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dc.contributor.authorCastro Gómez, Juan Carlos-
dc.contributor.authorMaddox, J. Dylan-
dc.contributor.authorCobos Ruiz, Marianela-
dc.contributor.authorRequena Anicama, David Ruben-
dc.contributor.authorZimic Peralta, Mirko Juan-
dc.contributor.authorBombarely Gomez, Aureliano-
dc.contributor.authorImán Correa, Sixto Alfredo-
dc.contributor.authorCerdeira Gutiérrez, Luis Alexander-
dc.contributor.authorMedina Bardales, Anderson E.-
dc.coverage.spatialAmazoniaes_PE
dc.coverage.spatialPerúes_PE
dc.date.accessioned2018-09-27T22:33:29Z-
dc.date.available2018-09-27T22:33:29Z-
dc.date.issued2015-
dc.identifier.citationCastro, J. C., Maddox, J. D., Cobos, M., Requena, D., Zimic, M., Bombarely, A., ... Medina, A. E. (2015). De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis. BMC genomics, 16(1): 997. Doi: 10.1186/s12864-015-2225-6es_PE
dc.identifier.issn1471-2164-
dc.identifier.urihttp://repositorio.inia.gob.pe/handle/inia/736-
dc.description.abstractMyrciaria dubia is an Amazonian fruit shrub that produces numerous bioactive phytochemicals, but is best known by its high L-ascorbic acid (AsA) content in fruits. Pronounced variation in AsA content has been observed both within and among individuals, but the genetic factors responsible for this variation are largely unknown. The goals of this research, therefore, were to assemble, characterize, and annotate the fruit transcriptome of M. dubia in order to reconstruct metabolic pathways and determine if multiple pathways contribute to AsA biosynthesis.RESULTS: In total 24,551,882 high-quality sequence reads were de novo assembled into 70,048 unigenes (mean length = 1150 bp, N50 = 1775 bp). Assembled sequences were annotated using BLASTX against public databases such as TAIR, GR-protein, FB, MGI, RGD, ZFIN, SGN, WB, TIGR_CMR, and JCVI-CMR with 75.2 % of unigenes having annotations. Of the three core GO annotation categories, biological processes comprised 53.6 % of the total assigned annotations, whereas cellular components and molecular functions comprised 23.3 and 23.1 %, respectively. Based on the KEGG pathway assignment of the functionally annotated transcripts, five metabolic pathways for AsA biosynthesis were identified: animal-like pathway, myo-inositol pathway, L-gulose pathway, D-mannose/L-galactose pathway, and uronic acid pathway. All transcripts coding enzymes involved in the ascorbate-glutathione cycle were also identified. Finally, we used the assembly to identified 6314 genic microsatellites and 23,481 high quality SNPs. CONCLUSIONS: This study describes the first next-generation sequencing effort and transcriptome annotation of a non-model Amazonian plant that is relevant for AsA production and other bioactive phytochemicals. Genes encoding key enzymes were successfully identified and metabolic pathways involved in biosynthesis of AsA, anthocyanins, and other metabolic pathways have been reconstructed. The identification of these genes and pathways is in agreement with the empirically observed capability of M. dubia to synthesize and accumulate AsA and other important molecules, and adds to our current knowledge of the molecular biology and biochemistry of their production in plants. By providing insights into the mechanisms underpinning these metabolic processes, these results can be used to direct efforts to genetically manipulate this organism in order to enhance the production of these bioactive phytochemicals.The accumulation of AsA precursor and discovery of genes associated with their biosynthesis and metabolism in M. dubia is intriguing and worthy of further investigation. The sequences and pathways produced here present the genetic framework required for further studies. Quantitative transcriptomics in concert with studies of the genome, proteome, and metabolome under conditions that stimulate production and accumulation of AsA and their precursors are needed to provide a more comprehensive view of how these pathways for AsA metabolism are regulated and linked in this species.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherBioMed Centrales_PE
dc.relation.ispartofBMC Genomics,16:997, 2015es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceInstituto Nacional de Innovación Agrariaes_PE
dc.source.uriRepositorio Institucional - INIAes_PE
dc.subjectCamu-camues_PE
dc.subjectMetabolic pathway reconstructiones_PE
dc.subjectNext-generation sequencinges_PE
dc.subjectPlant vitamin C metabolismes_PE
dc.titleDe novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesises_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.subject.ocdeBiotecnología Agrícola y de alimentoses_PE
dc.identifier.journalBMC Genomicses_PE
dc.description.peer-reviewPeer reviewedes_PE
dc.relation.publisherversionhttps://doi.org/10.1186/s12864-015-2225-6es_PE
dc.publisher.countryReino Unidoes_PE
dc.identifier.doi10.1186/s12864-015-2225-6-
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