Examinando por Autor "Alvarado Chuqui, Wigoberto"

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Age-dependent changes in protist and fungal microbiota in a peruvian cattle genetic nucleus
(MDPI, 2024-08-14) Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Quilcate Pairazamán, Carlos Enrique; Dipaz Berrocal, Deisy Juana; Alejos Asencio, Carol Silvia; León Trinidad, Silvia Eliana; Alvarez García, Wuesley Yusmein; Rojas, Diorman; Alvarado Chuqui, Wigoberto; Maicelo Quintana, Jorge Luis; Arbizu, Carlos Irvin
In this research, the connection between age and microbial diversity in cattle was explored, revealing significant changes in both protist diversity and fungal microbiota composition with age. Using fecal samples from 21 Simmental cattle, microbial communities were analyzed through 18S rRNA gene sequencing. Results indicated significant differences in alpha protist diversity among the three age groups, while fungal composition varied notably with age and was linked to hematological parameters. Despite the stability of fungal alpha diversity, compositional changes suggest the gut as a stable niche for microbial colonization influenced by diet, clinical parameters, and microbial interactions. All cattle were maintained on a consistent diet, tailored to meet the specific nutritional needs of each age group. These findings emphasize the importance of understanding age-related microbial dynamics to enhance livestock management and animal health, contributing to broader ecological and biomedical research. This study was limited by the lack of comprehensive metabolic analyses correlating microbiota changes with specific age-related variations, indicating a need for further research in this área.
Agromorphological characterization of cacao (Theobroma cacao L.) accessions from the germplasm bank of the National Institute of Agrarian Innovation, Peru
(Elsevier, 2022-10-04) Vásquez García, Jheiner; Santos Peláez, Julio César; Malqui Ramos, Roiber; Vigo Mestanza, Carmen Natividad; Alvarado Chuqui, Wigoberto; Bobadilla Rivera, Leidy Gheraldine
Agromorphological characterization of cacao accessions in Peru is currently an important tool in the conservation and genetic improvement of cacao germplasm. The objective of this study was to carry out the morphological and agronomic characterization of 113 cacao accessions from the Huarangopampa germplasm bank. Tree, leaf, flower, fruit and seed descriptors were used. The data collected were processed by descriptive statistics using multivariate techniques. Five groups were formed according to similar characteristics. The accessions of group 1 are vigorous trees with an pod index of 19.27 pods/kg of seeds; the groups that presented better differential characteristics were group 2 with erect tree architecture, intermediate vigorousness, purple seed color and pod index of 20.07 pods/kg of seeds and group 3, which had the highest number of accessions with the lowest pod index of 18.77 pods/kg of seeds, besides being vigorous trees and having purple seeds. On the other hand, group 4 presented a particular characteristic of white seed color and high pod indexes with 22.11 pods/kg of seeds. Finally, group 5 accessions were characterized by intermediate tree architecture and vigor with an pod index of 21.3 pods/kg of seeds. The morphoagronomic characterization constitutes a first advance in the identification of cacaos with potential for genetic improvement and advances in the Peruvian chocolate industry.
Characterization of Coffea arabica L. parent plants and physicochemical properties of associated soils, Peru
(Cell Press, 2022-10-03) Alvarado Chuqui, Wigoberto; Bobadilla Rivera, Leidy Gheraldine; Valqui Valqui, Leandro; Silva Valqui, Gelver; Valqui Valqui, Lamberto; Vigo Mestanza, Carmen Natividad; Vásquez Pérez, Héctor Vladimir
It is important to carry out the morphological characterization of coffee parent plants and the physicochemical properties of the associated soils in the Amazon region, Peru, in order to achieve germplasm conservation. One hundred coffee mother plants were identified and located in five provinces of the region and evaluated according to morphological descriptors such as stipula shape, young leaf color, leaf shape, leaf apex shape, young shoot color, leaf color, fruit color, fruit shape, mature leaf color, and rust incidence percentage. In the plots where the parent plants were located, soil sampling was carried out to determine the physical and chemical properties. The varieties with the greatest presence in the five provinces were Típica and caturra roja, with the greatest number of specimens reported for the province of Bagua. The predominant stipule shape was triangular (91%), lanceolate leaf shape (60%) and red fruit color (90%). Bongará reported the lowest incidence of yellow rust, as well as the Mundo Novo Rojo variety. Soil pH ranged from acidic to neutral values, low electrical conductivity, high organic matter content, low phosphorus content, high potassium levels and medium cation exchange capacity. The predominant textural class was sandy loam. The physical and chemical characterization of the soils under study show favorable ranges to encourage the best development of coffee cultivation.
