Examinando por Autor "Fernandez Huaytalla, Elizabeth"

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First report of cassava common mosaic disease and Cassava Common Mosaic Virus infecting cassava (Manihot esculenta) in Peru
(American Phytopathological Society, 2017-04-03) Fernandez Huaytalla, Elizabeth; Espinoza, Ivonne; Lozano, Ivan; Bolaños, Carmen; Carvajal Yepes, Monica; Cuellar, Wilmer
Cassava common mosaic disease (CCMD) can cause root yield losses of approximately 30% (Venturini et al. 2016) in cassava (Manihot esculenta Crantz) and it has already been reported in Brazil, Colombia, Paraguay, and Argentina (Calvert et al. 2012; Di Feo et al. 2015). Most of Peru’s cassava production is in the eastern side of the country (the rainforest region) and is mainly used for direct human consumption. Cultivated area in these regions is approximately 48.1 thousand hectares (MINAGRI 2015). CCMD is caused by Cassava common mosaic virus (CsCMV; Calvert et al. 1996), a mechanically transmitted potexvirus that can be disseminated via infected stem cuttings used for cassava propagation. Given the presence of the disease in neighboring countries, a field survey for virus diseases in cassava was organized during June 2016 in the province of Huaral, in the central coast of Peru, where typical leaf mosaic and leaf deformation symptoms associated to CCMD were observed in local cassava varieties. To verify the presence of CsCMV and CCMD in Peru, the youngest leaves of four plants showing virus-like symptoms and four plants not showing symptoms were collected from one of the affected fields and dried in silica gel for analysis. Double antibody sandwich (DAS)-ELISA tests using a polyclonal antiserum readily detected CsCMV in all symptomatic samples (Nolt et al. 1991). In addition, mechanical transmissions to the experimental host Nicotiana benthamiana induced typical systemic leaf mosaic. RT-PCR tests targeting the replicase region of CsCMV were carried out using primers CsCMV-3269-F: 5′-GAGGCTCTTCTCTGGGAAAC-3′ and CsCMV-3896-R: 5′-CTTGAGTCCAGTTTGATGTC-3′, designed using an alignment of CsCMV-related sequences available in GenBank. An expected PCR fragment of 627 bp was obtained only in samples showing symptoms of CCMD. RT-PCR tests for other cassava-infecting viruses reported in the Americas (Carvajal-Yepes et al. 2014) were negative in these samples. PCR products from two independent CsCMV-positive samples were sent for direct Sanger-sequencing (Macrogen, Korea). CsCMV sequence isolates from Peru (GenBank accession nos. KX964625 and KX964626) show a nucleotide identity of 88 to 93%, and an amino acid sequence identity of 99% with other CsCMV sequences available in GenBank, and phylogenetic analysis clustered Peruvian isolates with CsCMV sequences reported in cassava. These results stress the need to implement surveillance activities and quick diagnostic protocols, as the inadvertent propagation and accumulation of virus infections could cause an increasingly negative effect on cassava and other vegetatively propagated crops.
Morpho-Physiological response of four native accessions of Phaseolus vulgaris L. subjected to water stress under greenhouse conditions in northeastern Peru
(MDPI, 2024-09-06) Santillan Huaman, Anlly; Tejada Alvarado, José Jesús; García, Ligia; Vásquez García, Jheiner; Fernandez Huaytalla, Elizabeth; Vilca Valqui, Nuri Carito; Oliva Cruz, Manuel
Phaseolus vulgaris L. is a legume of high nutraceutical value, widely cultivated and consumed. However, common bean production faces challenges such as water stress that severely affects its growth and yield. This study evaluated the morphological and physiological response of four native P. vulgaris accessions subjected to different irrigation treatments under greenhouse conditions. A completely randomized design with factorial arrangement was used, evaluating three irrigation frequencies (100%, 50%, 25%) in combination with four accessions (PER1003541, PER1003542, PER1003543, PER1003544). The results showed that with the 25% irrigation treatment, PER1003544 showed a 54.62% decrease in leaf area, while PER1003542 and PER1003543 experienced reductions of 56.56% and 59.24%, respectively. In addition, accession PER1003544 reported a smaller reduction in the number of flowers and pods, with decreases of 40.21% and 29.9%, in contrast to PER1003543, which showed decreases of 60.66% and 52.63%, respectively. Accessions PER1003541 and PER1003544 also recorded the lowest reductions in dry biomass, with 31.85%and 35.41%, respectively. Regarding yield, PER1003544 and PER1003541 experienced reductions of 59.01% and 69.79%, respectively, unlike PER1003543, which showed a 90% decrease. In relation to stomatal density, PER1003541 recorded a reduction of 28.28%, while PER1003544 had a decrease of 37.10%, and PER1003543 experienced a reduction of 47.05%; chlorophyll content showed a similar trend. Finally, PER1003544 maintained a relatively stable stomatal index, with a reduction of 29.01%, compared to PER1003543, which reduced by 60.99%. In conclusion, accession PER1003544 stands out as a promising variety for breeding programs focused on water stress tolerance, contributing to food security and agricultural sustainability in areas affected by limited water availability. However, PER1003541 would be a suitable additional option, offering farmers flexibility in their crop selection according to the specific conditions of their environment.
