Examinando por Autor "Galindo, Walter"

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Changes in gut microbiota in peruvian cattle genetic nucleus by breed and correlations with beef quality
(MDPI, 2024-11-29) Quilcate Pairazamán, Carlos Enrique; Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Rojas Cruz, Diorman; Mamani Chullo, Rolando; Hañari Quispe, Renán Dilton; Aliaga, Mery; Galindo, Walter; Vásquez, Héctor V.; Maicelo, Jorge L.; Arbizu, Carlos I.
This study evaluated the gut microbiota and meat quality traits in 11 healthy female cattle from the Huaral region of Peru, including 5 Angus, 3 Braunvieh, and 3 F1 Simmental × Braunvieh. All cattle were 18 months old and maintained on a consistent lifelong diet. Meat quality traits, including loin area, fat thickness, muscle depth, and marbling, were assessed in vivo using ultrasonography. Fecal samples were collected for microbiota analysis, and DNA was extracted for 16S and 18S rRNA sequencing to characterize bacterial, fungal, and protist communities. Significant correlations were observed between microbial genera and meat traits: Christensenellaceae R-7 and Alistipes were positively associated with marbling and muscle area, while Rikenellaceae RC9 showed a negative correlation with fat thickness. Among fungi, Candida positively correlated with marbling, while Trichosporon was negatively associated with muscle depth. For protists, Entodinium negatively correlated with fat thickness and marbling. Alpha diversity varied by breed, with Angus showing greater bacterial diversity, and beta diversity analyses indicated a strong breed influence on microbial composition. These findings suggest that microbial composition, shaped by breed and dietary consistency, could serve as an indicator of meat quality, offering insights into gut microbiota’s role in optimizing cattle production.
Effects of age in fecal microbiota and correlations with blood parameters in genetic nucleus of cattle
(MDPI, 2024-06-29) Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Figueroa Venegas, Deyanira Antonella; Coila Añasco, Pedro Ubaldo; Hañari Quispe, Renán Dilton; Aliaga, Mery; Galindo, Walter; Alvarado Chuqui, Wigoberto; Casanova Nuñez Melgar, David Pavel; Quilcate Pairazamán, Carlos Enrique
This study aimed to determine the impact of age on the fecal microbiota in the genetic nucleus of cattle, with a focus on microbial richness, composition, functional diversity, and correlations with blood parameters. Fecal and blood samples from 21 cattle were analyzed using 16S rRNA gene sequencing. Older cattle exhibited greater bacterial diversity and abundance, with significant changes in alpha diversity indices (p < 0.05). Beta diversity analysis revealed significant variations in microbial composition between age groups and the interaction of age and sex (p < 0.05). Correlations between alpha diversity, community composition, and hematological values highlighted the influence of microbiota on bovine health. Beneficial butyrate-producing bacteria, such as Ruminococcaceae, were more abundant in older cattle, suggesting a role in gut health. Functional diversity analysis indicated that younger cattle had significantly more abundant metabolic pathways in fermentation and anaerobic chemoheterotrophy. These findings suggest management strategies including tailored probiotic therapies, dietary adjustments, and targeted health monitoring to enhance livestock health and performance. Further research should include comprehensive metabolic analyses to better correlate microbiota changes with age-related variations, enhancing understanding of the complex interactions between microbiota, age, and reproductive status.
Effects of Diet and Altitude on the Microbiota of the First Compartment of the Stomach in Peruvian Alpacas in Highland Puna Regions and Their Correlations with Blood Parameters
(MDPI, 2026-01-08) Flores Huarco, Nils H.; Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Coila, Pedro; Sanchez, Diana; Maicelo, Jorge L.; Alvarado, Wigoberto C.; Quilcate Pairazaman, Carlos Enrique; Aliaga, Mery; Galindo, Walter; Saavedra Ramírez, Jorge; Apaza, Henry; Arbizu, Carlos I.
This study explores the intestinal microbiota of eight 18-month-old male alpacas from two distinct high-altitude regions in Peru: the Wet Puna (4200 m above sea level) and the Dry Puna (4900 m above sea level). Using 16S rRNA and 18S rRNA metabarcoding, microbial communities of bacteria, archaea, fungi, and protists were analyzed from the first compartment of the stomach (C1) to investigate the diversity, taxonomic composition, and correlations with hematological parameters. Significant differences in microbial diversity and composition were observed between regions, driven by dietary and environmental factors. The Wet Puna exhibited greater alpha diversity in bacterial and fungal communities, while beta diversity highlighted distinct microbial compositions. Key taxa, such as Prevotella ruminicola and Acetitomaculum, were associated with energy metabolism and host adaptation, whereas methanogenic archaea (Methanobrevibacter, Methanosphaera) dominated in the Dry Puna, reflecting adaptations to arid conditions. Correlations between microbial taxa and hematological variables, such as Acetitomaculum with red blood cell count and Eremoplastron with neutrophil percentage, emphasize the complex interplay between microbiota and host physiology. These findings contribute to understanding microbial adaptations in high-altitude livestock and provide practical insights for enhancing alpaca management and conservation strategies through tailored nutritional approaches and sustainable grazing practices.