Complete mitogenome of “pumpo” (Bos taurus), a top bull from a Peruvian genetic nucleus, and its phylogenetic analysis
(MDPI, 2024-05-28) Estrada Cañari, Richard; Figueroa Venegas, Deyanira Antonella; Romero Avila, Yolanda; Alvarez García, Wuesley Yusmein; Rojas Cruz, Diorman; Alvarado Chuqui, Wigoberto; Maicelo Quintana, Jorge Luis; Quilcate Pairazamán, Carlos Enrique; Arbizu Berrocal, Carlos Irvin
The mitochondrial genome of Pumpo (Bos taurus), a prominent breed contributing to livestock farming, was sequenced using the Illumina HiSeq 2500 platform. Assembly and annotation of the mitochondrial genome were achieved through a multifaceted approach employing bioinformatics tools such as Trim Galore, SPAdes, and Geseq, followed by meticulous manual inspection. Additionally, analyses covering tRNA secondary structure and codon usage bias were conducted for comprehensive characterization. The 16,341 base pair mitochondrial genome comprises 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. Phylogenetic analysis places Pumpo within a clade predominantly composed of European cattle, reflecting its prevalence in Europe. This comprehensive study underscores the importance of mitochondrial genome analysis in understanding cattle evolution and highlights the potential of genetic improvement programs in livestock farming, thus contributing to enhanced livestock practices.
Draft genome sequence and SSR data mining of “pumpo” (Bos taurus), a top bull from a peruvian genetic nucleus
(MDPI, 2024-06-18) Estrada Cañari, Richard; Romero Avila, Yolanda; Figueroa Venegas, Deyanira Antonella; Quilcate Pairazaman, Carlos Enrique; Casanova Nuñez-Melgar, David Pavel; Vásquez Pérez, Hector Vladimir; Alvarado Chuqui, Wigoberto; Maicelo Quintana, Jorge Luis; Arbizu Berrocal, Carlos Irvin
Pumpo is a Simmental breed and an essential livestock resource in the nucleus genetic cattle of Peru. This study provides a draft genome sequence of a top bull using a de novo assembly approach on the Illumina Novaseq X platform, yielding 208 GB of raw sequencing data with 150 bp paired‐end reads. The final genome assembly resulted in a size of 2.06 Gb with an N50 contig length of 108 Mb and a completeness of 95.7% according to BUSCO analysis. A total of 973,925 simple sequence repeats (SSRs) were identified, with a predominance of mononucleotide repeats. The genome showed low heterozygosity (0.568%) and moderate repeatability (11.5%), aligning with other Bos taurus genomes. Reference‐guided scaffolding improved the assembly quality significantly, producing an N50 scaffold value of 108 Mb. The SSR analysis of the Pumpo genome identified 973,925 SSRs with a frequency of 2,808 SSRs per kilobase, predominantly mononucleotide repeats, and 85,453 found in compound formations. Obtaining knowledge of the genome of a breeding Simmental bull is essential to optimize breeding programs and improve productivity.
EEA Amazonas - Exposición de avance de metas: Agenda 2021
(Instituto Nacional de Innovación Agraria, 2021-10-14) Alvarado Chuqui, Wigoberto
Se presentan los avances relacionados a las siguientes actividades: Conservación y valoración de recursos genéticos, investigación y desarrollo tecnológico, vinculación tecnológica y fortalecimiento del SRIA, transferencia de tecnología y extensión agraria, aseguramiento de los servicios tecnológicos agrarios, fortalecimiento institucional (mantenimiento de la infraestructura) y ejecución presupuestal.
