Examinando por Autor "Corazon Guivin, Mike Anderson"

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A pathogen complex between the root knot nematode Meloidogyne incognita and Fusarium verticillioides results in extreme mortality of the inka nut (Plukenetia volubilis)
(Julius Kühn-Institut., 2021-10-12) Guerrero Abad, Juan Carlos; Padilla Domínguez, Ammer; Torres Flores, Elías; López Rodríguez, Carlos; Guerrero Abad, Roger Arbildo; Coyne, Danny; Oehl, Fritz; Corazon Guivin, Mike Anderson
The combined infection of the root knot nematode Meloidogyne incognita and two Fusarium species led to high plant mortality of inka nut (Plukenetia volubilis) seedlings in pots after 80 days growth in two independent inoculation experiments. Inoculation of M. incognita juveniles and conidia of F. solani or F. verticillioides, simultaneously, increased plant mortality by 25-30%, compared with M. incognita alone (5-10% mortality). When inoculated with F. verticillioides at 20 days post nematode inoculation, plant mortality increased to 55%. Either of the Fusarium spp. alone caused some plant mortality, but less than when combined with M. incognita. The synergistic interaction of M. incognita and especially F. verticillioides demonstrates a lethal outcome for inka nuts. It appears that M. incognita creates favorable conditions that lead to enhanced effect and damage by the Fusarium species, especially F. verticillioides, leading to devastating levels of plant death.
Acaulospora aspera, a new fungal species in the Glomeromycetes from rhizosphere soils of the inka nut (Plukenetia volubilis L.) in Peru
(Julius Kühn-Institut, 2019-10-09) Corazon Guivin, Mike Anderson; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Vallejos Tapullima, Adela; Carballar Hernández, Santos; Alves da Silva, Gladstone; Oehl, Fritz
A new fungal species of the Glomeromycetes, Acaulospora aspera, was isolated from the rhizosphere of the inka nut (Plukenetia volubilis) in San Martín State of Peru (Western Amazonia) and propagated in bait cultures on Sorghum spp., Brachiaria brizantha, Medicago sativa and P. volubilis as host plants. The fungus forms brownish yellow to yellow brown spores, (120-)135-195 × (120-)130 187 μm in diameter. The surface of the structural spore wall layer is crowded with small depressions, 0.4-0.7 μm in diameter, up to 0.8 μm deep, and only 1.1-1.8 apart, giving the spore surface a rough, washboardlike appearance, especially when the outermost, evanescent wall layer has disappeared. Phylogenetically, the new species is close to A. spinosissima, A. excavata and to other morphologically more similar species such as A. spinosa and A. tuberculata, which form spiny or tuberculate projections on the outermost, semi-persistent spore wall layer, or A. herrerae, A. kentinensis, A. scrobiculata and A. minuta, which on the structural spore wall layer all have more pronunced pits than A. aspera. In this study, also the name of A. spinosissima was validated, as it had been preliminary declared invalid because of a typing error in the diagnosis section of its original description.
Acaulospora flava, a new arbuscular mycorrhizal fungus from Coffea arabica and Plukenetia volubilis plantations at the sources of the Amazon river in Peru
(Julius Kühn-Institut, 2021-08-06) Corazon Guivin, Mike Anderson; Vallejos Tapullima, Adela; De la Sota Ricaldi, Ana María; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Santos, Viviane Monique; Da Silva, Glandstone Alves; Oehl, Fritz
A new arbuscular mycorrhizal fungus, Acaulospora flava, was found in coffee (Coffea arabica) and inka nut (Plukenetia volubilis) plantations in the Amazonia region of San Martín State in Peru. The fungus was propagated in bait cultures on Sorghum vulgare, Brachiaria brizantha and Medicago sativa as host plants. It differentiates typical acaulosporoid spores laterally on sporiferous saccule necks. The spores are light yellow, bright yellow to yellow brown, (95-)105-160 × (95-)100-150 μm in diameter and have smooth spore surfaces. Phylogenetically, A. flava clusters in a well-separated clade, nearest to A. kentinensis, followed by A. herrerae, A. spinosissima, A. excavata and A. aspera, of which remarkably A. spinosissima, A. excavata and A. aspera had also been found in inka nut plantations of San Martín State during the last years. Here, we report also A. herrerae and A. fragilissima as fungal symbionts within the rhizosphere of coffee and the inka nut. The later two fungi had so far been recorded by concomitant morphological and molecular analyses only from tropical islands, A. herrerae from Cuba in the Golf of Mexico and A. fragilissima from New Caledonia in the southwest Pacific Ocean close to Australia. In this study, the ITS region of A. herrerae was analyzed for the first time and deposited in the public databases. In total, we already recovered fourteen Acaulospora species from coffee and inka nut plantations in San Martín State of Peru, suggesting that Acaulospora species are frequent and beneficial symbionts in coffee and inka nut roots in San Martín State of Peru.
