Contreras Huamani, MijaílOlaguivel Flores, C. A.Guillén Palomino, Crissthel YverlinCarretero, M. I.Fumuso, F. G.Laines Arcce, I. M.2024-01-242024-01-242023-12-06Huamani, M. C.; Olaguivel, C. A.; Palomino, C. G.; Carretero, M. I.; Fumuso, F. G.; & Arcce, I. L. (2023). Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders. Reproduction in Domestic Animals, 00, 1-7. doi: 10.1111/rda.145141439-0531https://hdl.handle.net/20.500.12955/2416The aim of this study was to evaluate alpaca pregnancy outcomes and birth rates of females inseminated with frozen semen using two commercial extenders. A total of 18 ejaculates from 8 adult alpaca males were obtained with artificial vagina, and macroscopic and microscopic semen characteristics were assessed. Afterwards, samples were divided into two aliquots, diluted with Biladyl® B or AndroMed®, and cooled for 2 h at 5°C. At that moment, sperm motility was evaluated, and samples were frozen through a gradual descent of temperature using a liquid nitrogen tank. To analyse frozen sperm quality, samples were thawed at 38°C for 30 s. Even though a significant decrease in sperm motility and viability was detected when thawed (p < .05), no superiority was found between the two commercial extenders (Biladyl® B vs. AndroMed®). A total of 36 alpaca females were artificially inseminated (AI) between 30 and 34 h post-injection of a GnRH analogue, administered when a growing dominant follicle was detected through transrectal palpation and ultrasonography. Obtained pregnancy rates were similar between Biladyl® B (33.3%, 6/18) and AndroMed® (22.2%, 4/18). No significant differences were detected in birth rates between the two tested extenders, obtaining 4 and 3 births for Biladyl® and AndroMed®, respectively. In conclusion, alpaca pregnancies and alive offspring can be obtained through AI with frozen semen at similar efficiency rates using commercial diluents, Biladyl® B or AndroMed®.application/pdfenginfo:eu-repo/semantics/restrictedAccessAlpacaAndroMed®Biladyl® BBirth ratesPregnancySemen cryopreservationinfo:eu-repo/semantics/articlehttps://purl.org/pe-repo/ocde/ford#4.02.01https://doi.org/10.1111/rda.14514AlpacaAlpacasBirth rateTasa de natalidadPregnancyGestaciónSemen preservationConservación del semen