Tsuzuki, RinCabrera Pintado, Rosa MaríaBiondi Thorndike, Jorge AndrésGutiérrez Reynoso, Dina LidaAmasifuen Guerra, Carlos AlbertoGuerrero Abad, Juan CarlosAragón Caballero, Liliana MaríaHuarhua Zaquinaula, Medali HeidiUreta Sierra, CledyAlberca Cruz, Olenka InesElespuru Suna, Milca GianiraBlas Sevillano, Raúl HumbertoTorres Arias, Ines CarolinaFlores Ticona, JoelCáceres de Baldárrago, FátimaRodoríguez Pérez, EnriqueHozum, TakuoSaito, HirokiKotera, ShunsukeAkagi, YasunoriKodama, MotoichiroKomatsu, KenArie, Tsutome2021-01-122021-01-122020-12-28Tsuzuki, R.; Cabrera Pintado, R.M.; Biondi Thorndike, J.A.; Gutiérrez Reynoso, D.L.; Amasifuen Guerra, C.A.; Guerrero Abad, J.C.; Aragón Caballero, L.M.; Huarhua Zaquinaula, M.H.; Ureta Sierra, C.; Alberca Cruz, O.I.; Elespuru Suna, M.G.; Blas Sevillano, R.H.; Torres Arias, I.C.; Flores Ticona, J.; de Baldárrago, F.C.; Pérez, E.R.; Hozumi, T.; Saito, H.; Kotera, S.; Akagi, Y.; Kodama, M.; Komatsu, K.; Arie, T. Mutations found in the Asc1 gene that confer susceptibility to the AAL-Toxin in ancestral tomatoes from Peru and Mexico. Plants 2021, 10, 47. doi: 10.3390/ plants10010047https://repositorio.inia.gob.pe/handle/20.500.12955/1212Tomato susceptibility/resistance to stem canker disease caused by Alternaria alternata f. sp. lycopersici and its pathogenic factor AAL-toxin is determined by the presence of the Asc1 gene. Several cultivars of commercial tomato (Solanum lycopersicum var. lycopersicum, SLL) are reported to have a mutation in Asc1, resulting in their susceptibility to AAL-toxin. We evaluated 119 ancestral tomato accessions including S. pimpinellifolium (SP), S. lycopersicum var. cerasiforme (SLC) and S. lycopersicum var. lycopersicum “jitomate criollo” (SLJ) for AAL-toxin susceptibility. Three accessions, SP PER018805, SLC PER018894, and SLJ M5-3, were susceptible to AAL-toxin. SLC PER018894 and SLJ M5-3 had a two-nucleotide deletion (nt 854_855del) in Asc1 identical to that found in SLL cv. Aichi-first. Another mutation (nt 931_932insT) that may confer AAL-toxin susceptibility was identified in SP PER018805. In the phylogenetic tree based on the 18 COSII sequences, a clade (S3) is composed of SP, including the AAL-toxin susceptible PER018805, and SLC. AAL-toxin susceptible SLC PER018894 and SLJ M5-3 were in Clade S2 with SLL cultivars. As SLC is thought to be the ancestor of SLL, and SLJ is an intermediate tomato between SLC and SLL, Asc1s with/without the mutation seem to have been inherited throughout the history of tomato domestication and breeding.1. Introduction. 2. Results. 3. Discussion. 4. Materials and Methods. 5. Conclusions. Referencesapplication/pdfenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/Solanum pimpinellifoliumSolanum lycopersicum var. cerasiformeAlternaria alternata tomato pathotypeAAL-toxinPerúinfo:eu-repo/semantics/articleBiotecnología agrícola, Biotecnología alimentariaPlantshttps://doi.org/10.3390/plants10010047