Arbuscular mycorrhizal fungi associated with Myrciaria dubia in the Amazonia Region, Peru
(Hindawi, 2024-01-30) Pineda Lázaro, Alexandra Jherina; Vallejos Tapullima, Adelaide S.; Hernández Amasifuen, Angel David; Carballar Hernández, Santos; Imán Correa, Sixto Alfredo; Carvajal Vallejos, Fernando Marcelo; Ríos Ramírez, Orlando; Corazon Guivin, Mike Anderson
Myrciaria dubia (Kunth) McVaugh (camu-camu) is a shrub native to the Amazon region that produces fruits with a high content of vitamin C and various bioactive compounds, making it a functional food with antioxidant, anti-inflammatory, and antimicrobial properties. However, it is unknown which microorganisms are associated with its root system and can influence its growth and productivity. Arbuscular mycorrhizal fungi (AMF) are associated with most plants and are essential for their establishment, survival, and productivity since they facilitate their nutrition, increase water absorption, and improve soil structure. Although the AMF association is already known in some species of Myrciaria, no report is available on its association in M. dubia. This study presents, for the first time, the symbiotic association between AMF and M. dubia from the INIA San Roque experimental station located in the Amazon region, Peru. For the morphological and molecular analyses of the AMF, samples of rhizospheric soil and roots from two native accessions of the National Germplasm Bank of M. dubia were collected. Eighteen AMF morphospecies were identified in rhizospheric soil, belonging to nine genera Acaulospora, Ambispora, Entrophospora, Diversispora, Gigaspora, Glomus, Paraglomus, Funneliformis, and Sclerocystis, being the first one the most frequent. The roots of M. dubia showed high colonization by AMF (mean =91%), and characteristic structures of arbuscular mycorrhizae, such as vesicles, hyphae, and arbuscules, could be observed. Likewise, the molecular analysis detected the presence of genetic material (rDNA) corresponding to AMF in the roots of both accessions. Our results evidenced the symbiotic association between AMF and M. dubia, which encourages further investigation of the functional potential of these microorganisms in this economically crucial agricultural plant in Peru.
Caracterización genética de accesiones Sanmartinenses del Banco Nacional de Germplasma de Sacha Inchi Plukenetia volubilis L. (E.E. El Porvenir-INIA)
(Instituto de Investigaciones de la Amazonía Peruana - IIAP, 2009-12-31) Corazon Guivin, Mike Anderson; Castro Ruiz, Diana; Chota Macuyama, Werner; Rodríguez, Ángel; Cachique, Danter; Manco Céspedes, Emma Imelda; Del Castillo, Dennis; Renno, Jean François; García Dávila, Carmen
[ES] El presente estudio analiza 12 accesiones sanmartinenses de Sacha Inchi Plukenetia volubilis del Banco Nacional de Germoplasma del INIA, utilizando la técnica molecular DALP. Para lo cual se colectaron muestras (Hojas) de un total de 24 plantas (dos por cada accesión), que fueron evaluadas utilizando los marcadores informativos (DALP221 y DALP233). El análisis de los perfiles genéticos entre las accesiones, mostró una alta diversidad con un total de 23 genotipos diferentes, observando que casi todas las accesiones analizadas presentaron dos genotipos, a excepción de los individuos Cub-02 y Ba.01-14 que compartieron un mismo genotipo. La planta SL-I-19 (accesión SL-I) mostró una fuerte divergencia genética, lo que sugiere la presencia de genotipos adicionales. Los métodos moleculares pueden hacer importantes contribuciones en la cuantificación de la diversidad genética de Sacha Inchi y otras especies de plantas en el Perú del Banco Nacional de Germoplasma. Este conocimiento es vital en la conservación y mejora genética de la especie.--- [EN] The present study examines 12 accessions of Sacha Inchi (Plukenetia volubilis) from San Martín of Peruvian National Germplasm Bank (INIA), using molecular techniques (DALP). Leaves were collected from a total of 24 individuals (two per accession), and evaluated using the informative markers DALP221 and DALP233. Genetic analysis showed high diversity between accessions, with a total of 23 different genotypes represented. In all but one case, the individuals of a given accession differed in genotype; only Cub-02 and Ba.01-14 shared a single genotype. Individual SL-I-19 showed strong genetic divergence, suggesting the presence of additional genotypes. Molecular methods can make important contributions in quantifying the genetic diversity of Sacha Inchi and other plant species in the Peruvian National Germplasm Bank. This knowledge is vital in the conservation and genetic improvement of the species.
