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dc.contributor.authorFerreira, C.F-
dc.contributor.authorGutiérrez Reynoso, Dina Lida-
dc.contributor.authorKreuze, Jan-
dc.contributor.authorIskra Caruana, M.L-
dc.contributor.authorChabannes, M-
dc.contributor.authorBarbosa, A.C.O-
dc.contributor.authorSantos, T.A-
dc.contributor.authorSilva, A.G.S-
dc.contributor.authorAmorim, E.P-
dc.contributor.authorDe Oliveira, S.A.S-
dc.contributor.authorJesus, O.N-
dc.coverage.spatialBrasiles_PE
dc.date.accessioned2020-05-05T21:05:46Z-
dc.date.available2020-05-05T21:05:46Z-
dc.date.issued2019-08-14-
dc.identifier.citationFerreira, C.F., Gutierrez, D.L., Kreuze, J.F., Iskra-Caruana, M.L., Chabannes, M., Barbosa, A.C.O., Santos, T.A., Silva, A.G.S., Santos, R.M.F., Amorim, E.P., Oliveira, S.A.S. de, Jesus, O.N. Rapid plant DNA and RNA extraction protocol using a bench drill. Genetics and Molecular Research. 18(3): GMR18394.es_PE
dc.identifier.urihttp://repositorio.inia.gob.pe/handle/20.500.12955/1076-
dc.description.abstractPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.es_PE
dc.description.tableofcontentsINTRODUCTION. MATERIAL AND METHODS. RESULTS AND DISCUSSION. ACKNOWLEDGEMENT. CONFLICTS OF INTEREST. REFERENCES.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherFUNPEC-RPes_PE
dc.relation.ispartofGenetics and Molecular Research. 18(3): GMR18394.es_PE
dc.relation.ispartofseriesGMR;18394-
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.sourceInstituto Nacional de Innovación Agrariaes_PE
dc.source.uriRepositorio Institucional - INIAes_PE
dc.subjectInexpensivees_PE
dc.subjectPlant nucleic acids extractiones_PE
dc.subjectRNA pellet viabilityes_PE
dc.titleRapid plant DNA and RNA extraction protocol using a bench drilles_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.subject.ocdeTecnología de modificación genéticaes_PE
dc.identifier.journalGenetics and Molecular Researches_PE
dc.relation.publisherversionhttps://www.geneticsmr.com/articles/rapid-plant-dna-and-rna-extraction-protocol-using-bench-drilles_PE
dc.publisher.countryBrasiles_PE
dc.identifier.doihttps://doi.org/10.4238/gmr18394-
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