Examinando por Autor "Gonzales, Jhony"
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Ítem Cover and land use changes in the dry forest of Tumbes (Peru) using sentinel-2 and google earth engine data(MDPI, 2022-10-21) Barboza Castillo, Elgar; Salazar Coronel, Wilian; Gálvez Paucar, David; Valqui Valqui, Lamberto; Saravia Navarro, David; Gonzales, Jhony; Aldana, Wiliam; Vásquez Pérez, Héctor Vladimir; Arbizu Berrocal, Carlos IrvinDry forests are home to large amounts of biodiversity, are providers of ecosystem services, and control the advance of deserts. However, globally, these ecosystems are being threatened by various factors such as climate change, deforestation, and land use and land cover (LULC). The objective of this study was to identify the dynamics of LULC changes and the factors associated with the transformations of the dry forest in the Tumbes region (Peru) using Google Earth Engine (GEE). For this, the annual collection of Sentinel 2 (S2) satellite images of 2017 and 2021 was analyzed. Six types of LULC were identified, namely urban area (AU), agricultural land (AL), land without or with little vegetation (LW), water body (WB), dense dry forest (DDF), and open dry forest (ODF). Subsequently, we applied the Random Forest (RF) method for the classification. LULC maps reported accuracies greater than 89%. In turn, the rates of DDF and ODF between 2017 and 2021 remained unchanged at around 82%. Likewise, the largest net change occurred in the areas of WB, AL, and UA, at 51, 22, and 21%, respectively. Meanwhile, forest cover reported a loss of 4% (165.09 km2 ) of the total area in the analyzed period (2017–2021). The application of GEE allowed for an evaluation of the changes in forest cover and land use in the dry forest, and from this, it provided important information for the sustainable management of this ecosystemÍtem First draft genome assembly of the Peruvian creole cattle breed (Bos taurus) and its comparative genomics among the Bovinae subfamily(MDPI, 2022-08-18) Estrada Cañari, Richard; Corredor Arizapana, Flor Anita; Figueroa Venegas, Deyanira Antonella; Salazar Coronel, Wilian; Quilcate Pairazamán, Carlos Enrique; Vásquez Pérez, Héctor Vladimir; Maicelo Quintana, Jorge Luis; Gonzales, Jhony; Arbizu Berrocal, Carlos IrvinThe Peruvian creole cattle (PCC) is a neglected breed, and is an essential livestock resource in the Andean region of Peru. To develop a modern breeding program and conservation strategies for the PCC, a better understanding of the genetics of this breed is needed. We sequenced the whole genome of the PCC using a paired-end 150 strategy on the Illumina HiSeq 2500 platform, obtaining 320 GB of sequencing data. The obtained genome size of the PCC was 2.77 Gb with a contig N50 of 108Mb and 92.59% complete BUSCOs. Also, we identified 40.22% of repetitive DNA of the genome assembly, of which retroelements occupy 32.39% of the total genome. A total of 19,803 protein-coding genes were annotated in the PCC genome. We downloaded proteomes and genomes of the Bovinae subfamily, and conducted a comparative analysis with our draft genome. Phylogenomic analysis showed that PCC is related to Bos indicus. Also, we identified 7,746 family genes shared among the Bovinae subfamily. This first PCC genome is expected to contribute to a better understanding of its genetics to adapt to the tough conditions of the Andean ecosystem, and evolution.Ítem Modeling the current and future habitat suitability of Neltuma pallida in the dry forest of northern Peru under climate change scenarios to 2100(John Wiley & Sons Inc., 2024-08-27) Barboza Castillo, Elgar; Bravo Morales, Nino; Cotrina Sanchez, Alexander; Salazar Coronel, Wilian; Gálvez Paucar, David; Gonzales, Jhony; Saravia Navarro, David; Valqui Valqui, Lamberto; Cárdenas Rengifo, Gloria Patricia; Ocaña Reyes, Jimmy Alcides; Cruz Luis, Juancarlos; Arbizu Berrocal, Carlos IrvinThe development of anthropic activities and climate change effects impact worldwide species' ecosystems and habitats. Habitats' adequate prediction can be an important tool to assess current and future trends. In addition, it allows strategies development for their conservation. The Neltuma pallida of the forest region in northern Peru, although very significant, has experienced a decline in recent years. The objective of this research is to evaluate the current and future distribution and conservation status of N. pallida in the Peruvian dry forest under climate change (Location: Republic of Peru). A total of 132 forest presence records and 10 variables (bioclimatic, topographic, and soil) were