Examinando por Autor "Gutiérrez Reynoso, Dina Lida"

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Agro-morphological characterization and diversity analysis of Coffea arabica germplasm collection from INIA, Peru
(John Wiley & Sons Inc., 2023-04-04) Paredes Espinosa, Richard; Gutiérrez Reynoso, Dina Lida; Atoche Garay, Diego Fernando; Abad Romaní, Yudi Gertrudis; Girón Aguilar, Rita Carolina; Flores Torres, Itala; Montañez Artica, Ana Gabriela; Arbizu Berrocal, Carlos Irvin; Amasifuen Guerra, Carlos Alberto; Poemape Tuesta, Carlos Augusto; Guerrero Abad, Juan Carlos
Coffee (Coffea arabica L.) plays a major role in the economy of Peru and the world. The present study aims to elucidate the agro-morphological variability of coffee genotypes maintained in the INIA´s germplasm collection. Therefore, 20 vegetative, reproductive, and phytosanitary traits of 162 coffee accessions of INIA’s germplasm collection were evaluated and analyzed. Correlation results indicate that a simultaneous selection of characters, such as number of branches per plant, number of nodes per branch, leaf area and weight of a hundred fruits, can contribute to increase coffee yields. Additionally, coffee yield was negatively correlated with the incidence and severity of coffee leaf rust, and interestingly the occurrence of small and compact coffee plants with high resistance to the disease was also found. The analysis of Tocher and Mahalanobis D2 determined the formation of 10 groups of divergent coffee accessions; where clusters 1 (accession codes 20, 29, 38, 54, 67, 71, 117, 24, 26 and 27), 5 (accession codes 46 and 53), 9 (accession code 159), and 10 (accession code 203) group promising accessions that can be used in breeding programs. Principal component analysis (PCA) showed that at least five of its principal components managed to explain 70.01% of the total variation in the collection. Finally, the high coefficients obtained for the phenotypic, genotypic and heritability variation confirm the existence of additive genes in the evaluated population, that would ensure the success of coffee breeding programs based on the selection of traits of agronomic importance.
Agro-morphological characterization and diversity analysis of Coffea arabica germplasm collection from INIA, Peru
(John Wiley & Sons Inc., 2023-06-06) Paredes Espinosa, Richard; Gutiérrez Reynoso, Dina Lida; Atoche Garay, Diego Fernando; Mansilla Córdova, Pedro Javier ; Abad Romaní, Yudi Gertrudis; Girón Aguilar, Rita Carolina; Flores Torres, Itala; Montañez Artica, Ana Gabriela; Arbizu Berrocal, Carlos Irvin; Amasifuen Guerra, Carlos Alberto; Maicelo Quintana, Jorge Luis; Poemape Tuesta, Carlos Augusto; Guerrero Abad, Juan Carlos
Coffee (Coffea arabica L.) plays a major role in the economy of Peru and the world. The present study aims to elucidate the agro-morphological variability of coffee genotypes maintained in the INIA´s germplasm collection. Therefore, 20 vegetative, reproductive, and phytosanitary traits of 162 coffee accessions of INIA’s germplasm collection were evaluated and analyzed. Correlation results indicate that a simultaneous selection of characters, such as number of branches per plant, number of nodes per branch, leaf area and weight of a hundred fruits, can contribute to increase coffee yields. Additionally, coffee yield was negatively correlated with the incidence and severity of coffee leaf rust, and interestingly the occurrence of small and compact coffee plants with high resistance to the disease was also found. The analysis of Tocher and Mahalanobis D2 determined the formation of 10 groups of divergent coffee accessions; where clusters 1 (accession codes 20, 29, 38, 54, 67, 71, 117, 24, 26 and 27), 5 (accession codes 46 and 53), 9 (accession code 159), and 10 (accession code 203) group promising accessions that can be used in breeding programs. Principal component analysis (PCA) showed that at least five of its principal components managed to explain 70.01% of the total variation in the collection. Finally, the high coefficients obtained for the phenotypic, genotypic and heritability variation confirm the existence of additive genes in the evaluated population, that would ensure the success of coffee breeding programs based on the selection of traits of agronomic importance.