Fungal Systematics and Evolution: FUSE 5
(Verlag Ferdinand Berger und Sohne GmbH, 2019-12-23) Corazon Guivin, Mike Anderson; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Vallejos Tapullima, Adela; Carballar Hernández, Santos; Alves da Silva, Gladstone; Oehl, Fritz
Thirteen new species are formally described: Cortinarius brunneocarpus from Pakistan, C. lilacinoarmillatus from India, Curvularia khuzestanica on Atriplex lentiformis from Iran, Gloeocantharellus neoechinosporus from China, Laboulbenia bernaliana on species of Apenes, Apristus, and Philophuga (Coleoptera, Carabidae) from Nicaragua and Panama, L. oioveliicola on Oiovelia machadoi (Hemiptera, Veliidae) from Brazil, L. termiticola on Macrotermes subhyalinus (Blattodea, Termitidae) from the DR Congo, Pluteus cutefractus from Slovenia, Rhizoglomus variabile from Peru, Russula phloginea from China, Stagonosporopsis flacciduvarum on Vitis vinifera from Italy, Strobilomyces huangshanensis from China, Uromyces klotzschianus on Rumex dentatus subsp. klotzschianus from Pakistan. The following new records are reported: Alternaria calendulae on Calendula officinalis from India; A. tenuissima on apple and quince fruits from Iran; Candelariella oleaginescens from Turkey; Didymella americana and D. calidophila on Vitis vinifera from Italy; Lasiodiplodia theobromae causing tip blight of Dianella tasmanica ‘variegata’ from India; Marasmiellus subpruinosus from Madeira, Portugal, new for Macaronesia and Africa; Mycena albidolilacea, M. tenuispinosa, and M. xantholeuca from Russia; Neonectria neomacrospora on Madhuca longifolia from India; Nothophoma quercina on Vitis vinifera from Italy; Plagiosphaera immersa on Urtica dioica from Austria; Rinodina sicula from Turkey; Sphaerosporium lignatile from Wisconsin, USA; and Verrucaria murina from Turkey. Multi-locus analysis of ITS, LSU, rpb1, tef1 sequences revealed that P. immersa, commonly classified within Gnomoniaceae (Diaporthales) or as Sordariomycetes incertae sedis, belongs to Magnaporthaceae (Magnaporthales). Analysis of a six-locus Ascomycota-wide dataset including SSU and LSU sequences of S. lignatile revealed that this species, currently in Ascomycota incertae sedis, belongs to Pyronemataceae (Pezizomycetes, Pezizales).
Funneliglomus, gen. nov., and Funneliglomus sanmartinensis, a new arbuscular mycorrhizal fungus from the Amazonia region in Peru
(Verlag Ferdinand Berger & Söhne, 2019-03-11) Corazon Guivin, Mike Anderson; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Vallejos Tapullima, Adela; Carballar Hernández, Santos; Alves da Silva, Gladstone; Oehl, Fritz
A new arbuscular mycorrhizal fungus was found in agricultural field sites in the Amazonia lowlands of the Department San Martin, Perú. It was found up to 858 m above sea level in two plantations of the inka nut (also called ‚sacha inchi', Plukenetia volubilis), which was grown in mixed cultures together with Musa sp., Zea mays, Phaseolus vulgaris and Carica papaya. The fungus was propagated in bait cultures in a greenhouse on Sorghum sp., Brachiaria sp., Medicago sativa and Plukenetia volubilis as host plants. The fungus differentiates orange brown to dark orange brown, triple-layered spores, 93-151× 90-148 µm in diameter, terminally on funnel-shaped to rarely cylindrical or slightly inflating hyphae, and a strong, straight to slightly recurved septum that closes the spore pore at the spore base. Phylogenetically, the new fungus represents clearly a new genus in a separated clade, near to Funneliformis and Septoglomus. It can easily be distinguished from all other species of these genera by the characteristic ornamentation of the spore surface, which consists of multiple large, irregular pits. The fungus is here described under the epithet Funneliglomus sanmartinensis, serving as type species of the new genus Funneliglomus.