Effects of age in fecal microbiota and correlations with blood parameters in genetic nucleus of cattle
(MDPI, 2024-06-29) Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Figueroa Venegas, Deyanira Antonella; Coila Añasco, Pedro Ubaldo; Hañari Quispe, Renán Dilton; Aliaga, Mery; Galindo, Walter; Alvarado Chuqui, Wigoberto; Casanova Nuñez-Melgar, David Pavel; Quilcate Pairazamán, Carlos Enrique
This study aimed to determine the impact of age on the fecal microbiota in the genetic nucleus of cattle, with a focus on microbial richness, composition, functional diversity, and correlations with blood parameters. Fecal and blood samples from 21 cattle were analyzed using 16S rRNA gene sequencing. Older cattle exhibited greater bacterial diversity and abundance, with significant changes in alpha diversity indices (p < 0.05). Beta diversity analysis revealed significant variations in microbial composition between age groups and the interaction of age and sex (p < 0.05). Correlations between alpha diversity, community composition, and hematological values highlighted the influence of microbiota on bovine health. Beneficial butyrate-producing bacteria, such as Ruminococcaceae, were more abundant in older cattle, suggesting a role in gut health. Functional diversity analysis indicated that younger cattle had significantly more abundant metabolic pathways in fermentation and anaerobic chemoheterotrophy. These findings suggest management strategies including tailored probiotic therapies, dietary adjustments, and targeted health monitoring to enhance livestock health and performance. Further research should include comprehensive metabolic analyses to better correlate microbiota changes with age-related variations, enhancing understanding of the complex interactions between microbiota, age, and reproductive status.
Parasitism-induced changes in microbial eukaryotes of Peruvian alpaca gastrointestinal tract
(MDPI, 2024-01-27) Sanchez, Diana; Zapata Coacalla, Celso; Romero Avila, Yolanda; Flores Huarco, Nils Herber; Oros, Oscar; Alvarado Chuqui, Wigoberto; Quilcate Pairazamán, Carlos Enrique; Guevara Alvarado, Hada María; Estrada Cañari, Richard; Coila Añasco, Pedro Ubaldo
Alpacas, important genetic resources in the Andean region of Peru, are vulnerable to diarrhea caused by pathogenic parasites such as Eimeria lamae and Giardia sp., which can be fatal, especially in neonates, due to their physiological immaturity and limited adaptability. The study investigated the diversity and abundance of intestinal fungi and protists in alpacas infected with Eimeria lamae and Giardia sp. compared to healthy alpacas. A total of 19 alpacas, aged between one and two months, were included. They were divided into two groups, one with pathological conditions (nine) and the other healthy (ten). Parasitological analyses for the detection of parasites and subsequent molecular analysis were performed on the collected fecal samples. The results revealed a greater diversity and abundance of protists in infected alpacas in comparison with healthy alpacas, while the fungal composition did not show significant changes. Therefore, parasitic infections affect the protist component of the alpaca gut microbiota. Also, it was observed that Blastocystis was identified in all healthy alpacas, serving as a possible marker of the health of the intestinal microbiota; in addition, Prussia and Pichia are beneficial fungi that help control diseases. This groundbreaking study in neonatal alpacas is the first to explore potential changes in the intestinal microbiota during an infectious state, underscoring the importance of further research to comprehend its effects on alpaca health and immune responses.
Sex-induced changes in microbial eukaryotes and prokaryotes in gastrointestinal tract of simmental cattle
(MDPI, 2024-11-15) Rojas Cruz, Diorman; Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Figueroa Venegas, Deyanira Antonella; Quilcate Pairazamán, Carlos Enrique; Ganoza Roncal, Jorge Juan; Maicelo Quintana, Jorge Luis; Coila Añasco, Pedro Ubaldo; Alvarado Chuqui, Wigoberto; Cayo Colca, Ilse Silvia
This study investigates gender-based differences in the gut microbiota of Simmental cattle, focusing on bacterial, archaeal, and fungal communities. Fecal samples were collected and analyzed using high-throughput sequencing, with taxonomic classification performed through the SILVA and UNITE databases. Alpha and beta diversity metrics were assessed, revealing significant differences in the diversity and composition of archaeal communities between males and females. Notably, females exhibited higher alpha diversity in archaea, while beta diversity analyses indicated distinct clustering of bacterial and archaeal communities by gender. The study also identified correlations between specific microbial taxa and hematological parameters, with Treponema and Methanosphaera showing gender-specific associations that may influence cattle health and productivity. These findings highlight the importance of considering gender in microbiota-related research and suggest that gender-specific management strategies could optimize livestock performance. Future research should explore the role of sex hormones in shaping these microbial differences.