Análisis del genoma del cloroplasto del maíz morado INIA 601 para reconstruir la historia evolutiva del maíz morado peruano
(Universidad Nacional Agraria La Molina, 2019-10) Rodríguez Pérez, Lila M.; Montenegro, Juan D.; Simon, Reinhard; Chumbe Nolasco, Lenin; Serna Chumbes, Manuel Fernando; Delgado, Gabriel; García Serquén, Aura Liz; Gutiérrez Reynoso, Dina Lida
En el presente estudio, se ha reconstruido la secuencia completa del genoma plastidial del maíz morado peruano y se ha comparado con otros genomas plastidiales de maíces. El genoma plastidial tiene una longitud de 140,458 pb y muestra una estructura típica del genoma del cloroplasto: un par de regiones repetidas invertidas (IRa e IRb) de 22,594 pb, una región Larga de Copia Única (LSC) de 82,472 pb, y una región Corta de Copia Única (SSC) de 12,798 pb. Las relaciones filogenéticas fueron obtenidas a partir de alineamientos genómicos completos con los genomas plastidiales de otros miembros del género Zea. Los resultados indicaron que el maíz morado peruano está más relacionado a Z. mays subsp. huehuetenangensis. Es posible que eventos de hibridación introgresiva a lo largo de la evolución del maíz morado hayan jugado un papel en la adquisición del fenotipo morado en el clado Z. mays.
Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
(Universidad Nacional de Trujillo, 2021-03-10) Gutiérrez Calle, Savina Alejandra; Sánchez Díaz, Rosa A.; Delgado Silva, Yolanda Bedsabé; Montenegro, Juan D.; Gutiérrez Reynoso, Dina Lida; Maicelo Quintana, Jorge Luis; Guerrero Abad, Juan Carlos
Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated.
Characterization of the complete mitochondrial genome of the black Alpaca breed of Vicugna pacos (Mammalia, Artiodactyla, Camelidae) from Puno, Peru
(Taylor & Francis Group, 2020-03-09) Bustamante, Danilo E.; Yalta Macedo, Claudia Esther; Cruz Luis, Juancarlos Alejandro; Maicelo Quintana, Jorge Luis; Guerrero Abad, Juan Carlos; Gutiérrez Reynoso, Dina Lida
The domestic South American camelid Vicugna pacos L. is distributed along Peru, Chile, Bolivia, and Argentina. Here, we contribute to the bioinformatics and evolutionary systematics of the Camelidae by performing high-throughput sequencing analysis on the black Huacaya breed of V. pacos from Puno, Peru. The black Huacaya breed mitogenome is 16,664 base pairs (bp) in length and contains 37 genes (GenBank accession MT044302). The mitogenome shares a high-level of gene synteny to other Camelidae (Camelops, Camelus, Lama, and Vicugna). The mitogenome of the black Huacaya breed of V. pacos situates it in a clade with V. vicugna Molina, sister to Lama. We anticipate that further mitogenome sequencing of different breeds from Vicugna pacos will improve our understanding of the evolutionary history of this taxon.
Characterization of the horizontal resistance of coffee (Coffea arabica) to yellow rust (Hemileia vastatrix) in the field and laboratory
(The American Phytopathological Society, 2022-03-25) Paredes Espinosa, Richard; Mansilla Córdova, P. J.; Abad Romaní, Yudi Gertrudis; Gutiérrez Reynoso, Dina Lida; Guerrero Abad, Juan Carlos; Egoávil, G.