The complete chloroplast genome of a centennial olive tree (Olea europaea, Oleaceae) from the southern Peruvian coast
(F1000Research, 2024-12-04) Saldaña, Carla L.; Chura Llanos, Luz S.; Estrada Cañari, Richard; Fernandez Huaytalla, Elizabeth; Zirena Vilca, Franz; Guerrero Abad, Juan Carlos; Amasifuen Guerra, Carlos Alberto; Gutierrez Reynoso, Dina Lida; Injante Silva, Pedro Hugo; Arbizu, Carlos I
Olive (Olea europaea Linaeus 1753) is one of the valuable fruit trees and very important edible oil plant in the world. The chloroplast (cp) genome of an olive tree (Olea europaea) from the southern Peruvian arid coast was obtained for the first time. Genomic DNA of high quality was used to generate librarieswith Illumina Hiseq paired-end methods. The cp genome is 155,886 pb in length and contains a large single-copy region (LSC) of 86,610 pb and a small single copy region (SSC) of 17,790 pb separated by two inverted repeat (IR) regions (25,741bp). The cp genome of olive contains 124 genes that consists of 80 protein-coding genes, 36 tRNA, eight rRNA. Phylogenetic analysis showed this olive tree is sister to O. europaea subsp. maroccana ( Oleeae tribe). This study presents the first overview of the chloroplast genome organization and phylogenetics of O. europaea, offering valuable insights for genetic and evolutionary research in the genus Olea.
Uso de maracuyá (Passiflora edulis f. flavicarpa) como portainjerto para la propagación de especies de Passiflora supersect. Tacsonia colectadas en Perú
(Universidad Nacional Agraria La Molina, Facultad de Ciencias Forestales, 2024-02-05) Chávez Corcuera, Gonzalo; Torres Chacón, Rosmery; Fernandez Huaytalla, Elizabeth; Elías Da Silva, Rosa Angelica; Gutiérrez Reynoso, Dina Lida
Las especies de Passiflora supersect. Tacsonia (Juss.) Feuillet & J.M. MacDougal son plantas adaptadas a los ecosistemas andinos que producen frutos comestibles. Estos son cultivados y comercializados a pequeña escala para su consumo como fruta fresca y para la elaboración de postres y bebidas. Ante las fuertes perturbaciones antrópicas y ambientales que sufre el hábitat natural de las especies de Passiflora L., la extinción parcial o total de ellas es una realidad latente. Por ello, con el fin de conservar estos recursos en Bancos de Germoplasma, es necesario el desarrollo de métodos óptimos para la propagación de las distintas especies. Con la intención de conservar ex situ, en la Colección de Passiflora del Banco de Germoplasma del Instituto Nacional de Innovación Agraria (INIA) a las especies de Passiflora supersect. Tacsonia, se verificó la viabilidad del uso de Passiflora edulis f. flavicarpa O. Deg. como portainjerto para la propagación asexual de seis taxa seleccionados. Passiflora tripartita var. mollissima (Kunth) Holm-Niels. & P. Jørg., P. cumbalensis (H. Karst.) Harms, P. pinnatistipula Cav., P. tarminiana Coppens & V.E. Barney, P. peduncularis Cav. y P. trifoliata Cav. var. trifoliata fueron injertados en P. edulis f. flavicarpa obteniendo un éxito de prendimiento y aclimatación óptima para todos los taxa seleccionados. Adicionalmente, todos los taxa con excepción de P. trifoliata var. trifoliata produjeron flores y frutos. Finalmente, este es el primer registro de propagación y cultivo exitoso para P. trifoliata y P. peduncularis, ambas especies endémicas de Perú, en un hábitat cercano al nivel del mar.