Global studies of cadmium in relation to Theobroma cacao: A bibliometric analysis from Scopus (1996 -2020)
(Universidad Nacional de Trujillo, 2021-12-15) García, Ligia; Angulo Castro, Fabio; Hernández Amasifuen, Angel David; Corazon Guivin, Mike Anderson; Alburquerque Vásquez, Javier; Guerrero Abad, Juan Carlos; Arellanos, Erick; Veneros, Jaris; Rojas Briceño, Nilton B.; Chavez Quintana, Segundo G.; Oliva Cruz, Manuel
En el año 2014, la Unión Europea impuso estándares máximos de tolerancia en cadmio, para la importación de productos a base de cacao, causando preocupación en los países. Se analizó estudios globales referidos a investigaciones en Theobroma cacao, relacionados con la actividad del cadmio en la atmósfera. Se utilizaron análisis bibliométricos en los programas R y VOSviewer, para examinar 64 documentos publicados en la base de datos Scopus según palabras clave. Se identificaron 811 palabras clave en coocurrencias de términos, 5 grupos temáticos en acoplamiento bibliográfico, 20 instituciones como afiliaciones más importantes, 20 países de procedencia de autores corresponsales, 112 instituciones en red de coautoría de los cuales 5 están en documentos primarios, y dos grupos en similaridad temática en co-citación de documentos. Estados Unidos lidera la producción científica con 11 documentos, seguido de Colombia (8) y Ecuador (7). En 1996 se registró el primer artículo científico para la red, con incrementos de hasta 11 publicaciones al 2020. En conclusión, se evidencia la necesidad de fortalecer y crear más redes de investigaciones entre países, instituciones, autores y coautores. Se espera que los resultados permitan desentrañar de manera integral la trayectoria de investigaciones cadmio-cacao, al tiempo que arrojen nuevas investigaciones prospectivas.
Massive production of Hemileia vastatrix uredospores for infection of Coffea arabica seedlings
(Universidad Nacional de Trujillo, 2023-07-10) Hernández Amasifuen, Angel David; Rivadeneyra Chisquipama, Lenin; Padilla Dominguez, Amner; Paredes Espinosa, Richard; Guerrero Abad, Juan Carlos; Torres Flores, Elías; Peláez Rivera, Jorge Luis; Carvajal Vallejos, Fernando Marcelo; Gutiérrez Reynoso, Dina Lida; Corazon Guivin, Mike Anderson
Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the multiplication process of H. vastatrix uredospores, using controlled conditions (23±1 °C, ≈ 80% relative humidity and photoperiod of 16 hours of light) under an innovative system, the first symptoms of chlorosis were observed on coffee leaves 20 days after inoculation. Our findings show a 100% incidence of inoculated coffee plants with an average of 54 mg of H. vastatrix uredospores per plant and a 1.38 x 10-1 mg/cm2 sporulation rate. Finally, here we report a protocol that allows mass multiplication of H. vastatrix uredospores under controlled conditions, that would be useful in any time for coffee leaf rust bioassays.
Nanoglomus plukenetiae, a new fungus from Peru, and a key to small-spored Glomeraceae species, including three new genera in the “Dominikia complex/clades”
(Springer Nature, 2019-11-22) Corazon Guivin, Mike Anderson; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Vallejos Tapullima, Adela; Carballar Hernández, Santos; Alves da Silva, Gladstone; Oehl, Fritz
A new arbuscular mycorrhizal fungus was found in an agricultural plantation of Plukenetia volubilis, the inka nut (also called “sacha inchi” or “inka peanut”) in the Amazonia region of San Martín State in Peru. In this site, the inka nut was grown in mixed cultures together with Zea mays and Phaseolus vulgaris. The fungus was propagated in bait and single-species cultures on Sorghum vulgare, Brachiaria brizantha, Medicago sativa, and Plukenetia volubilis as host plants. The fungus differentiates hyaline spores terminally or intercalary on cylindrical to slightly funnel-shaped hyphae, singly or in spore clusters with up to ca. 90 spores per cluster. The spores are bi-layered, (20–)25–36(–45) μm in diameter and show regularly a visible septum at the spore base, despite the small spore and tiny hyphae sizes. Phylogenetically, the new fungus represents a new genus in a separated clade, near to the already known Dominikia clades. It can be distinguished from other species by the small spore size, the characteristics of the spore wall layers, and the clearly visible septum at the spore base, which in Kamienskia and Microkamienskia species has rarely to never been reported, while Dominikia species usually have a higher variability of spore sizes and spore wall characteristics. The fungus is here described under the epithet Nanoglomus plukenetiae, serving as type species of the new genus Nanoglomus. The revision of the species and environmental sequences in the Dominikia clades, based on both morphological and molecular phylogenetic analyses, revealed at least two other new genera: Microdominikia gen. nov., based on D. litorea, and Orientoglomus gen. nov., based on D. emiratia. Finally, in the present study, a key for all small-spored species in the Glomeraceae is included comprising all known Dominikia, Kamienskia, Microdominikia, Microkamienskia, Nanoglomus, Orientoglomus spp., and all small-spored Rhizoglomus spp.