Between 2011–2013, yellow rust unleashed one of the most important phytosanitary crises in the coffee sector in Peru, causing a negative impact on the rural family economy. One of the factors associated with the intensification of the disease was the predominance of susceptible cultivars in agricultural systems. Faced with the need to rehabilitate the coffee park with new cultivars, the National Institute for Agrarian Innovation (INIA) has managed the installation of basic coffee germplasm for the development of the yellow rust resistance program, which is made up of 169 introductions from the departments of Pasco, Junín, Huánuco, Ucayali, Cajamarca, and Amazonas and obtained from 2015. The objective of the study was to evaluate the incidence and severity of epidemic conditions in the field and characterize the components of horizontal resistance, using the method of inoculations of H. vastatrix in separated coffee leaves in the laboratory. The epidemiological results collected from the field indicate that 12.43 and 20.71% of the genotypes showed complete and incomplete resistance, respectively; 66.86% showed high susceptibility to the pathogen. Three of the outstanding genotypes gave a typical horizontal resistance reaction when artificially inoculated with uredinospores on separated coffee leaves kept in a humid chamber.
Draft Genome Sequence of Bacillus thuringiensis Strain UNMSM10RA, Isolated from Potato Crop Soil in Peru
(American Society for Microbiology, 2020-01-09) Delgado Silva, Yolanda Bedsabé; Tarazona, David; Serna Chumbes, Manuel Fernando; Juscamayta, Eduardo; Chávez Galarza, Julio César; Farfán Vignolo, Evelyn Roxana; Delgado, Gabriel; Flores, Abad; Solano, Gabriela; Gutiérrez Reynoso, Dina Lida
The 5.5-Mb genome sequence of Bacillus thuringiensis strain UNMSM10RA, isolated from potato crop soil, is reported in this study. The strain UNMSM10RA contains 5,347 protein-coding sequences, 105 tRNA genes, 15 rRNA genes, and 5 noncoding RNA (ncRNA) genes, with an average G+C content of 35.1%. Within protein-coding genes, 31 were detected and identified in the metabolism of heavy metals such as arsenic, cadmium, and copper. Further analyses will be performed and provide more information for understanding the evolution of these Bacillus thuringiensis strains and their potential uses.
Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings
(Universidad Nacional de Trujillo, 2023-07-10) Hernández Amasifuen, Angel David; Rivadeneyra Chisquipama, Lenin; Padilla Dominguez, Amner; Paredes Espinosa, Richard; Guerrero Abad, Juan Carlos; Torres Flores, Elías; Peláez Rivera, Jorge Luis; Carvajal Vallejos, Fernando Marcelo; Gutiérrez Reynoso, Dina Lida; Corazon Guivin, Mike Anderson
Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the multiplication process of H. vastatrix uredospores, using controlled conditions (23±1 °C, ≈ 80% relative humidity and photoperiod of 16 hours of light) under an innovative system, the first symptoms of chlorosis were observed on coffee leaves 20 days after inoculation. Our findings show a 100% incidence of inoculated coffee plants with an average of 54 mg of H. vastatrix uredospores per plant and a 1.38 x 10-1 mg/cm2 sporulation rate. Finally, here we report a protocol that allows mass multiplication of H. vastatrix uredospores under controlled conditions, that would be useful in any time for coffee leaf rust bioassays.