processed and selected to obtain the current and future distribution for 2100, using Google Earth Engine (GEE), RStudio, and MaxEnt. The area under the curve values fell within the range of 0.93–0.95, demonstrating a strong predictive capability for both present and future potential habitats. The findings indicated that the likely range of habitats for N. pallida was shaped by factors such as the average temperature of wettest quarter, maximum temperature of warmest month, elevation, rainfall, and precipitation of driest month. The main suitable areas were in the central regions of the geographical departments of Tumbes, Piura, and Lambayeque, as well as in the northern part of La Libertad. It is critical to determine the habitat suitability of plant species for conservation managers since this information stimulates the development of policies that favor sustainable use programs. In addition, these results can contribute significantly to identify new areas for designing strategies for populations conserving and recovering with an ecological restoration approach.Ítem Reference-Guided Draft Genome Assembly, Annotation and SSR Mining Data of the Peruvian Creole Cattle (Bos taurus)(MDPI, 2022-11-09) Estrada Cañari, Richard; Corredor Arizapana, Flor Anita; Figueroa, Deyanira; Salazar Coronel, Wilian; Quilcate Pairazamán, Carlos Enrique; Vásquez Pérez, Héctor Vladimir; Maicelo Quintana, Jorge Luis; Gonzales, Jhony; Arbizu Berrocal, Carlos IrvinThe Peruvian creole cattle (PCC) is a neglected breed and an essential livestock resource in the Andean region of Peru. To develop a modern breeding program and conservation strategies for the PCC, a better understanding of the genetics of this breed is needed. We sequenced the whole genome of the PCC using a de novo assembly approach with a paired-end 150 strategy on the Illumina HiSeq 2500 platform, obtaining 320 GB of sequencing data. A reference scaffolding was used to improve the draft genome. The obtained genome size of the PCC was 2.81 Gb with a contig N50 of 108 Mb and 92.59% complete BUSCOs. This genome size is similar to the genome references of Bos taurus and B. indicus. In addition, we identified 40.22% of repetitive DNA of the genome assembly, of which retroelements occupy 32.39% of the total genome. A total of 19,803 protein-coding genes were annotated in the PCC genome. For SSR data mining, we detected similar statistics in comparison with other breeds. The PCC genome will contribute to a better understanding of the genetics of this species and its adaptation to tough conditions in the Andean ecosystem.Ítem The Complete Mitochondrial Genome of a Neglected Breed, the Peruvian Creole Cattle (Bos taurus), and Its Phylogenetic Analysis(MDPI, 2022-06-06) Arbizu Berrocal, Carlos Irvin; Ferro Mauricio, Rubén Darío; Chávez Galarza, Julio César; Vásquez Pérez, Héctor Vladimir; Maicelo Quintana, Jorge Luis; Poemape Tuesta, Carlos Augusto; Gonzales, Jhony; Quilcate Pairazamán, Carlos Enrique; Corredor Arizapana, Flor AnitaCattle spread throughout the American continent during the colonization years, originating creole breeds that adapted to a wide range of climate conditions. The population of creole cattle in Peru is decreasing mainly due to the introduction of more productive breeds in recent years. During the last 15 years, there has been significant progress in cattle genomics. However, little is known about the genetics of the Peruvian creole cattle (PCC) despite its importance to (i) improving productivity in the Andean region, (ii) agricultural labor, and (iii) cultural traditions. In addition, the origin and phylogenetic relationship of the PCC are still unclear. In order to promote the conservation of the PCC, we sequenced the mitochondrial genome of a creole bull, which also possessed exceptional fighting skills and was employed for agricultural tasks, from the highlands of Arequipa for the first time. The total mitochondrial genome sequence is 16,339 bp in length with the base composition of 31.43% A, 28.64% T, 26.81% C, and 13.12% G. It contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. Among the 37 genes, 28 were positioned on the H-strand and 9 were positioned on the L-strand. The most frequently used codons were CUA (leucine), AUA (isoleucine), AUU (isoleucine), AUC (isoleucine), and ACA (threonine). Maximum likelihood reconstruction using complete mitochondrial genome sequences showed that the PCC is related to native African breeds. The annotated mitochondrial genome of PCC will serve as an important genetic data set for further breeding work and conservation strategies.