Paraglomus occidentale, a new arbuscular mycorrhizal fungus from the sources of the Amazon river in Peru, with a key to the Paraglomeromycetes species.
(Verlag Berger, 2020-02-06) Corazon Guivin, Mike Anderson; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Vallejos Tapullima, Adela; Ríos Ramírez, Orlando; Vallejos Torres, Geomar; De la Sota Ricaldi, Ana María; Santos, Viviane Monique; Alves da Silva, Gladstone; Oehl, Fritz
A new arbuscular mycorrhizal fungus, Paraglomus occidentale, was found in an agricultural plantation of the inka nut (Plukenetia volubilis) in the Amazonia region of San Martín State in Peru. The inka nut was grown in mixed cultures together with Zea mays and Phaseolus vulgaris. The fungus was propagated in bait and single species cultures on Sorghum vulgare, Brachiaria brizantha, Medicago sativa and P. volubilis as host plants. The fungus differentiates hyaline spores terminally on cylindrical to slightly funnel-shaped hyphae, singly in soils or rarely in roots. The hyaline spores have a triple layered outer wall and a bi- to triple-layered inner wall. They are (59)69–84(92) µm in diameter. The new fungus is distinguished from all other known Paraglomus spp. by spore wall structure including staining characteristics in Melzer’s reagent, which is yellow-grayish to grayish on the outermost and generally dark yellow on the second spore wall layer. Phylogenetically, the new fungus is recognized in a well-separated clade, near to P. laccatum and P. occultum. Finally, an identification key to the Paraglomeromycetes species is included comprising all known species of the genera Paraglomus, Innospora and Pervetustus.
Propagation of Theobroma cacao by rooted cuttings in mini-tunnels
(Hindawi, 2022-04-28) Vásquez Zamora, Lucía Melissa; Rengifo Del Aguila, Sofía; Guerrero Abad, Juan Carlos; Vallejos Torres, Geomar; Imán Correa, Sixto Alfredo; Torres Flores, Elías; Mesén Sequeira, Francisco; Corazon Guivin, Mike Anderson
Cacao is an economically important crop that is currently planted in Asia, Africa, and America. Cuttings is a technique of vegetative propagation suitable for the conservation of selected desirable characteristics in cacao trees. The objective of this study was to evaluate the rooting of cacao cuttings in mini-tunnels with different doses of indole-3-butyric acid (IBA) to obtain a simple and viable vegetative propagation protocol. The experiment was carried out under a completely randomized design (CRD). Cuttings 5 to 7 cm long were collected from the middle area of cacao tree crowns and treated with doses of 0, 1000, 2000, and 3000 ppm of IBA; then they were placed in Jiffy© pellets and set to root in plastic-covered mini-tunnels with fog irrigation. The results indicate that treatments with 0 and 1000 ppm of IBA produced the highest values in survival (100%, both treatments) and rooting percentage (87.7 and 90.0%, respectively) as well as number and length of roots (4.3 roots-4.21 cm in length and 4.5 roots-5.32 cm in length, respectively); likewise, cuttings treated with 0 ppm rooted after 24–40 days, followed by doses of 1000 ppm (24–46 days). All rooted cuttings without IBA (0 ppm) achieved 100% acclimatization in nursery. In general, the best results were obtained without IBA application (Control 0 ppm) in all the parameters evaluated, similar to those obtained with the application of 1000 ppm IBA; these results indicate the success and efficiency of the vegetative propagation protocol proposed in this study.