Mutations found in the Asc1 gene that confer susceptibility to the AAL-Toxin in ancestral tomatoes from Peru and Mexico
(MDPI, 2020-12-28) Tsuzuki, Rin; Cabrera Pintado, Rosa María; Biondi Thorndike, Jorge Andrés; Gutiérrez Reynoso, Dina Lida; Amasifuen Guerra, Carlos Alberto; Guerrero Abad, Juan Carlos; Aragón Caballero, Liliana María; Huarhua Zaquinaula, Medali Heidi; Ureta Sierra, Cledy; Alberca Cruz, Olenka Ines; Elespuru Suna, Milca Gianira; Blas Sevillano, Raúl Humberto; Torres Arias, Ines Carolina; Flores Ticona, Joel; Cáceres de Baldárrago, Fátima; Rodoríguez Pérez, Enrique; Hozum, Takuo; Saito, Hiroki; Kotera, Shunsuke; Akagi, Yasunori; Kodama, Motoichiro; Komatsu, Ken; Arie, Tsutome
Tomato susceptibility/resistance to stem canker disease caused by Alternaria alternata f. sp. lycopersici and its pathogenic factor AAL-toxin is determined by the presence of the Asc1 gene. Several cultivars of commercial tomato (Solanum lycopersicum var. lycopersicum, SLL) are reported to have a mutation in Asc1, resulting in their susceptibility to AAL-toxin. We evaluated 119 ancestral tomato accessions including S. pimpinellifolium (SP), S. lycopersicum var. cerasiforme (SLC) and S. lycopersicum var. lycopersicum “jitomate criollo” (SLJ) for AAL-toxin susceptibility. Three accessions, SP PER018805, SLC PER018894, and SLJ M5-3, were susceptible to AAL-toxin. SLC PER018894 and SLJ M5-3 had a two-nucleotide deletion (nt 854_855del) in Asc1 identical to that found in SLL cv. Aichi-first. Another mutation (nt 931_932insT) that may confer AAL-toxin susceptibility was identified in SP PER018805. In the phylogenetic tree based on the 18 COSII sequences, a clade (S3) is composed of SP, including the AAL-toxin susceptible PER018805, and SLC. AAL-toxin susceptible SLC PER018894 and SLJ M5-3 were in Clade S2 with SLL cultivars. As SLC is thought to be the ancestor of SLL, and SLJ is an intermediate tomato between SLC and SLL, Asc1s with/without the mutation seem to have been inherited throughout the history of tomato domestication and breeding.
Phylogenomic Analysis of the Plastid Genome of the Peruvian Purple Maize Zea mays subsp. mays cv. ‘INIA 601’
(MDPI, 2022-10-15) Montenegro Cabrera, Juan Daniel; Julca Chavez, Irene Consuelo; Chumbe Nolasco, Lenin Dimitriv; Rodríguez Pérez, Lila Maciel; Sevilla Panizo, Ricardo; Medina Hoyos, Alicia Elizabeth; Gutiérrez Reynoso, Dina Lida; Guerrero Abad, Juan Carlos; Amasifuen Guerra, Carlos Alberto; García Serquén, Aura Liz
Peru is an important center of diversity for maize; its different cultivars have been adapted to distinct altitudes and water availability and possess an array of kernel colors (red, blue, and purple), which are highly appreciated by local populations. Specifically, Peruvian purple maize is a collection of native landraces selected and maintained by indigenous cultures due to its intense purple color in the seed, bract, and cob. This color is produced by anthocyanin pigments, which have gained interest due to their potential use in the food, agriculture, and pharmaceutical industry. It is generally accepted that the Peruvian purple maize originated from a single ancestral landrace ‘Kculli’, but it is not well understood. To study the origin of the Peruvian purple maize, we assembled the plastid genomes of the new cultivar ‘INIA 601’ with a high concentration of anthocyanins, comparing them with 27 cultivars/landraces of South America, 9 Z. mays subsp. parviglumis, and 5 partial genomes of Z. mays subsp. mexicana. Using these genomes, plus four other maize genomes and two outgroups from the NCBI database, we reconstructed the phylogenetic relationship of Z. mays. Our results suggest a polyphyletic origin of purple maize in South America and agree with a complex scenario of domestication with recurrent gene flow from wild relatives. Additionally, we identify 18 plastid positions that can be used as high-confidence genetic markers for further studies. Altogether, these plastid genomes constitute a valuable resource to study the evolution and domestication of Z. mays in South America.
