The objective of this study was to determine the seroprevalence of reproductive and infectious diseases in tropical cattle in the Tambopata and Tahuamanu Provinces in the department of Madre de Dios, Peru. SAMPLE 156 bovines from 7 cattle farms were sampled. These farms used exclusive grazing for food and natural mating for reproduction and did not have sanitary or vaccination programs. METHODS The serum of blood samples was subjected to ELISA with commercial kits for the detection of antibodies against Neospora caninum, Mycobacterium avium subsp paratuberculosis (MAP), Leptospira interrogans, pestivirus bovine viral diarrhea virus-1, retrovirus bovine leukemia virus (BLV), orbivirus bluetongue virus (BTV), and herpesvirus bovine herpes virus-1 (BHV). The data were analyzed by means of association tests with χ2 (P < .05) and Spearman rank correlation (P < .05) in the SPSS v.15.0 software (IBM Corp). RESULTS A low prevalence of antibodies to L interrogans, N caninum, M avium subsp paratuberculosis, bovine viral diarrhea virus-1 was found, but it was high to BTV, BLV, and BHV (100%, 53.85%, and 72.44%, respectively). The presence of BLV and BHV was higher in the Las Piedras District, bovines less than 5 years old, and cattle with breed characteristics of zebu and crossbred (P < .01). In addition, there was a significant correlation between both infections, showing 83.3% of BLV positivity that were also BHV positive (P < .01). CLINICAL RELEVANCE The high prevalence of antibodies to BTV, BHV, and BLV could be due to livestock management practices, direct con tact with infected animals, and variation of the presence of vectors and natural reservoirs in the context of climate change in the tropics.