Examinando por Autor "Huaman Pilco, Angel Fernando"
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Ítem Integrating agroecological suitability of cacao (Theobroma cacao L.) with biodiversity and land-use constraints in Peru(Elsevier Ltd., 2026-01-29) Cotrina Sanchez, Alexander; Guzman Valque, Betty Karina; Barboza, Elgar; Oliva, Manuel; Huaman Pilco, Angel Fernando; Rojas Briceño, Nilton B.CONTEXT: Cacao cultivation is vital for rural economies in Peru, but its expansion often overlaps with sensitive ecosystems, raising concerns for biodiversity conservation. Despite international commitments to deforestation-free supply chains, integrated analyses combining agroecological suitability with land-use constraints remain scarce in Peru. OBJECTIVES: This study aims to identify suitable areas for cacao cultivation under multiple exclusion scenarios, evaluate conflicts with biodiversity and conservation areas, and quantify degraded lands that could provide opportunities for agroforestry-based restoration. METHODS: Cacao suitability was modelled with an ensemble of nine machine-learning algorithms using bioclimatic, edaphic, and topographic predictors. Outputs were filtered to exclude biophysical barriers and overlaid with national-scale layers of species richness, protected areas, forest cover, and degraded lands through GIS-based spatial analysis to evaluate exclusion scenarios and trade-offs. RESULTS AND CONCLUSIONS: The ensemble achieved high predictive power, with Random Forest (AUC = 0.997) and XGBoost (AUC = 0.972) performing best. Highly suitable areas were concentrated in the Andean-Amazon transition, especially in San Martín, Cusco, Huánuco, and Junín departments, where they overlapped with biodiversity hotspots and legally protected areas. Degraded yet suitable lands highlighted opportunities to expand cacao through agroforestry systems, reducing forest pressure and enhancing ecological restoration. SIGNIFICANCE: By integrating suitability modelling with national-scale geospatial layers, this study delivers a framework linking crop suitability with land-use constraints. The findings support national-scale planning while remaining adaptable to local contexts. They also align with international policy frameworks such as the European Deforestation Regulation (EUDR), promoting sustainable cacao production, biodiversity conservation, and long-term rural development in Peru.Ítem Micropropagation of Vaccinium meridionale Sw.: interaction between basal media and cytokinins, physiological quality of shoots, and ex vitro rooting(Elsevier Inc. on behalf of Academy of Scientific Research and Technology, 2025-12-22) Huaman, Eyner; Muñoz, Carlos; Prat, Loreto; Meléndez Mori, Jegnes Benjamín; Vargas, Raúl; Vigo, Carmen; Tejada Alvarado, José Jesús; Huaman Pilco, Angel Fernando; Oliva Cruz, ManuelVaccinium meridionale is an Andean species of high nutraceutical value whose conventional propagation is limited by its low multiplication rate. In this study, an integrated micropropagation protocol was developed, encompassing in vitro establishment through ex vitro rooting. During the establishment phase, fungal contaminants were identified, detecting genera such as Diaporthe, Fusarium, Colletotrichum and Phoma. In the multiplication phase, the basal media Driver and Kuniyuki (DKW), Woody Plant Medium (WPM) and Murashige and Skoog (MS) were evaluated, supplemented with zeatin (Zea), 2-isopentenyladenine (2iP), meta-topolin (mT) and thidiazuron (TDZ), all applied at equimolar concentrations of 2.5 μM. Morphogenic parameters, photosynthetic pigment content, SPAD index and elemental composition of regenerated tissues were quantified. DKW medium supplemented with Zea or 2iP promoted the formation of 9–10 shoots, with lengths of 2.5–2.9 cm, SPAD values of 35–36, and chlorophyll content >31 μg/mL. The accumulation of P, Ca, Mg, Fe, Zn, Cu and Mn in the tissues varied widely among treatments. During rooting, the application of 500 ppm naphthaleneacetic acid (NAA) induced the formation of longer roots, as well as vigorous and elongated shoots with a high number of leaves. This optimized protocol provides a valuable tool for the propagation of V. meridionale, with potential applications in conservation, genetic improvement and commercial plant production.
