Poclín Rojas, Annie YoselinSegura, Gleni TatianaBernilla Carrillo, DianaAmpuero Trigoso, GustavoQuispe Ccasa, Hurley AbelVasquez Tarrillo, Ronald WillDepaz Hino, BenjaminSaucedo Uriarte, José Américo2025-03-312025-03-312025-01-13Poclín-Rojas, A.Y., Segura, G.T., Carrillo, D.B., Ampuero-Trigoso, G., Quispe-Ccasa, H.A., Vásquez-Tarrillo, R.W., Depaz-Hizo, B. and Saucedo-Uriarte, J.A. (2024). Use Intensity of Progesterone Devices for Fixed-time Artificial Insemination in Gyr Crossbred Cows from the Peruvian Tropics. Indian Journal of Animal Research. 1-10. doi: 10.18805/IJAR.BF-1858.http://hdl.handle.net/20.500.12955/2691Background: The implementation of routine fixed-time artificial insemination programs can generate significant waste and costs, which good management practices can help mitigate. The objective of the study was to assess the effects of second- and third-use progesterone devices on estrus characteristics and pregnancy rates in synchronized crossbred Gyr cows. Methods: Ten cows were synchronized with first-use Bovine Intravaginal Progesterone Devices (DIB), nine with second-use DIB and ten with third-use DIB. All cows were ≥90 days postpartum, had an average milk production of 6.9±1.9 L/day and were aged 4-10 years. Estrus signs were recorded at insemination and pregnancies were confirmed at 60 days by ultrasonography. Result: No significant associations were found between DIB use intensity and estrus detection patch response, mucus color, body condition score (CC), calvings, or breed purity (p>0.05). Body condition was associated with uterine turgor tone (p<0.05) and 100% of cows with CC 3.0 showed turgor tone 3. The highest pregnancy rate was found in second-use DIB (55.6%) and CC 2.8 (47.1%), though neither association was significant (p>0.05). DIBs may be used up to a second-use, as cervical mucosal stimulation and pregnancy rate remain unaffected.application/pdfenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/Bovine intravaginal deviceCervical mucusEstrus synchronizationReuseTropical livestock.info:eu-repo/semantics/articlehttps://purl.org/pe-repo/ocde/ford#4.03.01https://purl.org/pe-repo/ocde/ford#4.05.00https://doi.org/10.18805/ijar.bf-1858Ganado Gyr; Ganado cruzado; Inseminación artificial; Progesterona; Dispositivos intravaginales; Sincronización del estro; Moco cervical; Ganado tropical; Perú