Rapid plant DNA and RNA extraction protocol using a bench drill
(FUNPEC-RP, 2019-08-14) Ferreira, C.F; Gutiérrez Reynoso, Dina Lida; Kreuze, Jan; Iskra Caruana, M.L; Chabannes, M; Barbosa, A.C.O; Santos, T.A; Silva, A.G.S; Amorim, E.P; De Oliveira, S.A.S; Jesus, O.N
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
Traditional potato tillage systems in the Peruvian Andes impact bacterial diversity, evenness, community composition, and functions in soil microbiomes
(Nature Publishing Group, 2024-02-17) García Serquén, Aura Liz; Chumbe Nolasco, Lenin Dimitriv; Navarrete, Acacio Aparecido; Girón Aguilar, Rita Carolina; Gutiérrez Reynoso, Dina Lida
The soil microbiome, a crucial component of agricultural ecosystems, plays a pivotal role in crop production and ecosystem functioning. However, its response to traditional tillage systems in potato cultivation in the Peruvian highlands is still far from understood. Here, ecological and functional aspects of the bacterial community were analyzed based on soil samples from two traditional tillage systems: 'chiwa' (minimal tillage) and 'barbecho' (full tillage), in the Huanuco region of the Peruvian central Andes. Similar soil bacterial community composition was shown for minimal tillage system, but it was heterogeneous for full tillage system. This soil bacterial community composition under full tillage system may be attributed to stochastic, and a more dynamic environment within this tillage system. 'Chiwa' and 'barbecho' soils harbored distinct bacterial genera into their communities, indicating their potential as bioindicators of traditional tillage effects. Functional analysis revealed common metabolic pathways in both tillage systems, with differences in anaerobic pathways in 'chiwa' and more diverse pathways in 'barbecho'. These findings open the possibilities to explore microbial bioindicators for minimal and full tillage systems, which are in relationship with healthy soil, and they can be used to propose adequate tillage systems for the sowing of potatoes in Peru.
Uso de maracuyá (Passiflora edulis f. flavicarpa) como portainjerto para la propagación de especies de Passiflora supersect. Tacsonia colectadas en Perú
(Universidad Nacional Agraria La Molina, Facultad de Ciencias Forestales, 2024-02-05) Chávez Corcuera, Gonzalo; Torres Chacón, Rosmery; Fernandez Huaytalla, Elizabeth; Elías Da Silva, Rosa Angelica; Gutiérrez Reynoso, Dina Lida
Las especies de Passiflora supersect. Tacsonia (Juss.) Feuillet & J.M. MacDougal son plantas adaptadas a los ecosistemas andinos que producen frutos comestibles. Estos son cultivados y comercializados a pequeña escala para su consumo como fruta fresca y para la elaboración de postres y bebidas. Ante las fuertes perturbaciones antrópicas y ambientales que sufre el hábitat natural de las especies de Passiflora L., la extinción parcial o total de ellas es una realidad latente. Por ello, con el fin de conservar estos recursos en Bancos de Germoplasma, es necesario el desarrollo de métodos óptimos para la propagación de las distintas especies. Con la intención de conservar ex situ, en la Colección de Passiflora del Banco de Germoplasma del Instituto Nacional de Innovación Agraria (INIA) a las especies de Passiflora supersect. Tacsonia, se verificó la viabilidad del uso de Passiflora edulis f. flavicarpa O. Deg. como portainjerto para la propagación asexual de seis taxa seleccionados. Passiflora tripartita var. mollissima (Kunth) Holm-Niels. & P. Jørg., P. cumbalensis (H. Karst.) Harms, P. pinnatistipula Cav., P. tarminiana Coppens & V.E. Barney, P. peduncularis Cav. y P. trifoliata Cav. var. trifoliata fueron injertados en P. edulis f. flavicarpa obteniendo un éxito de prendimiento y aclimatación óptima para todos los taxa seleccionados. Adicionalmente, todos los taxa con excepción de P. trifoliata var. trifoliata produjeron flores y frutos. Finalmente, este es el primer registro de propagación y cultivo exitoso para P. trifoliata y P. peduncularis, ambas especies endémicas de Perú, en un hábitat cercano al nivel del mar.